Association of lipase lipoprotein polymorphisms with high-density lipoprotein and triglycerides in elderly men

Abstract

ABSTRACT. Lipoprotein lipase is essential for triglyceride hydrolysis. The polymorphisms S447X in exon 9 and HindIII in intron 8 have been associated with lower triglyceride levels and lower cardiovascular risk in adult men. We examined the association of these lipoprotein lipase polymorphisms with high-density lipoprotein (HDL) and triglyceride levels in elderly men. Blood samples were obtained from 87 elderly men, 48 of whom had cardiovascular disease and 39 (controls) had no history of cardiovascular events. The lipoprotein lipase polymorphisms were analyzed by PCR-RFLP. Allele frequencies were H-= 27.9% and X = 21.5%. There were no significant differences in allele frequencies or blood lipid levels between cardiovascular disease and control groups. However, the X allele was associated with a lower triglyceride/HDL ratio, 2.30 vs 3.02 for X allele absent (P = 0.03); the H-X haplotype was associated with lower triglyceride levels compared to the H+S haplotype (1.22 vs 1.58 mM, respectively) and a lower triglyceride/HDL ratio (2.29 vs 3.26, respectively). The X allele and H-X haplotype were associated with lower triglyceride/HDL ratios in these elderly men, independent of the history of cardiovascular events.

Association of LPL gene variant and LDL, HDL, VLDL cholesterol and triglyceride levels with ischemic stroke and its subtypes

“…Amer et al [28] have also reported a significant increase in plasma levels of TG and significant decrease in HDL in CAD patients bearing HindIII (++) genotype. However, on contrary Araujo et al [29] reported an association of HindIII polymorphism with lower TG levels and cardiovascular risk in elderly men. HDL concentrations and the extent of coronary heart diseases have been reported to be inversely related because HDL particles play a crucial role in reverse cholesterol transport [14,15,30].…”

Association of Lipid Profile, Atherogenic Indices, and LPL Hind-III Gene Polymorphism with Coronary Artery Disease Positive Subjects

“…Similar results, like LPL H + H + genotype, was a risk factor for myocardial infarction in Brazil and Russian population 65 and significantly associated with myocardial infarction (MI) patients in South Indian population 66 , but these polymorphic studies are very limited in India. In another study observed H¯ (G) allele was not associated with blood lipids or cardiovascular events 67 and also a recent study conducted by Marcia et al reported H + /H + (T/T) genotype and the H + (T) allele were associated with elevated VLDLc and triglycerides levels (P < 0.05) and reduced HDL-C levels (P < 0.05) 68 . Likewise, H¯ H¯ (G/G), H + H¯ (T/G) and H + H + (T/T) genotypes of intron 8 T >G LPL gene no significant differences in the serum levels of TC, TG, HDL-c and LDL-c in Saudi Population 69 similarly a study between the control and coronary artery disease in Shiraz City observed, this polymorphism doesn't have any significant association with CAD 70 .…”

Hindiii and S447x Polymorphisms of Lipoprotein Lipase Gene and Their Relationship to Coronary Artery Disease

“…As regards the HindIII polymorphism, the presence of the mutant H-allele did not show any influence on the levels of the different parameters of the lipid profile. Such findings proved that the location of HindIII polymorphism in the middle of intron 8 makes it a non functional polymorphism with almost negligible direct effect on LPL activity (Antonio et al, 2013;Araújo et al, 2010;Chen et al, 2008). gene variants which they consider the main contributors to the change in LPL activity.…”