Association of Interleukin 16 With the Development of Ovarian Tumor and Tumor-Associated Neoangiogenesis in Laying Hen Model of Spontaneous Ovarian Cancer

Yellapa, Aparna; Bahr, Janice M.; Bitterman, Pincas; Abramowicz, Jacques S.; Edassery, Seby L.; Penumatsa, Krishna; Basu, Sanjib; Rotmensch, Jacob; Barua, Animesh

doi:10.1097/igc.0b013e318236a27b

Cited by 33 publications

(36 citation statements)

References 22 publications

(33 reference statements)

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“…Immunohistochemical detection of markers of OVCA or ovarian tumor associated neoangiogenesis: Paraffin sections of normal or tumor ovaries were immunostained for the detection of IL-16 expressing cells and SMAexpressing microvessels using specific antibodies and the frequencies of the immunopositive cells and vessels were counted and analyzed as reported previously [12,13,5]. Differences in the frequency of these markers between normal and hens with OVCA were considered significant when the P < 0.05.…”

Section: Il-16-targeted Ultrasound Imagingmentioning

confidence: 99%

“…Compared with normal hens many IL-16 expressing cells were localized in hens with OVCA ( Figure 3B-C). (Figure 4, top panel) [5]. All standards and serum samples were run in duplicate.…”

Section: Detection Of Il-16 Expressing Cellsmentioning

confidence: 99%

“…IL-16 mRNA expression was assessed by semi-quantitative RT-PCR as reported previously [14,5]. Chicken-specific IL-16 primers were designed (Oligoperfect Designer software, Invitrogen, Carlsbad, CA) using IL-16 sequence from the NCBI (GeneBank: NM_204352.3).…”

Section: Il-16 Mrna Expression By Normal Ovaries or Ovaries With Tumomentioning

confidence: 99%

“…Anti-NMP antibodies are produced in response to shed NMPs [3,4]. On the other hand, expression of IL-16 by the tumor epithelium and its serum levels increase in association with ovarian tumor development [5]. Thus IL-16 represents a potential marker of early OVCA which can be detected by ultrasound imaging provided an IL-16-targeted MT-U/S imaging agent can be developed.…”

Section: Introductionmentioning

confidence: 99%

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Early Detection of Ovarian Cancer by Tumor Epithelium-Targeted Molecular Ultrasound

Barua¹

2013

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Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Department of Defense, Washington Headquarters Services, Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. REPORT DATE PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION REPORT NUMBERRush University Medical Center, Chicago, IL 60612 SPONSORING / MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR'S ACRONYM(S)U.S. Army Medical Research and Materiel Command Fort Detrick, Maryland 21702-5012 SPONSOR/MONITOR'S REPORT NUMBER(S) DISTRIBUTION / AVAILABILITY STATEMENTApproved for Public Release; Distribution Unlimited SUPPLEMENTARY NOTES ABSTRACTThe high rate of death of ovarian cancer (OVCA) patients can be prevented if it is detected at early stage. Unfortunately, currently available traditional transvaginal ultrasound (TVUS) imaging together with serum CA-125 levels cannot detect OVCA at early stage. Malignant nuclear transformations followed by the establishment of tumor associated neo-angiogenesis are the early events in tumor development. Ovulation is an inflammatory process which exposes ovarian surface and fimbrial epithelium to inflammatory factors including interleukin 16 (IL-16). Inflammation of the ovary and tubal epithelium due to frequent ovulation leads to the development of oxidative stress and longstanding unresolved oxidative stress causes malignant transformation. Expression of IL-16 by the tumor epithelium and its serum levels has been reported to be increased during OVCA development. Thus IL-16 represents a potential marker of early OVCA which can be detected in vivo by ultrasound imaging provided an IL-16-targeted molecular imaging agent can be developed. The goal of this study is to develop and test the efficacy of molecular (IL-16)-targeted ultrasound (MT-U/S) imaging probe for the detection of early OVCA. This goal is being accomplished by two specific aims. The results of Aim-1 suggest that IL-16-targeted imaging probes improved the visualization and detection of ovarian tumors in laying hens. These results will be used in Aim-2 to test the efficacy of IL-16-targeted MT-U/S imaging probes in detecting early OVCA in laying hens in a prospective study.

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Section: Il-16-targeted Ultrasound Imagingmentioning

confidence: 99%

“…Compared with normal hens many IL-16 expressing cells were localized in hens with OVCA ( Figure 3B-C). (Figure 4, top panel) [5]. All standards and serum samples were run in duplicate.…”

Section: Detection Of Il-16 Expressing Cellsmentioning

confidence: 99%

Section: Il-16 Mrna Expression By Normal Ovaries or Ovaries With Tumomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Early Detection of Ovarian Cancer by Tumor Epithelium-Targeted Molecular Ultrasound

Barua¹

2013

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“…Immunoassay and Immunoblotting: Serum prevalence of anti-NMP antibodies and IL-16 levels were determined by ELISA and confirmed by Western blotting as reported earlier [11,12].…”

Section: Gross Morphology and Histopathologymentioning

confidence: 99%

Early Detection of Ovarian Cancer by Molecular Targeted Ultrasound Imaging Together with Serum Markers of Tumor-Associated Nuclear Change and Angiogenesis

Barua¹

2013

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Regulation of Cellular Processes by Interleukin‐16 in Homeostasis and Cancer

Richmond

Tuzova

Cruikshank

2013

Journal Cellular Physiology

128

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Interleukin-16 (IL-16) is generated as a precursor molecule that is cleaved by caspase-3 to produce a pro-IL-16 molecule that functions as a regulator of T cell growth, and a secreted peptide that functions as a CD4 and/or CD9 ligand for induction of cell motility and activation. IL-16 has been predominantly studied as a contributing factor in the orchestration of an immune response; however, more recently IL-16 bioactivity has been closely associated with the progression of a number of different cancers. While the association between IL-16 plasma levels and tumor progression has been reported for many types of cancer, the mechanism for IL-16 involvement has been partially elucidated for three of the cancer types, cutaneous T cell lymphoma (CTCL), multiple myeloma (MM), and breast cancer. The mechanism for promoting cell growth is different in each of these cancers and involves a sequence mutation in the pro-molecule facilitating decreased p27(KIP1) levels in CTCL; over expression of the secreted IL-16 molecule to induce proliferation in CTCL T cells, and plasma cells in MM; and increased secreted IL-16 acting to recruit CD4+ pro-tumor macrophages in breast cancer. This article will review the cellular process for generating IL-16, the biological activities for both the pro- and secreted forms of the protein, and then the mechanism by which these forms contribute to cancer progression. As a soluble cytokine the ability to reduce or eliminate IL-16 synthesis through siRNA approaches or bioactivity through the use of neutralizing antibody treatment may represent a novel therapeutic approach.

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Association of Interleukin 16 With the Development of Ovarian Tumor and Tumor-Associated Neoangiogenesis in Laying Hen Model of Spontaneous Ovarian Cancer

Abstract: The results of the study suggest that changes in serum levels of IL-16 are associated with tumor development and TAN. Thus, serum IL-16 levels may be an indicator of ovarian TAN at the early stage of OVCA.

Cited by 33 publications

References 22 publications

Early Detection of Ovarian Cancer by Tumor Epithelium-Targeted Molecular Ultrasound

Early Detection of Ovarian Cancer by Tumor Epithelium-Targeted Molecular Ultrasound

Early Detection of Ovarian Cancer by Molecular Targeted Ultrasound Imaging Together with Serum Markers of Tumor-Associated Nuclear Change and Angiogenesis

Regulation of Cellular Processes by Interleukin‐16 in Homeostasis and Cancer

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