Association of metabolism‐related genes polymorphisms with adenocarcinoma of the oesophagogastric junction: Evidence from 2261 subjects

Abstract

The etiology of adenocarcinoma of the esophagogastric junction (AEG) remains unclear. It is believed that the increasing of AEG may be correlated with the elevated ratio of obesity and overweight. Thus, metabolism‐related genes and variants may play important roles in the occurrence and progress of AEG. The current investigation involved 720 patients with AEG and 1541 healthy controls. We selected transcription factor 7‐like 2 (TCF7L2) rs7903146 and rs290481, INS rs689 and INSR rs1799817 single‐nucleotide polymorphisms (SNPs), and explored the association of these SNPs with lymph node status and risk of AEG. The polymerase chain reaction was harnessed to identify the genotyping of four polymorphisms. We found that TCF7L2 rs290481 (T > C) and INSR rs1799817 (G > A) polymorphisms were associated with the increased susceptibility of AEG (P = .007 and 0.004 for TCF7L2 rs290481 in TC vs TT and TC/CC vs TT models, and P = .040 for INSR rs1799817 in GA/AA vs GG model). We also conducted a subgroup analysis by different cancer stage. We identified that TCF7L2 rs290481, INS rs689, and INSR rs1799817 SNPs increased the susceptibility of AEG in different cancer stage subgroups. In addition, we found that rs290481 SNP in TCF7L2 gene increased the risk of lymph node metastasis in drinking patients with AEG. However, the association of INSR rs1799817 SNP with a decreased risk of lymph node metastasis in smoking patients with AEG was found. Our findings highlight that TCF7L2 rs290481, INS rs689, and INSR rs1799817 polymorphisms may increase the risk of AEG. In addition, TCF7L2 rs290481 and INSR rs1799817 SNPs may influence the lymph node metastasis in patients with AEG.

Association of leptin receptor polymorphisms with susceptibility of non‐small cell lung cancer: Evidence from 2249 subjects

“…The value of Hardy-Weinberg equilibrium (HWE) was obtained by online goodness-offit chi-square test. [36][37][38][39][40][41] An internet haplotype construction method, SHESIS software (http:// analy sis. bio-x.…”

“…All statistical analyses were performed by using SAS 9.4 software (SAS Institute, Inc.). The value of Hardy–Weinberg equilibrium (HWE) was obtained by online goodness‐of‐fit chi‐square test 36–41 . An internet haplotype construction method, SHESIS software (http://analysis.bio-x.cn/myAnalysis.php), was harnessed to get the haplotypes of LEPR gene 38,42–45 .…”

“…The value of Hardy–Weinberg equilibrium (HWE) was obtained by online goodness‐of‐fit chi‐square test. 36 , 37 , 38 , 39 , 40 , 41 An internet haplotype construction method, SHESIS software ( http://analysis.bio‐x.cn/myAnalysis.php ), was harnessed to get the haplotypes of LEPR gene. 38 , 42 , 43 , 44 , 45 A p < 0.05 was used as the criterion of the significance level.…”

Relationship of microRNA locus with type 2 diabetes mellitus: a case–control study

“…The differencces categorical variables (smoking status, alcohol consumption, BMI, genotypes, sex and age) were measured using chi-square test (χ 2 ). Using the genotype number in controls of rs3746444, rs11614913 and rs2910164, Hardy–Weinberg equilibrium (HWE) was evaluated by an internet-based calculator ( 37 , 38 , 39 , 40 , 41 ). The correlations of rs3746444, rs11614913 and rs2910164 polymorphisms with occurrence of T2DM were determined by odds ratios (ORs) and CIs.…”

Methylenetetrahydrofolate reductase C677T (Ala&gt;Val, rs1801133 C&gt;T) polymorphism decreases the susceptibility of hepatocellular carcinoma: a meta-analysis involving 12,628 subjects

“…By omitting a study one by one and analyzing the remainders, sensitivity analysis was performed to assess the stability of our findings. The distribution of the MTHFR rs1801133 genotype was used to calculate the P value of HWE by using an online software (http://ihg.gsf.de/cgi-bin/hw/hwa1.pl) in controls [33][34][35]. STATA 12.0 software (Stata Corp., College Station, Texas) was used to conduct the analysis.…”