Association of mast cells and bone marrow reticulin fibrosis in patients with bcr-abl negative chronic myeloproliferative neoplasms

Keski, Hakan

doi:10.1016/j.bcmd.2020.102420

Cited by 4 publications

(2 citation statements)

References 28 publications

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“…The proliferation and activation of mast cells are associated with fibrosis in various clinical diseases [ 72 – 74 ]. An increased number of mast cells is observed in MPN samples [ 75 ]. Consistently, the increased presence of mast cells in pre-PMF observed in our study may imply a potentially more challenging prognosis for pre-PMF than for ET.…”

Section: Discussionmentioning

confidence: 99%

Multi-omics differences in the bone marrow between essential thrombocythemia and prefibrotic primary myelofibrosis

Zhang,

Sun,

et al. 2024

Clin Exp Med

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Essential thrombocythemia (ET) and prefibrotic primary myelofibrosis (pre-PMF) are Philadelphia chromosome-negative myeloproliferative neoplasms. These conditions share overlapping clinical presentations; however, their prognoses differ significantly. Current morphological diagnostic methods lack reliability in subtype differentiation, underlining the need for improved diagnostics. The aim of this study was to investigate the multi-omics alterations in bone marrow biopsies of patients with ET and pre-PMF to improve our understanding of the nuanced diagnostic characteristics of both diseases. We performed proteomic analysis with 4D direct data-independent acquisition and microbiome analysis with 2bRAD-M sequencing technology to identify differential protein and microbe levels between untreated patients with ET and pre-PMF. Laboratory and multi-omics differences were observed between ET and pre-PMF, encompassing diverse pathways, such as lipid metabolism and immune response. The pre-PMF group showed an increased neutrophil-to-lymphocyte ratio and decreased high-density lipoprotein and cholesterol levels. Protein analysis revealed significantly higher CXCR2, CXCR4, and MX1 levels in pre-PMF, while APOC3, APOA4, FABP4, C5, and CFB levels were elevated in ET, with diagnostic accuracy indicated by AUC values ranging from 0.786 to 0.881. Microbiome assessment identified increased levels of Mycobacterium, Xanthobacter, and L1I39 in pre-PMF, whereas Sphingomonas, Brevibacillus, and Pseudomonas_E were significantly decreased, with AUCs for these genera ranging from 0.833 to 0.929. Our study provides preliminary insights into the proteomic and microbiome variations in the bone marrow of patients with ET and pre-PMF, identifying specific proteins and bacterial genera that warrant further investigation as potential diagnostic indicators. These observations contribute to our evolving understanding of the multi-omics variations and possible mechanisms underlying ET and pre-PMF.

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Section: Discussionmentioning

confidence: 99%

Multi-omics differences in the bone marrow between essential thrombocythemia and prefibrotic primary myelofibrosis

Zhang,

Sun,

et al. 2024

Clin Exp Med

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“…Instead, they circulate as immature progenitors via the circulatory system to complete their development peripherally in connective or mucosal tissues. 7,8 Many inflammatory cells surround tumour cells, including mast cells, which can secrete many such proangiogenic factors, leading to endothelial cell proliferation and angiogenesis. 8 Studies expressing the significance of MVD in specific MPNs like CML and PMF are done in the past; however, studies to explore the relationship between mast cells, blasts, bone marrow fibrosis and MVD are seldom available in literature.…”

Section: Introductionmentioning

confidence: 99%

Study of significance of bone marrow microvessel density in myeloproliferative neoplasms in correlation with CD34 blasts, mast cell count and fibrosis

et al. 2023

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Background: Myeloproliferative neoplasms (MPN) are clonal hematopoietic stem cell diseases characterised by myeloid cell growth from one or more lineages. Angiogenesis, in contrast to other subtypes, plays a substantial role in the pathophysiology of primary myelofibrosis (PMF). Research expressing the correlation of microvessel density (MVD), blasts, fibrosis and mast cell count in MPN cases are rarely conducted. We aimed to study the significance of MVD in correlation with CD34 blasts, mast cells and fibrosis in bone marrow biopsies of MPN patients. Methods: The current research was a cross sectional study conducted on 66 cases diagnosed as MPN during a six-year period. This comprised of 32 chronic myeloid leukemia (CML), 31 PMF and three essential thrombocythemia (ET) cases. Routine staining along with reticulin stain to look for fibrosis and immunohistochemistry (IHC) using CD34 and mast cell tryptase (MCT) were performed. Results: We found increased MVD in PMF, when compared to CML and ET (p = 0.042). Further, mean MVD was observed to be increased with high blast counts (p = 0.036). On follow up, raised mean MVD was seen in those cases with relapse/deceased as compared to disease-free patients, which was highly significant (p = 0.000). Conclusions: Increased MVD score was mostly associated with PMF subtype among all the MPNs. Further, higher MVD was observed to be associated with increased blast count and poor prognosis. With angiogenesis playing a critical role in disease outcome, we now have drugs to regulate angiogenesis that are supported by contemporary research. However, further studies with larger cohorts to establish the theranostic role of MVD in MPNs is recommended.

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Mast cells as a therapeutic target in myeloproliferative neoplasms

Toledo¹,

Chatain²,

Zenke³

et al. 2022

Trends in Molecular Medicine

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Association of mast cells and bone marrow reticulin fibrosis in patients with bcr-abl negative chronic myeloproliferative neoplasms

Cited by 4 publications

References 28 publications

Multi-omics differences in the bone marrow between essential thrombocythemia and prefibrotic primary myelofibrosis

Multi-omics differences in the bone marrow between essential thrombocythemia and prefibrotic primary myelofibrosis

Study of significance of bone marrow microvessel density in myeloproliferative neoplasms in correlation with CD34 blasts, mast cell count and fibrosis

Mast cells as a therapeutic target in myeloproliferative neoplasms

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