Association of <i>ADSL</i> gene polymorphisms with meat quality and carcass traits in domestic pigeons (<i>Columba livia</i>)

Mao, Haoyu; Cao, Haiyue; Liu, H H; Dong, Xin; Xu, N.; Yin, Zhaozheng

doi:10.1080/00071668.2018.1493188

Cited by 10 publications

(9 citation statements)

References 19 publications

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“…With improving living standards, the demands for poultry meat are not only concentrated on carcass composition but also meat quality (2), especially in China, where people prefer to cook poultry meat by steaming and souping. Domestic pigeons are wildly raised in southern China as a kind of meat-type poultry for the abundant nutrients and delicious taste (3). Numerous studies have been carried out on other poultry, such as chickens and ducks, to identify genes regulating IMF content and muscle fiber characteristics (4,5), but studies on domestic pigeons are still limited.…”

Section: Introductionmentioning

confidence: 99%

Expression of DGAT2 Gene and Its Associations With Intramuscular Fat Content and Breast Muscle Fiber Characteristics in Domestic Pigeons (Columba livia)

et al. 2022

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Diacylglycerol acyltransferase 2 (DGAT2) catalyzes the final step in triglyceride synthesis and plays an important role in the synthesis of fat, but the effects of its expression on intramuscular fat (IMF) content and muscle development are still unknown. In this study, we investigated the expression of the DGAT2 gene and its associations with IMF content and breast muscle fiber characteristics in pigeons. The spatiotemporal expression profile of the pigeon DGAT2 gene in breast muscle showed that the mRNA expression level of DGAT2 gene in subcutaneous fat was the highest (p < 0.01) among eight tissues from 0 to 4 weeks of age, and showed an upward trend week by week, followed by liver (p < 0.05). Moreover, both mRNA and protein levels of the DGAT2 gene in breast muscle showed an upward trend from 0 to 4 weeks (p < 0.05), accompanied by the upregulation of MYOD1 and MSTN. In addition, the paraffin section analysis results revealed that the diameter and cross-sectional area of pectoralis muscle fiber significantly increased with age (p < 0.05), and a significant positive correlation was shown between the DGAT2 gene expression level and muscle fiber diameter (p < 0.05). Furthermore, correlation analysis suggested that the mRNA expression level of the pigeon DGAT2 gene was significantly (p < 0.01) correlated with IMF content in breast muscle. These results imply that the DGAT2 gene has a close relationship with IMF content and breast muscle fiber characteristics in pigeons, indicating that the DGAT2 gene might be used as a candidate gene marker-assisted breeding in pigeons.

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Section: Introductionmentioning

confidence: 99%

Expression of DGAT2 Gene and Its Associations With Intramuscular Fat Content and Breast Muscle Fiber Characteristics in Domestic Pigeons (Columba livia)

et al. 2022

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“…Such approach was applied in the analysis of polymorphisms in adenylosuccinate lyase (ADSL) gene. Mao et al (2018) amplified and sequenced exons of ADSL to find polymorphisms associated with meat quality and carcass traits in domestic pigeons. Researchers found nine polymorphic nucleotides, and two of them (located in exons 10 and 11) displayed a significant association with a higher carcass rate.…”

Section: Real Time-pcr and High Resolution Melting (Hrm)mentioning

confidence: 99%

Meat quality - Genetic background and methods of its analysis

Kowalczyk¹,

Kaliniak-Dziura²,

Prasow³

et al. 2022

Czech J. Food Sci.

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Growing consumer awareness is forcing food producers to supply raw material and products of increasingly high quality and health-promoting properties. Knowledge of the genetic background of quality characteristics is taking on great importance, enabling selection based on molecular markers. The increasing throughput of molecular techniques, in combination with an expanding bioinformatics infrastructure, is leading to continual improvement in understanding of the molecular mechanisms influencing meat quality. This has resulted in the identification of polymorphic nucleotides [single nucleotide polymorphisms (SNPs)] showing a relationship with meat characteristics such as tenderness [polymorphism in the calpain (CAPN) and calpastatin (CAST) genes], marbling [diacylglycerol o-acyltransferase 1 (DGAT1)], colour, pH and water-holding capacity (WHC) [CAST, stearoyl-CoA desaturase (SCD) and others], and fatty acid profile (SCD1). An increasingly wide range of methods is used for analysis, from techniques based on amplification of nucleic acids [polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation system-PCR (ARMS-PCR)] through Sanger sequencing to high-throughput next-generation sequencing (NGS) techniques. This paper is a review of the literature on polymorphism of genes determining the quality characteristics of meat and molecular methods used to detect them.

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“…Besides health management programs, including the appropriate choice of feeds, medical supplements, and physical conditioning regimens to improve pigeons’ performance in competitions, the identification of genetic markers for valuable traits associated with racing performance has also been reported and explored towards expediting success in selective breeding [ 1 , 6 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ]. The determination of single nucleotide polymorphisms (SNPs) that seem to cause phenotypic differences is one of the most commonly used approaches to identify potentially essential genetic markers [ 20 , 21 , 22 , 23 ]. Genotyping based on SNPs may be executed through several methods, among which polymerase chain reaction—restriction fragment length polymorphism (PCR-RFLP) is the most commonly performed.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of KASP Assays for the Genotyping of Racing Performance-Associated Single Nucleotide Polymorphisms in Pigeons

Chang

Silva

Huang

et al. 2021

Genes

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Pigeon racing’s recent upturn in popularity can be attributed in part to the huge prize money involved in these competitions. As such, methods to select pigeons with desirable genetic characteristics for racing or for selective breeding have also been gaining more interest. Polymerase chain reaction—restriction fragment length polymorphism (PCR-RFLP) for genotyping-specific genes is one of the most commonly used molecular techniques, which can be costly, laborious and time consuming. The present study reports the development of an alternative genotyping method that employs Kompetitive Allele Specific Polymerase Chain Reaction (KASP) technology with specifically designed primers to detect previously reported racing performance-associated polymorphisms within the LDHA, MTYCB, and DRD4 genes. To validate, KASP assays and PCR-RFLP assays results from 107 samples genotyped for each of the genes were compared and the results showed perfect (100%) agreement of both methods. The developed KASP assays present an alternative rapid, reliable, and cost-effective method to identify polymorphisms in pigeons.

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Association of ADSL gene polymorphisms with meat quality and carcass traits in domestic pigeons (Columba livia)

Cited by 10 publications

References 19 publications

Expression of DGAT2 Gene and Its Associations With Intramuscular Fat Content and Breast Muscle Fiber Characteristics in Domestic Pigeons (Columba livia)

Expression of DGAT2 Gene and Its Associations With Intramuscular Fat Content and Breast Muscle Fiber Characteristics in Domestic Pigeons (Columba livia)

Meat quality - Genetic background and methods of its analysis

Development and Validation of KASP Assays for the Genotyping of Racing Performance-Associated Single Nucleotide Polymorphisms in Pigeons

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