Association of miR-197-5p, a Circulating Biomarker for Heart Failure, with Myocardial Fibrosis and Adverse Cardiovascular Events among Patients with Stage C or D Heart Failure

Abstract

Objective: The aim of this study was to identify heart failure (HF)-specific circulating micro-RNAs (miRNA), and examine whether the selected miRNAs correlate with myocardial fibrosis and are reflective of the incidence of adverse cardiovascular events in patients with stage C or D HF. Methods: Circulating miRNAs which were expressed in end-stage HF patients and matched healthy controls were detected by microarray analysis and validated by quantitative real-time polymerase chain reaction. Multivariate Cox regression analysis was performed to determine whether the selected circulating miRNAs could be prognostic factors in HF patients. Results: In a cohort of 7 healthy controls and 9 patients with stage C or D HF, 7 miRNAs were differentially expressed. These miRNAs were further investigated in a second cohort of 80 patients with stage C or D HF and 30 healthy controls. Only miR-197-5P correlated with fibrosis as seen in cardiac magnetic resonance imaging in patients under the age of 50 years with stage C or D HF (r = 0.42, p = 0.008). Multivariate analyses revealed that miR-197-5P was also a risk factor for composite endpoint events in patients under the age of 50 years with stage C or D HF. Conclusion: miR-197-5P is a circulation miRNA that correlates with MF and adverse cardiac events in HF patients under the age of 50 years.

Long Noncoding RNA FAM225A Promotes Esophageal Squamous Cell Carcinoma Development and Progression via Sponging MicroRNA-197-5p and Upregulating NONO

“…MiR-197-5p was demonstrated to act as circulating biomarker for heart failure [23]. It has been reported that in carcinogenesis of fibrosarcoma, miR-197-5p exerts its regulatory role through inhibiting of KIAA0101 [24].…”

Roles of Biomarkers in Myocardial Fibrosis

“…Rubiś et al found that baseline serum levels of miR-21, miR-26, miR-29, and miR-30 were significantly different in DCM patients than in controls, and that MMP-2 levels were strongly associated with all of the microRNAs studied [137]. In a prospective study, elevated baseline plasma miR-197-5P levels were considered to be associated with myocardial fibrosis in patients with end-stage HF, and during the average 937-day follow-up period, 22 patients (27.5%) had major adverse cardiac events, including 19 deaths and three cardiac transplantations [138]. Additionally, multiple studies have found evidence implicating cardiac interstitial fibrosis in the deterioration of cardiac function in myocardial infarction.…”

Implications of SARS-CoV-2 Mutations for Genomic RNA Structure and Host microRNA Targeting

“…MiR-197-5p is upregulated in patients with cardiovascular disease and has been proposed as a biomarker for the prediction of cardiovascular events [52][53][54]. It is well established that patients with cardiovascular disease are overrepresented in symptomatic COVID-19 cohorts and have a higher mortality rate [55].…”