Association of lncRNA SH3PXD2A-AS1 with preeclampsia and its function in invasion and migration of placental trophoblast cells

Chen, Qian; Jiang, Sijia; Liu, Haihua; Gao, Yue; Yang, Xiaoxue; Ren, Zhonglu; Gao, Yunfei; Xiao, Lü; Hu, Haoyue; Yu, Yang; Yang, Xinping; Zhong, Ming

doi:10.1038/s41419-020-02796-0

Cited by 28 publications

(20 citation statements)

References 73 publications

(71 reference statements)

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“…Increasing evidence has revealed the key roles of lncRNAs in proliferation, survival, angiogenesis, migration, and cancer cell invasion [ 69 ]. In the last few years, functional studies on well-characterized choriocarcinoma cell lines representing villous and extravillous trophoblast phenotype (BeWo, JAR, and JEG-3), first-trimester trophoblast cell lines (HTR-8/SVneo and Swan-71), and primary trophoblast cell cultures reported some lncRNAs involved in regulatory mechanisms in trophoblasts activity, such as H19, HOTAIR, MALAT1, SPRY4-IT1, and MEG3 [ 70 ]. The potential mechanisms underlying the action of these and others lncRNAs in trophoblasts will be discussed in each pathology to elucidate the potential role of these molecules.…”

Section: Etiopathogenesis Of Placental Related Diseasesmentioning

confidence: 99%

“…Numerous reports have described the aberrant expression of lncRNAs in plasma or placentae samples collected from women with PE ( Figure 1 ). For instance, the lncRNA SH3PXD2A-AS1, which was found upregulated in term placentae from PE women, is reported to be involved in placenta development by recruiting the CCCTC-binding factor (CTCF) to the promoter regions of SH3PXD2A and CCR7, thus inhibiting the transcription of these two essential factors that are involved in invasion and migration of early trophoblast cells [ 70 ]. Another lncRNA described as upregulated in placentas from PE patients is the lncRNA H19 [ 72 ].…”

Section: Lncrnas In Preeclampsiamentioning

confidence: 99%

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The Role of Long Non-Coding RNAs in Trophoblast Regulation in Preeclampsia and Intrauterine Growth Restriction

Monteiro

Peñailillo

Sánchez

et al. 2021

Genes

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Preeclampsia (PE) and Intrauterine Growth Restriction (IUGR) are two pregnancy-specific placental disorders with high maternal, fetal, and neonatal morbidity and mortality rates worldwide. The identification biomarkers involved in the dysregulation of PE and IUGR are fundamental for developing new strategies for early detection and management of these pregnancy pathologies. Several studies have demonstrated the importance of long non-coding RNAs (lncRNAs) as essential regulators of many biological processes in cells and tissues, and the placenta is not an exception. In this review, we summarize the importance of lncRNAs in the regulation of trophoblasts during the development of PE and IUGR, and other placental disorders.

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Section: Etiopathogenesis Of Placental Related Diseasesmentioning

confidence: 99%

Section: Lncrnas In Preeclampsiamentioning

confidence: 99%

The Role of Long Non-Coding RNAs in Trophoblast Regulation in Preeclampsia and Intrauterine Growth Restriction

Monteiro

Peñailillo

Sánchez

et al. 2021

Genes

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“…Among those, hsa_circ_0019868 [SH3 and PX domain-containing protein 2A (SH3PXD2A) gene] may be associated with MPP pathogenesis. SH3PXD2A is an adapter protein involved in the invasiveness of cancer cells that mediates the neurotoxic effect of the amyloid-β peptide (37). The higher expression in MPP groups may enhance the invasiveness of M. pneumoniae.…”

Section: Relative Gene Expression Levelsmentioning

confidence: 99%

Ribosomal RNA‑depleted RNA sequencing reveals the pathogenesis of refractory Mycoplasma pneumoniae pneumonia in children

et al. 2021

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Pneumonia caused by Mycoplasma pneumoniae (M. pneumoniae) is a major cause of community-acquired pneumonia in children. In some cases, M. pneumoniae pneumonia (MPP) can develop into refractory MPP (RMPP), which shows no clinical or radiological response to macrolides, and can progress to severe and complicated pneumonia. However, the pathogenesis of RMPP remains poorly understood. The present study aimed to identify target genes that could be used as biomarkers for the clinical diagnosis of early-stage RMPP through high-throughput sequencing technology. The differences in long non-coding (lnc)RNAs, mRNAs and circular (circ)RNAs were examined between whole-blood samples from two patients with non-refractory MPP (NRMPP), two patients with RMPP and three healthy children using ribosomal (r)RNA-depleted RNA-sequencing techniques and an integrated mRNA/circRNA analysis. A total of 17 lncRNAs (four upregulated and 13 downregulated), 18 mRNAs (six upregulated and 12 downregulated) and 24 circRNAs (12 upregulated and 12 downregulated) were the most significantly differentially expressed (P<0.05) between the NRMPP and RMPP groups. Upon functional analysis, the significantly differentially expressed genes encoded by the targeting mRNAs (prostaglandin-endoperoxide synthase 2, IL-8 and fos-like antigen 1) were screened and identified to be enriched in the 'IL-17 signaling pathway'. Furthermore, the key circRNAs in the NRMPP and RMPP comparative groups were primarily enriched in 'herpes simplex virus 1 infection', 'viral carcinogenesis' and 'RNA transport'. In the present study, a comprehensive analysis of the differences between the NRMPP and RMPP cases was performed based on rRNA-depleted RNA-sequencing techniques, and the selected genes and circRNAs may be closely associated with the complex pathogenesis of RMPP.

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“…Clinical and pathological studies previously confirmed that placental dysplasia, excessive maternal inflammation and endothelial dysfunction are central to the pathogenesis of PE ( 2 , 5 ). Accumulating evidence has also shown that the etiology of PE involves poor placentation due to inadequate trophoblast invasion ( 6 , 7 ). Therefore, it is imperative to perform preclinical experiments in trophoblasts to elucidate the mechanism underlying PE pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

Role of lncRNA BCYRN1 in trophoblast cell physiology and pathogenesis of preeclampsia

et al. 2021

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Long non-coding RNAs (lncRNAs) may play a key role in the pathogenesis of preeclampsia (PE). The present study investigated the role of the lncRNA brain cytoplasmic RNA 1 (BCYRN1) in PE. A total of 30 patients with severe PE (SPE) and 30 patients with mild PE (MPE) were recruited, whilst 30 healthy pregnant individuals were enrolled as controls. Placental tissues of enrolled subjects were collected after delivery. The clinical data of pregnant women and newborns were recorded before the correlation between BCYRN1 expression and clinical characteristics was analyzed. Furthermore, HTR-8/SVneo cells were transfected with BCYRN1 overexpression plasmids and BCYRN1 small interfering (si)RNA. Cell Counting Kit-8, Transwell, flow cytometry and tube formation assays were used to detect the function of BCYRN1 in HTR-8/SVneo cells. Reverse transcription-quantitative PCR was used to detect BCYRN1 expression in placental tissues and HTR-8/SVneo cells. Western blotting was used to detect the protein expression levels of Wnt1 and β-catenin. BCYRN1 expression was lower in placenta with mild PE compared with in normal placenta, and was in turn lower in placenta with severe PE. BCYRN1 was negatively correlated with systolic blood pressure and 24-h urinary protein in patients with PE. BCYRN1 siRNA inhibited cell viability, migration, invasion and tube forming abilities whilst increasing apoptosis. By contrast, BCYRN1 overexpression conferred opposite effects. The levels of Wnt1 and β-catenin expression in the cells and placental tissues were next measured. Cells overexpressing BCYRN1 were further treated with the Wnt pathway inhibitor XAV939. Wnt1 and β-catenin expression were elevated when BCYRN1 was overexpressed, but were decreased after BCYRN1 knockdown. XAV939 attenuated the effect of BCYRN1 overexpression on HTR-8/SVneo cells. Overall, the resulted indicated that upregulation of BCYRN1 increased trophoblast viability and prevented apoptosis by activating the Wnt/β-catenin pathway to delay PE onset.

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Association of lncRNA SH3PXD2A-AS1 with preeclampsia and its function in invasion and migration of placental trophoblast cells

Cited by 28 publications

References 73 publications

The Role of Long Non-Coding RNAs in Trophoblast Regulation in Preeclampsia and Intrauterine Growth Restriction

The Role of Long Non-Coding RNAs in Trophoblast Regulation in Preeclampsia and Intrauterine Growth Restriction

Ribosomal RNA‑depleted RNA sequencing reveals the pathogenesis of refractory Mycoplasma pneumoniae pneumonia in children

Role of lncRNA BCYRN1 in trophoblast cell physiology and pathogenesis of preeclampsia

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