Association of immunolocalization of matrix metalloproteinase 1 with ovulation in hCG-treated rabbit ovary

Tadakuma, H.; Okamura, Hitoshi; Kitaoka, Mitsuhiko; Iyama, Ken‐ichi; Usuku, Gentaro

doi:10.1530/jrf.0.0980503

Cited by 46 publications

(34 citation statements)

References 11 publications

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“…Morphological observations indicate that preovulatory connective tissue disruption begins at the ovarian surface and advances inward to encompass the apical follicular wall [27,28]. Tunica/thecal fibroblasts and follicular steroidogenic (theca, granulosa) cells are sources of procollagenases [29,30]. General chemical inhibitors of collagenases suppressed ovulation (hamster, rat) in vitro and in vivo [31][32][33][34].…”

Section: Ovulationmentioning

confidence: 99%

Proteolytic Mechanisms in the Ovulatory Folliculo-Luteal Transformation

Murdoch

Gottsch²

2003

Connective Tissue Research

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The connective tissue matrix of the wall of ovarian follicles is degraded and remodeled during ovulatory rupture and formation of the corpus luteum. Ovarian surface epithelial cells in close contact with the apical wall of preovulatory ovine follicles secrete a urokinase-type plasminogen activator in response to surge levels of gonadotropins. Urokinase activates latent collagenases and stimulates release of tumor necrosis factor alpha from thecal endothelium. Tumor necrosis factor alpha progressively induces matrix metalloproteinase gene expression, apoptosis, and inflammatory necrosis. Collagenolysis and cellular death are a prelude to stigma formation and ovarian rupture. Ovulation is blocked by intrafollicular injection of TNFalpha or MMP-2 antisera. Unruptured follicles luteinize but are deficient in collagenous/vascularized trabeculae, and produce less progesterone than their control luteal counterparts. It appears that TNFalpha, via MMP-2 induction, contributes to the reorganization of an ovulatory follicle into a fully competent corpus luteum.

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Section: Ovulationmentioning

confidence: 99%

Proteolytic Mechanisms in the Ovulatory Folliculo-Luteal Transformation

Murdoch

Gottsch²

2003

Connective Tissue Research

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“…Tissue samples, frozen in liquid nitrogen, were cut at cryotome (Leica), in transverse cryosections of 5 µm, 10 section/organ, at -24°C. After that, the samples were air dried for [12][13][14][15][16] Histological and morphometric analyses were done by light microscopy (Olympus BX53), with compatible digital camera (Olympus UC50). Morphometric measurement of epithelial height was taken with computer-assisted image analysis system Olympus cellSens microscope imaging software, with magnification x40.…”

Section: Methodsmentioning

confidence: 99%

“…Immediate release of LH from the anterior pituitary reaches the maximum after 1 h, which results in ovulation [11] . After the ovulation in rabbits has been induced [1,12,13] , it starts 10-12 h later [14] , and until the corpora lutea are formed, estrogen is dominant ovarian hormone [15] . Epithelial lining of cervical mucosa is a simple columnar epithelium with ciliated cells and goblet cells.…”

Section: Introductionmentioning

confidence: 99%

Kaliforniya Tavşanının (Oryctolagus cuniculus) Farklı Üreme Dönemlerinde Uterustaki Histolojik ve İmmunolojik Değişiklikler

Milanovic¹,

Radovanović²,

Vasilijić³

et al. 2016

Kafkas Univ Vet Fak Derg

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Citation of This ArticleMilanovic V, Radovanovic A, Vasilijic S, Mrvic V, Milosevic B: Histological and immunological changes in uterus during the different reproductive stages at Californian rabbit (Oryctolagus cuniculus). Kafkas Univ Vet Fak Derg, 23, 137-144, 2017. DOI: 10.9775/kvfd.2016 Abstract Rabbit is the third most commonly used animal model in different fields of scientific research, such as reproductive biology, fertility and embryo transfer studies, and immunology. This animal species, often used in antibodies production, has minority of scientific records about the immunological status of its reproductive organs. The aim of this study was to find histological and immunological changes in rabbit female reproductive tract during different reproductive stages. The study was carried out on female rabbits, divided in three groups, according to the following stages of reproductive cycle: Estrous, ovulation and pregnancy. Histological and immunohistochemical stains for T-and B-cells were performed on tissue samples of cornu uteri and cervix. T lymphocytes were predominant in all anatomical parts of the uterus, in all stages of the cycle. The highest number of those cells was recorded at estrous, while the lowest was recorded at pregnancy. Cervix expressed more immunological activity than cornu uteri. The distribution and the number of immune positive cells in the rabbit female reproductive tract depend on its hormonal status. Keywords: Rabbit, Uterus, Cervix, B-cell, T-cells Kaliforniya Tavşanının (Oryctolagus cuniculus) Farklı Üreme Dönemlerinde Uterustaki Histolojik ve İmmunolojik Değişiklikler ÖzetTavşan; üreme biyolojisi, üretkenlik ve embriyo transferi çalışmaları ve immünoloji gibi farklı alanlardaki bilimsel çalışmalarda en sıklıkla kullanılan hayvan modelleri arasında üçüncü sırada yer almaktadır. Antikor üretiminde kullanılan bu hayvanların üreme organlarının immünolojik durumu hakkındaki raporlar oldukça kısıtlıdır. Bu çalışmanın amacı farklı üreme devrelerinde bulunan dişi tavşanların üreme organlarında şekillenen histolojik ve immünolojik değişiklikleri belirlemektir. Dişi tavşanlar östrus, ovulasyon ve gebelik olmak üzere üç gruba ayrıldı. Cornu uteri ve serviks dokularında histolojik incelemeler ile T ve B-hücreleri için immunohistokimyasal boyamalar yapıldı. T lenfositler uterusun tüm anatomik bölümlerinde ve tüm dönemlerde baskın hücre olarak belirlendi. Bu hücreler en yüksek oranda östrusta ve en düşük gebelikte tespit edildi. Servikste cornu uteriden daha fazla immünolojik aktivite belirlendi. Immun pozitif hücrelerin dağılım ve sayısının dişi tavşanların üreme organlarında hormon durumu ile ilişkili olduğu sonucuna varıldı.

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“…An accessory impetus of TNF-· on follicular collagenase biosynthesis is exerted at the transcriptional level [18]. Steroidogenic (granulosal/thecal) cells and fibroblasts are potential sources of collagenases [68,69]. An increase in expression of an inhibitor of metalloproteinases-1 within granulosal cells of preovulatory ovine follicles [70] probably serves to limit the extent of follicular destruction -ensuring that a viable corpus luteum can be formed.…”

Section: Up-regulation Of Collagenolysis By Plasmin and Tnf-·mentioning

confidence: 99%

Proteolytic and Cellular Death Mechanisms in Ovulatory Ovarian Rupture

Murdoch¹

2000

Biol Signals Recept

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Collagen breakdown and cellular death (apoptosis and inflammatory necrosis) within the apex of preovulatory ovine follicles are hallmarks of impending ovarian rupture. An integrative mechanism is presented whereby gonadotropic stimulation of urokinase-type plasminogen activator secretion by ovarian surface epithelial cells bordering the preovulatory follicle elicits a localized increase in tissue plasmin, which activates latent collagenases and secretion of tumor necrosis factor-α (TNF-α) from thecal endothelium. TNF-α potentiates collagenolysis (via matrix metalloproteinase gene expression) and (at elevated concentrations) mediates epithelial/vascular dissolution. Incidental damage to DNA of ovarian surface epithelial cells circumjacent to the ruptured follicle is a putative etiological factor in ovarian cancer.

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Association of immunolocalization of matrix metalloproteinase 1 with ovulation in hCG-treated rabbit ovary

Cited by 46 publications

References 11 publications

Proteolytic Mechanisms in the Ovulatory Folliculo-Luteal Transformation

Proteolytic Mechanisms in the Ovulatory Folliculo-Luteal Transformation

Kaliforniya Tavşanının (Oryctolagus cuniculus) Farklı Üreme Dönemlerinde Uterustaki Histolojik ve İmmunolojik Değişiklikler

Proteolytic and Cellular Death Mechanisms in Ovulatory Ovarian Rupture

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