Association of Interleukin-1β and Gene Polymorphisms with Liver Pathogenesis in Hepatitis B Virus Infection among Eastern Indian Population

Abstract

Background: Interleukin-1b (IL-1b) is an important member of the family of the proinflammatory cytokines that modulate outcome of hepatitis B virus (HBV) infection. Objectives: This study was designed to investigate the relationship between the polymorphic genotypes of the interleukin-1b (IL-1b) promoter region and the interleukin-1 receptor antagonist gene (IL-1RN) and disease outcome in HBV-infected individuals. Methods: DNA was extracted from 395 study subjects including HBV carriers with varying clinical presentations, as well as healthy controls and spontaneously recovered cases (SRC). Polymorphisms in IL-1b (at position À511) and IL-1RN (variable nucleotide tandem repeats, VNTR) were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and PCR based assay respectively. Results: Among the study subjects, different IL-1b (at position À511) (CC, CT and TT) and IL-1RN (1/1, 1/2, 2/2 and 1/3) polymorphic genotypes were found at variable proportions. Logistic regression analysis revealed, no notable difference at the level of IL-1b promoter (P = 0.244; OR = 0.78; 95% CI = 0.52-1.18) or IL-1RN genotype polymorphism (P = 0.840; OR = 1.03; 95% CI = 0.78-1.36) among the HBV carriers and controls or SRC cases. Pairwise proportion testing showed, IL-1b À511 genotype CC was significantly higher among asymptomatic carriers (ASC) in comparison with liver cirrhosis (LC) patients (P value = 0.028) and healthy control group (P-value = 0.036). IL-1RN genotype 2/2 was considerably higher in LC group than SRC as well as control group. Combinations of IL-1b (À511) and IL-1RN polymorphisms were associated with disease progression, such as CC-1/2 with ASC and TT-2/2 with LC. Conclusion: IL-1b polymorphisms are found to be associated with disease severity. Different polymorphic combinations are associated with degree of disease severity. Overall this is the first report from Eastern India, which shows association of IL-1b polymorphisms with HBV-related hepatic complications. ( J CLIN EXP HEPATOL 2013;3:281-287)

Interleukin‐1 gene cluster Haplotype analysis in the chronic outcome prediction of the Hepatitis B virus infection

“…A protective role was reported for the C/C genotype of the promoter region −511 and therefore less probability of the chronic hepatitis outcome in the Chinese population . In the Indian population, also the C/C genotype at this region was reported to be associated with a sustained response to HBV infection . However, in the Thai population, a higher frequency was reported of −511C/C genotype in the healthy controls in comparison to the patient with chronic hepatitis B .…”

Factors influencing PCV13 specific antibody response in Danish children starting in day care

“…Our study shows an increase in the buccal mucosal gene expressions of IL8 and IL1B in children that are carriers of any pneumococcal serotype in the nasal cavity at baseline even when adjusting for number of siblings as a potential risk factor for carriage of S. pneumoniae. Other studies have found a higher expression of IL8 and IL1B in serum in hepatitis B virus (HBV) infected patients [26][27][28] , while another study found mainly IL-10 to be expressed in HBV infected patients and HBV-vaccinated individuals 29 . Mucosal expression of IL8 and/or IL1B indicates activation of the mucosal immune system, which is expected in the presence of bacteria 30 .…”

Single Nucleotide Polymorphisms in IFN-γSignaling Pathway Associated with Risk of Hepatitis B Virus Infection in Chinese Children

“…Chronic HBV infection remains a serious global health problem [26,27]. It was reported that host genetic factors played an important role in the pathogenesis of HBV infection, and the polymorphisms of cytokine genes were strongly associated with the susceptibility to HBV infection in the general population [28,29]. ere is enough evidence that genetic variants of IFN-c signaling pathway genes play important roles in the HBV infection in adults [30][31][32].…”