Association ofDGAT2gene polymorphisms with carcass and meat quality traits in domestic pigeons(Columba livia)

Abstract

1. Diacylglycerol acyltransferase (DGAT) plays an important role in the synthesis of triacylglycerol, but its effects on meat quality and carcass composition in pigeons are unclear. In this study, single-nucleotide polymorphisms (SNPs) in the exons of the DGAT2 gene were identified and analysed by using DNA sequencing methods in 200 domestic pigeons (Columba livia). The associations between DGAT2 polymorphisms and carcass and meat quality traits were also analysed. 2. Sequencing results showed that 5 nucleotide mutations were detected in exons 3, 4, 5 and 6 of the DGAT2 gene. The analysis revealed three genotypes (AA, AB and BB) in G18398T and G22484C, in which the AA genotype and A allele had the highest frequency. 3. In the SNP of G18398T located in exon 5, individuals with genotype BB had significantly higher meat quality and lower abdominal fat content than those with AA or AB genotype. In the SNP of G22484C located in exon 6, the genotype AA showed highest carcass trait values, while the genotype BB represented better meat quality, compared to AA and AB genotypes. 4. The results imply that DGAT2 gene has a close relationship with carcass and meat quality traits in pigeons, and the SNPs of G18398T and G22484C can be used as genetic markers for marker-assisted breeding in pigeon.

Tissue Expression and Variation of the DGAT2 Gene and Its Effect on Carcass and Meat Quality Traits in Yak

“…Yin et al [15] and Li et al [16] reported that a SNP and 13 bp indel in the 3′-UTR of DGAT2 were associated with backfat thickness and lean percentage in pigs. Mao et al [18] found that SNPs in exons 5 and 6 of DGAT2 had an effect on carcass and meat quality traits in domestic pigeons. In goats, variations in exon 3 of DGAT2 were found to be associated with growth traits [35], while variations in exon 4 and intron 5 had an influence on the milk yield and fat percentage [17].…”

“…The expression of DGAT2 is positively correlated to intramuscular fat (IMF) content in longissimus dorsi muscles of Korean steers [13] and pigs [14]. Variation in DGAT2 has been reported to affect carcass muscle and milk quality traits in various animals, such as fat yield and retail cut weight percentage in commercial feedlot steers [11], backfat thickness and lean percentage in pigs [15,16], milk yield and fat percentage in goats [17], and carcass weight, shear force, and IMF in domestic pigeons [18].…”

Comparative Transcriptome Profiling of mRNA and lncRNA of Ovaries in High and Low Egg Production Performance in Domestic Pigeons (Columba livia)

“…Thus, poultry breeders have focused on the ovaries to study egg production. Moreover, the domestic pigeon is one of the most economically important poultry species in China, as they are a source of meat and eggs, and China is also the largest consumer market for pigeon eggs (Mao et al, 2018). Therefore, it is of great significance to study the regulatory mechanisms of reproduction in ovaries of the pigeon to improve their egg production performance.…”

Identification, molecular characteristics, and tissue differential expression of <i>DGAT2</i> full-CDS cDNA sequence in Binglangjiang buffalo (<i>Bubalus bubalis</i>)

“…Bovine DGAT2 has been mapped to BTA15q25-26 by fluorescence in situ hybridization (FISH) (Winter et al, 2003). Studies have shown that the polymorphisms of DGAT2 gene were associated with economic traits in some domestic animals, such as carcass, meat quality, and fat yield traits in commercial feedlot steers (Li et al, 2009); back-fat thickness in pigs (Yin et al, 2012); carcass weight and shear force in domestic pigeons (Mao et al, 2018); body weight and length in cattle (Zhang et al, 2007;Mao et al, 2008); and milk yield and fat percentage in goats (An et al, 2011).…”