Association of Mesenchymal Stem Cells Derived from Bone Marrow and Adipose Tissue Enhances Bone Repair in Rat Calvarial Defects

Totoli, Gabriela Guaraldo Campos; Bighetti‐Trevisan, Rayana Longo; Freitas, Gileade Pereira; Adolpho, Leticia Faustino; Almeida, Adriana Luisa Golçalves; Barbosa, Ana Carolina Loyola; Ramos, Jaqueline Isadora Reis; Beloti, Márcio Mateus; Rosa, Adalberto Luiz

doi:10.2217/rme-2022-0219

Cited by 6 publications

(2 citation statements)

References 29 publications

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“…It was shown that these treatments resulted in increased bone formation compared to the controls. Both cells had the ability to induce bone tissue formation at comparable amounts, and their association enhances bone repair properties [73].…”

Section: Bone Repairmentioning

confidence: 99%

Adipose-Derived Mesenchymal Stromal Cells: A Tool for Bone and Cartilage Repair

et al. 2023

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The osteogenic and chondrogenic differentiation ability of adipose-derived mesenchymal stromal cells (ASCs) and their potential therapeutic applications in bone and cartilage defects are reported in this review. This becomes particularly important when these disorders can only be poorly treated by conventional therapeutic approaches, and tissue engineering may represent a valuable alternative. Being of mesodermal origin, ASCs can be easily induced to differentiate into chondrocyte-like and osteocyte-like elements and used to repair damaged tissues. Moreover, they can be easily harvested and used for autologous implantation. A plethora of ASC-based strategies are being developed worldwide: they include the transplantation of freshly harvested cells, in vitro expanded cells or predifferentiated cells. Moreover, improving their positive effects, ASCs can be implanted in combination with several types of scaffolds that ensure the correct cell positioning; support cell viability, proliferation and migration; and may contribute to their osteogenic or chondrogenic differentiation. Examples of these strategies are described here, showing the enormous therapeutic potential of ASCs in this field. For safety and regulatory issues, most investigations are still at the experimental stage and carried out in vitro and in animal models. Clinical applications have, however, been reported with promising results and no serious adverse effects.

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Section: Bone Repairmentioning

confidence: 99%

Adipose-Derived Mesenchymal Stromal Cells: A Tool for Bone and Cartilage Repair

et al. 2023

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“…The available treatments for these situations, such as autografts, allografts, or synthetic bone grafts have limited availability, elicit adverse effects, or present low efficiency and, therefore, new strategies to treat bone defects have been investigated by the scientific community. Considering this context, tissue engineering and cell therapy strategies use biomaterials and cells to induce bone repair and among the latter mesenchymal stem cells (MSCs) have been the most studied (Adolpho et al, 2022; Almeida et al, 2019; Beloti et al, 2012; Ferraz et al, 2017; Freitas et al, 2017; Freitas, Lopes, Souza, Oliveira, Almeida, Coelho et al, 2019; Freitas, Lopes, Souza, Oliveira, Almeida, Souza, et al, 2019, 2021; Santos et al, 2015; Souza, Freitas, et al, 2018; Campos Totoli et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Effect of the secretome of mesenchymal stem cells overexpressing BMP‐9 on osteoblast differentiation and bone repair

Calixto,

Freitas,

Souza

et al. 2023

Journal Cellular Physiology

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The secretome present in the conditioned medium (CM) of mesenchymal stem cells (MSCs) is a promising tool to be used in therapies to promote bone regeneration. Considering the high osteogenic potential of the bone morphogenetic protein 9 (BMP‐9), we hypothesized that the secretome of MSCs overexpressing BMP‐9 (MSCsBMP‐9) enhances the osteoblast differentiation of MSCs and the bone formation in calvarial defects. CM of either MSCsBMP‐9 (CM‐MSCsBMP‐9) or MSCs without BMP‐9 overexpression (CM‐MSCsVPR) were obtained at different periods. As the CM‐MSCsBMP‐9 generated after 1 h presented the highest BMP‐9 concentration, CM‐MSCsBMP‐9 and CM‐MSCsVPR were collected at this time point and used to culture MSCs and to be injected into mouse calvarial defects. The CM‐MSCsBMP‐9 enhanced the osteoblast differentiation of MSC by upregulating RUNX2, alkaline phosphatase (ALP) and osteopontin protein expression, and ALP activity, compared with CM‐MSCsVPR. The CM‐MSCsBMP‐9 also enhanced the bone repair of mouse calvarial defects, increasing bone volume, bone volume/total volume, bone surface, and trabecular number compared with untreated defects and defects treated with CM‐MSCsVPR or even with MSCsBMP‐9 themselves. In conclusion, the potential of the MSCBMP‐9‐secretome to induce osteoblast differentiation and bone formation shed lights on novel cell‐free‐based therapies to promote bone regeneration of challenging defects.

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Protective effects of emodin on subchondral bone and articular cartilage in osteoporotic osteoarthritis rats: A preclinical study

Wei,

Kang,

et al. 2024

Experimental Gerontology

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Association of Mesenchymal Stem Cells Derived from Bone Marrow and Adipose Tissue Enhances Bone Repair in Rat Calvarial Defects

Cited by 6 publications

References 29 publications

Adipose-Derived Mesenchymal Stromal Cells: A Tool for Bone and Cartilage Repair

Adipose-Derived Mesenchymal Stromal Cells: A Tool for Bone and Cartilage Repair

Effect of the secretome of mesenchymal stem cells overexpressing BMP‐9 on osteoblast differentiation and bone repair

Protective effects of emodin on subchondral bone and articular cartilage in osteoporotic osteoarthritis rats: A preclinical study

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