Association of IL33/ST2 signal pathway gene polymorphisms with myocardial infarction in a Chinese Han population

Abstract

This study investigated the relationship between IL-33/ST2 signal pathway gene polymorphisms and myocardial infarction (MI) in Han Chinese. A case-control association analysis was performed on a total of 490 MI patients (MI group) and 929 normal subjects (NC group). Sequenom Mass Array and Taqman genotyping technique were used to analyze the tag single nucleotide polymorphisms (SNPs) in the genes encoding IL-33, ST2, and IL-1RaP (rs11792633, rs1041973 and rs4624606). The results showed that the frequencies of rs4624606 genotypes AA, TT, AT were 0.031, 0.647, 0.322 in MI group and 0.026, 0.712, 0.263 in NC group, and the allele frequencies of A and T were 0.192, 0.808 in MI group and 0.157, 0.843 in NC group. There were significant differences in rs4624606 genotypes and allele frequencies between MI group and NC group (P<0.05). For rs11792633, the allele frequencies of C and T were 0.45, 0.55 in MI group and 0.454, 0.546 in NC group with no significant differences found between the two groups. Compared with genotype CC+TC, rs11792633 genotype TT had an increased risk of hypertension (P<0.05). However, there were no significant differences in the frequencies of rs11792633 genotypes between the two groups. No significant differences were noted in the frequencies of rs1041973 genotype and allele between the two groups. Logistic regression analysis showed that rs4624606 genotypes AT and AA+AT were both significantly associated with MI (AT: OR=1.325, P=0.029, 95% CI=1.03-1.705; AA+AT: OR=1.316, P=0.028, 95% CI=1.03-1.681) after factors such as age, gender, smoking, drinking, body mass index (BMI), triglyceride (TG) and cholesterol were adjusted. Those carrying rs4624606 genotype AT or AA+AT had an increased risk of MI. No associations were found between the polymorphisms of the other two loci with MI. It was concluded that, in the IL33/ST2 signal pathway, the A allele of rs4624606 polymorphism of IL-1RaP gene is a potential independent risk factor for MI, and the genotypes AA+AT and AT are associated with the incidence of MI.

The IL-33/ST2 pathway, inflammation and atherosclerosis: Trigger and target?

“…When assessing 1146 CAD patients and age-and sex-matched controls, no significant association between gene polymorphisms in the IL-33 or ST2 genes and presence/absence of CAD was demonstrated [28]. Similar results were found when comparing patients with previous MI vs. control subjects [29]. Notably, the relevance of these analyses is limited by the small number of gene variants explored, and by the lack of genotype-phenotype correlations, making it difficult to clarify the relationship with ST2/IL-33 signalling.…”

Case-control study on IL-1RAcP rs16865597 gene polymorphism and susceptibility to essential hypertension in a Chinese Han population

“…Soluble IL-1RAcP interacts with the sST2-IL-33 complex to impair IL-33 signaling [23]. Previous studies indicated the importance of IL-1RAcP in cardiovascular diseases, such as myocardial infarction and EH [24,25]. Nikpay and coworkers found that the IL-1RAcP polymorphism rs4687150 is associated with the risk of EH 25 .…”

IL-33/ST2 immunobiology in coronary artery disease: A systematic review and meta-analysis

“…Studies that did not meet these standards were excluded. Thus, seven casecontrol studies met our inclusion criteria for qualitative data analysis, and contained a total of 10686 cases and 10775 healthy subjects (7,8,(37)(38)(39)(40)(41). Two researchers simultaneously screened the studies back-to-back and obtained consistent results.…”