Association of Intermediate T Cell Receptor Cells, Mainly Their NK1.1− Subset, with Protection from Malaria

Weerasinghe, Anura; Sekikawa, Hiroho; Watanabe, Hirotaka; Mannoor, Kaiissar; Morshed, Sufi; Halder, Ramesh C.; Kawamura, Toshihiko; Kosaka, Takayuki; Miyaji, Chikako; Kawamura, Hiroki; Seki, Shuji; Abo, Toru

doi:10.1006/cimm.2000.1737

Cited by 32 publications

(34 citation statements)

References 31 publications

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“…[5][6][7][8][9] Our previous [1][2][3] and present data support these findings. In the case of human malaria, CD36 antigens (sequestrin) on erythrocytes are known to mediate adherence and subsequent infection of Plasmodium falciparum.…”

Section: Discussionsupporting

confidence: 89%

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Protection against malaria by anti‐erythropoietin antibody due to suppression of erythropoiesis in the liver and at other sites

et al. 2005

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SummaryWe have previously reported that erythropoiesis commences in the liver and spleen after malarial infection, and that newly generated erythrocytes in the liver are essential for infection of malarial parasites as well as continuation of infection. At this time, erythropoietin (EPO) is elevated in the serum. In the present study, we administered EPO or anti-EPO antibody into C57BL/6 (B6) mice to modulate the serum level of EPO. When mice were infected with a non-lethal strain (17NXL) of Plasmodium yoelii (blood-stage infection of 10 4 parasitized erythrocytes per mouse), parasitemia continued for 1 month, showing a peak at day 17. Daily injection of EPO (200 IU/day per mouse) from day five to day 14 prolonged parasitemia, whereas injection of anti-EPO antibody (1.5 mg/day per mouse) every second day from day five to day 28 decreased it. Erythropoiesis was confirmed in the liver, spleen and bone marrow by the appearance of nucleated erythrocytes (TER119+). When anti-EPO antibody was injected by the same protocol into mice infected with a lethal strain (17XL) of P. yoelii , all mice showed decreased parasitemia and recovered from the infection. These results suggest that the use of anti-EPO antibody after malarial infection may be of therapeutic value in severe cases of malaria.

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Section: Discussionsupporting

confidence: 89%

“…Parasites were maintained by routine in vivo passages in bone marrow cells. 3 Mice were infected by an i.p. injection of 10 4 parasitized erythrocytes per mouse.…”

Section: Mice and Parasitesmentioning

confidence: 99%

Protection against malaria by anti‐erythropoietin antibody due to suppression of erythropoiesis in the liver and at other sites

et al. 2005

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“…The mice were maintained at the animal facility of Niigata University (Niigata, Japan) under specific pathogen-free conditions. P. yoelii 17XNL (non-lethal strain) was used (29). Parasites were maintained by routine passages in mice.…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA was extracted from liver and spleen tissues of B6 mice (29). To detect mRNAs of IFNγ, IL-12P40, IL-6, TNFα, IL-4 and IL-10, RNA was subjected to reverse transcription using primers of these genes and such cDNA was further amplified by the PCR method (29). Briefly, total RNA was prepared from (30 mg) samples from liver and spleen tissues of control and infected mice by using Isogen (Nippon Gene, Tokyo, Japan).…”

Section: Reverse Transcription (Rt)-pcr For Cytokines Mrnamentioning

confidence: 99%

Cytokine profile of murine malaria: stage-related production of inflammatory and anti-inflammatory cytokines

2011

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Balance between inflammatory and anti-inflammatory cytokines may be important in malaria presentation and outcome. To clarify cytokine interactions that produce pathology of malaria and control infection, C57BL/6 mice were infected with 10 4 parasitized RBCs from a non-lethal strain of Plasmodium yoelii. Kinetics was monitored showing the course of parasitemia, and cytokines were determined by RT-PCR from liver and spleen tissues. Inflammatory cytokines such as interferon-γ (IFNγ), interleukin (IL)-12, IL-6, tumor necrosis factor-α (TNFα) and anti-inflammatory cytokines, including IL-4 and IL-10, were investigated as key molecules that interact with immune cells in the activation of the immune responses. The production of IFNγ mRNA was found to be higher on day 7 than on day 21 after infection, and IL-12 and IL-6 showed higher expression in the liver than in the spleen. Though TNFα was highly expressed on day 14 after infection and on day 21 in the liver, such expression was decreased on day 21 in the spleen. Anti-inflammatory cytokines showed high expression in both the liver and spleen. The results suggest that a relative balance between inflammatory and anti-inflammatory cytokines is crucial and that the increase of inflammatory cytokine levels during the acute phase of malaria may reflect an early and effective immune response. The counteraction effect of anti-inflammatory cytokines is thought to play a role in limiting progression from uncomplicated malaria to severe life-threatening complications.

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“…11,12 Moreover, the tolerogenic hepatic microenvironment, which is rich in immune regulatory mediators and tolerance-promoting APCs such as LSECs, may influence even the function of previously activated T cells in the liver. Previous reports demonstrated that T-cell immunity can be induced in the liver, 13,14 but formal evidence of the continuous presence of memory T cells and their activation by local APC populations in vivo is lacking. We addressed this question by injecting mice with aCD3eAb, which allowed us to study in vivo rapid T-cell activation in peripheral organs that was dependent on Fcc receptor (FccR) cross-linking through APCs.…”

Section: M M U N O L O G Y O R I G I N a L A R T I C L Ementioning

confidence: 99%

Rapid and preferential distribution of blood‐borne αCD3εAb to the liver is followed by local stimulation of T cells and natural killer T cells

et al. 2005

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high CD8+ T cells were activated by aCD3eAb and expressed interferon (IFN)-c.Again, IFN-c release from NKT/T cells was at least as efficient in the liver as in the spleen. Taken together, our results support the notion that the combination of extensive hepatic vascularization and very high scavenger activity allows the liver to fulfill its metabolic tasks and to promote stimulation of the large but widely distributed hepatic population of NKT/T cells.

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Association of Intermediate T Cell Receptor Cells, Mainly Their NK1.1− Subset, with Protection from Malaria

Cited by 32 publications

References 31 publications

Protection against malaria by anti‐erythropoietin antibody due to suppression of erythropoiesis in the liver and at other sites

Protection against malaria by anti‐erythropoietin antibody due to suppression of erythropoiesis in the liver and at other sites

Cytokine profile of murine malaria: stage-related production of inflammatory and anti-inflammatory cytokines

Rapid and preferential distribution of blood‐borne αCD3εAb to the liver is followed by local stimulation of T cells and natural killer T cells

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