Association of long non-coding RNA H19 and microRNA-21 expression with the biological features and prognosis of non-small cell lung cancer

Abstract

In recent years, long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) have been shown to play important roles in tumor biological function. The aim of this study was to investigate the diagnostic and prognostic value of lncRNA H19 and miR-21 expression in non-small-cell lung cancer (NSCLC). H19 and miR-21 expression was measured in tumor tissues and corresponding non-tumor lung tissues from 200 patients by quantitative reverse transcription polymerase chain reaction. Moreover, the in vitro and in vivo effects of H19 knock out in A549 cells were investigated. Expression of both H19 and miR-21 was significantly higher in lung tissues from patients with NSCLC than in normal lung tissues. Increased expression of H19 and miR-21 was positively correlated with advanced tumor-node-metastasis stage and tumor size. miR-21 expression was highest in stage I and II NSCLC, whereas H19 expression was highest in stage III and IV NSCLC. Knockout of H19 significantly inhibited NSCLC cell proliferation both in vitro and in vivo. The results show that H19 may mainly contributes to the progression of NSCLC, and its expression levels can reflect the invasive and metastatic status to some extent. miR-21 expression more likely plays an important role in early stage NSCLC. Moreover, H19 and miR-21 interact in the regulation of NSCLC, and with greater expression of both H19 and miR-21, overall survival decreased. The combination of H19 and miR-21 may have diagnostic value in NSCLC and represent a target for new NSCLC treatments.

Upregulated lncRNA ADAMTS9‐AS2 suppresses progression of lung cancer through inhibition of miR‐223‐3p and promotion of TGFBR3

“…Currently, the main treatments for lung cancer include surgical excision, chemotherapy and chest radiotherapy. However, the efficacies of these therapies are limited, and novel approaches are needed to improve patients’ life quality and survival . Recent studies suggest that several lncRNAs are dysregulated in multiple cancers, including lung cancers .…”

Clinical Impact of Oncomirs 221 and 222 on Breast Cancer Diagnosis

“…In the current study, miRNA-221 and miRNA-222 are homologous miRNAs located in tandem, within 1 kb from each other, on human X chromosome [14,15]. Recent studies stated that microRNA-221 and miRNA-222 is aberrantly expressed in various malignancies [16] and was found to be overexpressed in triplenegative breast cancer patients [17]. MiRNA-221 and miRNA-222 are thought to serve as oncomiRs because they inhibit many tumor suppressors [18,19,20,21].…”

Polymorphism in lncRNA AC008392.1 and its interaction with smoking on the risk of lung cancer in a Chinese population

“…Many studies have shown that lncRNAs were deregulated in lung cancer, such as MALAT1 (NEAT2), 44 , 45 HOTAIR, 46 SOX2-OT, 47 and H19. 48 MALAT1 could be associated with prognostic parameter for poor survival 49 and cell migration 50 in lung cancer cases. Compared with normal lung tissue, the expression of HOTAIR was higher in lung cancer tissues.…”