Association of KCNJ11 rs5219 gene polymorphism with type 2 diabetes mellitus in a population of Syria: a case-control study

Abstract

Background Type 2 diabetes mellitus is believed to be a polygenic disorder that develops as a result of a complex interaction between multiple genes and environmental factors. KCNJ11 gene encodes a Kir6.2 protein which forms the inner section of the potassium channels in pancreatic beta cells. Several studies found that KCNJ11 polymorphism increases T2DM risk. Our study aimed to investigate the association between rs5219 polymorphism of the KCNJ11 gene and T2DM in Syrian patients. Methods This case-control study involved 75 T2DM patients and 63 healthy controls. The KCNJ11 rs5219 polymorphism was genotyped by Restriction Fragment Length Polymorphism (RFLP). Results The frequency of the risk allele K was similar between the two groups (38.7% vs. 38.1%, P = 0.132). The frequency of the KK genotype was higher among the patients’ group (16% vs. 4.8%), and the frequency of the EK genotype was higher among the control group (45.3% vs. 66.6%); however, the differences were statistically insignificant. The KK genotype was significantly associated with T2DM in the recessive model with an OR of 3.81 (95% CI 1.024–14.17, P = 0.035). Conclusions This study showed that rs5219 polymorphism of the KCNJ11 gene is an important risk factor for type 2 diabetes mellitus in a sample of the Syrian population.

Association of KCNJ11 and ABCC8 single-nucleotide polymorphisms with type 2 diabetes mellitus in a Kinh Vietnamese population

“…The genotyping of rs5219 was performed as previously described. [ 20 ] Rs2285676 was genotyped using restriction fragment length polymorphism polymerase chain reaction (PCR) with BcnI enzyme (Thermo Scientific, Waltham, MA, United States). The detailed protocol for genotyping rs2285676 is described in Supplementary Table S1, http://links.lww.com/MD/H879 .…”

Identification SNP rs5219 KCNJ11 gene and blood glucose levels in type 2 diabetes mellitus patients at Moewardi Hospital Surakarta

“…The quality of DNA was analyzed using gel electrophoresis (Bio-rad, USA) and ultraviolet-visible spectrophotometry. Amplification of KCNJ11 gene was done by polymerase chain reaction (PCR) method using forward and reverse primers in the following conditions: Initial denaturation at 95 ∘ C for 5 minutes, denaturation at 95 ∘ C for 30 seconds, annealing at 60 ∘ C for 30 seconds, extensions at 72 ∘ C for 30 seconds and final extensions at 72 ∘ C for 9 minutes (35 cycles) (Makhzoom et al, 2019). The product of the PCR was analyzed by using electrophoresis.…”

Identifying the association between single nucleotide polymorphisms in KCNQ1, ARAP1, and KCNJ11 and type 2 diabetes mellitus in a Chinese population

“…There are varying reports of the association between T2DM and the rs5219 and rs5210 genotypes in KCNJ11 in different populations [11,[32][33][34][35][36][37][38][39]. Moreover, this association has yielded different results in the same population as well.…”