Association of IL-6 rs1800796 Gene Polymorphism on Susceptibility to Pulmonary Tuberculosis

Abstract

Introduction: Tuberculosis (TB) is still the leading cause of mortality and morbidity around the globe, particularly in underdeveloped nations. Indonesia is listed as the country with the second-largest TB burden in the world according to Global TB Report 2020. Only 10% of those infected with Mycobacterium tuberculosis (MTB) develop active TB and the great majority continues to stay latently infected, indicating that the host response to MTB may be genetically controlled at each stage. A growing number of studies have shown that numerous host genetic variables have a role in TB susceptibility. IL-6 is a pro-inflammatory cytokine produced by monocytes, endothelial cells, and fibroblasts that stimulates B and T lymphocytes while also controlling the immune response to TB. This study investigated the association of IL-6 rs1800796 gene polymorphism on susceptibility to pulmonary TB in drug-sensitive (DS) or drug-resistant (DR) patients. Methods: The case-control study involved 71 cases and 34 controls. Blood samples were taken from all study participants at Dr. Saiful Anwar General Hospital, Malang between August 2020 and February 2021. To determine SNPs, blood samples were used in a genotyping investigation using the PCR-Multiplex technique. Results: The genotype association of IL-6 rs1800796 (G/G, G/C, C/C) and allele frequency for patients and controls of IL-6 rs1800796 with susceptibility to pulmonary TB were insignificant (p > 0.05). Conclusion: This study found no evidence of association of IL-6 rs1800796 gene polymorphism with susceptibility to pulmonary TB.