Association of miRNA-145 with the occurrence and prognosis of hydrosalpinx-induced defective endometrial receptivity

Abstract

MiR-145 is reported to facilitate inflammation and is also associated with unsuccessful embryonic implantation. Whether miR-145 mediates inflammatory response underlying hydrosalpinx-induced defective endometrial receptivity (ER) remains unclear, and this study attempted to clarify this point. Endometrium samples were collected from hydrosalpinx patients (case, n = 243) and patients with tubal patency/obstruction (control, n = 187). The peripheral blood samples of cases and controls were collected to determine the genotypes of miR-145 SNPs. The value of miR-145 expression in the diagnosis and prognostic estimation of hydrosalpinx was assessed using ROC curve and regression analysis, respectively. Lipopolysaccharide (LPS) cell model was established with endometrial cells, and cells were transfected with miR-145 mimic, inhibitor, or negative control. MiR-145 and cytokine levels were quantified by quantitative reverse transcription PCR or western blot. MiR-145 expression was significantly higher in hydrosalpinx compared to control group, and high miR-145 expression was significantly associated with moderate/severe tube lesion, high pulsatility index (>1.06), and high resistance index (>0.61) in hydrosalpinx patients. ROC curve analysis indicated that monitoring miR-145 expression may be useful for the diagnosis of hydrosalpinx (AUC = 0.704). A alleles of rs41291957 (G>A) and rs353292 (G>A) were significantly associated with an increased risk of hydrosalpinx compared to G allele (p < 0.05), yet the mutant allele of rs353291 (A>G) and rs4705343 (T>C) significantly reduced susceptibility to hydrosalpinx compared to the wild type allele. Treatments with miR-145 mimic and LPS in endometrial cells significantly increased the levels of TGF-β1, TNF-α, IL-6, and IL-8 compared to negative control, while treatment with miR-145 inhibitor decreased the cytokine levels. In conclusion, abnormally expressed miR-145 may be involved in hydrosalpinx-induced ER defects by regulating inflammatory response.

MicroRNA Variants miR-27a rs895819 and miR-423 rs6505162, but not miR-124-1 rs531564, are Linked to Endometriosis and its Severity

“…In another study, of 667 miRNAs that were studied in women with endometriosis, two miRNAs including miR-483-5p and miR-629 were highly downregulated in patients compared with controls and the authors of this study considered these miRNAs to have an important role in an early defect in the physiological activity of the proliferative endometrium, which eventually would lead to endometriosis ( 22 ). The importance of miRNAs including miR-145 ( 23 , 24 ), miR-200b-3p ( 25 ), miR-92a ( 26 ), mir-185-5p ( 27 ), microRNA-520 ( 28 ), and miRNA-125b ( 29 ) has been indicated in various studies.…”

Association of miRNA-145 Single Nucleotide Polymorphisms in Abdominal Aortic Aneurysms

“…Data from both experimental and clinical studies suggest that miRNA-145 differentiated levels in patients' serum could serve as an essential biomarker for AAA detection and progression (23). Single nucleotide polymorphisms (SNPs) in miRNA genes (miR-SNPs) could affect miRNA function via either altering transcription of the primary miRNA transcript, or processing of the pri-miRNA and pre-miRNA or by influencing miRNA-mRNA interactions (24)(25)(26)(27); thus, miR-SNPs are implicated in the development of various diseases (28,29). Although a possible link between miRNA-145 and AAA has been indicated, there is no published data regarding the association of miRNA-145 genetic polymorphisms with AAA susceptibility.…”

Proximal tubal occlusion first or oocyte retrieval first for patients with hydrosalpinx?

“…Studies have shown that hydrosalpinx may interfere with embryo implantation by affecting endometrial receptivity [5][6][7]. The possible mechanisms include mechanical scour caused by uid re ux [8], toxic effects of hydrops on embryos [5,7,9], and effects of hydrops on endometrial receptivity [10][11][12]. In addition, enlarged uid can also affect ovarian function.…”