Association of GSTT1 null, XPD 751 CC and XPC 939 CC genotypes with increased levels of genomic damage among hospital pathologists

Santovito, Alfredo; Delsoglio, Marta; Manitta, Eleonora; Picco, Giulia; Meschiati, Giulia; Chiarizio, Michela; Gendusa, Claudio; Cervella, Piero

doi:10.1080/1354750x.2017.1322147

Cited by 8 publications

(7 citation statements)

References 54 publications

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“…Therefore, XPD serves an important role in the TFIIH complex-mediated NER and its transcription process (3). In addition, XPD participates in various physiological and pathological processes, such as cellular proliferation, apoptosis and tumorigenesis (13,(15)(16)(17)(18)(19)(20)(21)(22). In the present study, the XPD gene was cloned into a eukaryotic expression vector to construct the pEGFP-N1/XPD recombinant plasmid; this was confirmed by sequencing, which was consistent with the XPD sequence published on GenBank.…”

Section: Discussionsupporting

confidence: 76%

Cloning of the XPD gene and its function in malignant melanoma cells

et al. 2020

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The xeroderma pigmentosum group D (XPD) gene is a member of the transcription factor IIH complex and serves an important role in gene repair. Previous studies have suggested that genetic variants of the XPD gene may be associated with an increased risk of cutaneous melanoma. However, the exact mechanism remains unclear. In the present study, the XPD gene was cloned, and its localization and function in malignant melanoma cells were investigated. The human full length XPD gene was cloned via reverse transcription-PCR using the total RNA extracted from human cervical squamous cell carcinoma epithelial HeLa cells. Subsequently, the gene was inserted into a plasmid fused to green fluorescent protein (GFP; pEGFP-N1/XPD), and pEGFP-N1/XPD and pcDNA3.1(+)/XPD were transfected into human malignant melanoma A375 cells using Lipofectamine ® 2000. The expression levels of XPD were detected by western blotting. The Golgi marker GM130 and the endoplasmic reticulum membrane protein marker KDEL were used for immunofluorescence staining, and the subcellular localization of XPD was observed under a fluorescence microscope. Cell proliferation was measured using an MTT assay. The recombinant pEGFP-N1/XPD plasmid expressing the human wild-type XPD gene was successfully constructed by restriction enzyme digestion and assessed by gene sequencing. XPD was localized in the endoplasmic reticulum of malignant melanoma A375 cells, as confirmed by immunofluorescence staining. Furthermore, MTT assays indicated that XPD inhibited the proliferation of malignant melanoma A375 cells. The present study provides a basis for further investigation of the biological effects and functions of XPD in malignant melanoma cells.

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Section: Discussionsupporting

confidence: 76%

Cloning of the XPD gene and its function in malignant melanoma cells

et al. 2020

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“…Specifically, the Ile462Val polymorphism, causing a substitution in the enzyme heme-binding region, result in a twofold increase of the microsomal enzyme activity and is a risk factor for many types of cancer and hematopoietic malignancies, such as acute leukaemia (Zhuo et al 2012;Roszak et al 2014). However, previous studies did not report any association between this polymorphism and SCEs frequency (Carere et al 2002;Santovito et al 2017). The human XPC (Xeroderma pigmentosum complementation group C) gene encodes for a 940-amino acid protein essential within the NER (Nucleotide Excision Repair) pathway, involved in the early damage site recognition and DNA repair initiation (Dai et al 2019).…”

Section: Discussionmentioning

confidence: 99%

“…The determination of the SCE/cell number for each subject was performed scoring 50 well-spread second-division metaphases containing 46 chromosomes. The replication index (RI) evaluation was performed scoring a total of 100 cells from each donor and calculated according to the following formula: RI = (M1 + 2 M2 + 3 M3)/N, where M1, M2 and M3 represent the number of cells undergoing first second and third mitosis and N is the total number of scored metaphases (NSM) (Santovito et al 2014(Santovito et al , 2017.…”

Section: Blood Sampling and Sces Assaymentioning

confidence: 99%

The formation of SCEs as an effect of occupational exposure to formaldehyde

et al. 2022

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Formaldehyde (FA) is a ubiquitous toxic chemical employed worldwide due to its disinfectant and preservative properties. Despite being classified as a human carcinogen, FA is still employed as formalin in pathology wards as standard fixative. We evaluated its relationship with the formation of sister-chromatid exchanges (SCEs) in cultured peripheral blood lymphocytes on 57 pathologists and 48 controls and the risk/protective role played by several genetic polymorphisms. All subjects were assessed for SCEs and genotyped for the most common cancer-associated gene polymorphisms: CYP1A1 exon 7 (A > G), CYP1A1*2A (T > C), CYP2C19*2 (G > A), GSTT1 (presence/absence), GSTM1 (presence/absence), GSTP1 (A > G), XRCC1 (G399A), XRCC1 (C194T), XRCC1 (A280G), XPC exon 15 (A939C), XPC exon 9 (C499T), TNFα − 308 G > A), IL10 − 1082 (G > A), and IL6 − 174 (G > C). Air-FA concentration was assessed through passive personal samplers. Pathologists, exposed to 55.2 μg/m3 of air-FA, showed a significantly higher SCEs frequency than controls, exposed, respectively, to 18.4 μg/m3. Air-FA was directly correlated with SCEs frequency and inversely with the replication index (RI). Regression models showed FA exposure as a significant predictor in developing SCEs, while did not highlight any role of the selected polymorphisms. Our study confirms the role of low air-FA levels as genotoxicity inductor, highlighting the importance to define exposure limits that could be safer for exposed workers.

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“…In order to evaluate the possible influence of the sex on the level of genomic damage, age and sex data was collected. It is well known that drugs and X-rays can alter the level of genomic damage (Santovito et al 2017). Therefore, we excluded subjects that had contracted acute infections and/or chronic non-infectious diseases and exposure to diagnostic X-rays for a minimum of two years prior to the analysis.…”

Section: Subjectsmentioning

confidence: 99%

Evaluation of Micronuclei Frequency in Both Shelter and Family Cats and Dogs

Santovito

Buglisi

Scarfò

2020

Preprint

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Each year a lot of animals are cared for in shelters in Italy. Many of these animals have received minimal or no prior healthcare. Thus, the beneficial role animal shelters play is undeniable. Nonetheless, even well-run shelters lack the necessary resources to provide adequate conditions. It is common knowledge that group-housing can increase stress levels in family cats (Felis silvestris lybica) and dogs (Canis lupus familiaris) contributing to the development of infectious diseases and/or behavioural problems.The aim of this study is to evaluate, through the buccal micronucleus assay, the level of genomic damage in shelter cats and dogs and compare it with that of family cats and dogs. The hypothesis is that environmental conditions such as those potentially present in shelters can affect the level of genomic damage.The study population included thirty healthy mixed breed cats and dogs, randomly sampled, with at a minimum two-year presence in a shelter. The control group consisted of thirty healthy cats and dogs living in a home environment, using age/sex matching. The micronucleus assay was performed on one thousand exfoliated buccal mucosa cells per subject and standardized protocols were used for stress score tests.Significant differences were found between shelter and family cats and dogs in terms of micronuclei, indicating that a condition of stress found in sheltered cats may increase the levels of genomic damage. Conversely, no significant differences in the frequency of micronuclei were found between the sexes, as well as no correlation was found between age and the frequencies of the used genomic markers.

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Association of GSTT1 null, XPD 751 CC and XPC 939 CC genotypes with increased levels of genomic damage among hospital pathologists

Cited by 8 publications

References 54 publications

Cloning of the XPD gene and its function in malignant melanoma cells

Cloning of the XPD gene and its function in malignant melanoma cells

The formation of SCEs as an effect of occupational exposure to formaldehyde

Evaluation of Micronuclei Frequency in Both Shelter and Family Cats and Dogs

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