Association of HSV-1 and Reduced Oral Bacteriota Diversity with Chemotherapy-Induced Oral Mucositis in Patients Undergoing Autologous Hematopoietic Stem Cell Transplantation

Lee, Ahreum; Hong, Junshik; Shin, Dong Yeop; Koh, Youngil; Yoon, Sung Soo; Kim, Pil Jong; Kim, Hong Gee; Kim, Inho; Park, Hee Kyung; Choi, Youngnim

doi:10.3390/jcm9041090

Cited by 23 publications

(39 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The overall diversity of the oral microbiota decreased after chemotherapy, and the bacterial composition changed after the start of chemotherapy. A decrease in the diversity of the oral microbiota due to chemotherapy for hematopoietic malignancies has been reported in children with leukemia [20] and in patients undergoing autologous hematopoietic stem cell transplantation [21], which is consistent with the results of our study. Furthermore, recent reports have shown that changes in the diversity of not only the gut microbiota [22], but also the oral microbiota, during chemotherapy for leukemia are associated with the risk of infection during treatment [23,24].…”

Section: Discussionsupporting

confidence: 93%

Changes in oral microbiota after the initiation of chemotherapy in patients with hematopoietic tumors

Omori

Kato-Kogoe

Sakaguchi

et al. 2021

Preprint

View full text Add to dashboard Cite

Background Recently, the gut microbiota has been shown to play an important role in the response and resistance to chemotherapy. Although there is much knowledge about chemotherapy-induced changes in the gut microbiota, chemotherapy-associated changes in the oral microbiota remain unclear. Herein, we aimed to evaluate the changes in oral microbiota associated with the initiation of chemotherapy in patients with malignant hematopoietic tumors. Methods Oral samples were collected before and 8–20 days after the start of chemotherapy from 50 patients with malignant hematopoietic tumors who were starting chemotherapy for the first time. The 16S ribosomal RNA gene sequencing of bacterial DNA extracted from oral samples was performed to compare the oral microbiota before and after the initiation of chemotherapy. Results The richness or evenness of diversity in the ‘after start of chemotherapy’ group decreased significantly, compared with the ‘before start of chemotherapy’ group (alpha-diversity; observed operational taxonomic units (OTUs) index, p < 0.001; and Shannon’s index, p < 0.001). The overall salivary microbiota structure between the pre- and post-chemotherapy groups differed significantly (beta-diversity; unweighted UniFrac distances, p = 0.001; and weighted UniFrac distances, p = 0.003). Linear discriminant analysis effect size analysis demonstrated an increased abundance of species of certain genera, such as Staphylococcus, and decreased abundance of species of some genera, such as Streptococcus and Neisseria, in the ‘after-chemotherapy’ group, compared with those in the ‘before-chemotherapy’ group. The amounts and trends of change in the oral microbiota before and after the start of chemotherapy differed among the subjects. Of the 25 bacterial genera whose prevalence changed significantly before and after the start of chemotherapy, the proportion of oral commensals such as Streptococcus and Neisseria decreased in many subjects. In contrast, Staphylococcus and Pseudomonas were detected only in a few subjects, but their relative abundance increased significantly after the start of chemotherapy. Conclusions The oral microbiota of patients with hematopoietic tumors changed markedly after the initiation of chemotherapy. Our findings are expected to aid the elucidation of the pathogenesis of oral mucositis, which is an adverse event of chemotherapy, and the development of treatment methods for this condition.

show abstract

Section: Discussionsupporting

confidence: 93%

Changes in oral microbiota after the initiation of chemotherapy in patients with hematopoietic tumors

Omori

Kato-Kogoe

Sakaguchi

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…In our study, there was no association between oral shedding of herpesviruses and type of HSCT (allogeneic versus autologous). However, the severity of OM was associated with HSV‐1 reactivation and decrease in oral bacterial diversity (Lee et al., 2020), showing the influence of the imbalance on the local microenvironment.…”

Section: Discussionmentioning

confidence: 99%

Oral shedding of CMV and HSV‐1 in hematopoietic stem cell transplantation patients

et al. 2020

View full text Add to dashboard Cite

Objectives To evaluate the oral shedding of herpesviruses in patients undergoing hematopoietic stem cell transplantation (HSCT) and correlate it with oral mucositis (OM). Methods Saliva samples were collected before the HSCT and on day D + 8. Multiplex Polymerse Chain Reaction (PCR) was performed to detect herpes simplex virus (HSV)‐1 and HSV‐2, Epstein‐Barr virus (EBV), Cytomegalovirus (CMV), Variella‐zoster virus (VZV), and human herpesvirus (HHV)‐6, HHV‐7, and HHV‐8. OM was assessed according to WHO criteria. Results Thirty one patients were enrolled, in which 20 of 31 (64.5%) were males; median age was 50 (21–70) years; 16 of 31 (51.6%) underwent allo‐HSCT; and 15 of 31 (48.4%) underwent auto‐HSCT. On D + 8, OM grades III and IV were observed in 8 of 31 (25.8%) patients. In the first salivary collection, EBV was found in 24 of 31 (77.4%), followed by HHV‐6 (7/31, 22.6%) and HHV‐7 (8/31 25.8%). In the second collection, EBV was found in 24 of 27(89%), followed by HSV‐1 (8/27, 30%) and CMV, HHV‐6, and HHV‐7 (5/27, 18.5%, each one). On D + 8, OM grades II and IV were associated with the presence of HSV‐1. HSV‐1 was also associated with worsening degrees of OM on D + 15. Conclusion The presence of HSV‐1 and CMV in oral samples was more frequent on day D + 8 after HSCT. HSV‐1 detection was associated with severity and worsening of OM. HSV‐1 and CMV seem to be associated with oral dysbiosis due to HSCT.

show abstract

“…Herpes simplex virus type-1 (HSV-1) is a virus transferred usually by oral-to-oral contact, while HSV-2 is transferred mainly sexually. In the study of Lee, chemotherapy was associated with reduced diversity of oral bacterial microbiota and increased occurrence of HSV-1 infection in patients undergoing auto-HSCT [4]. Interestingly, HSV-1 infection reduced microbial diversity after treatment with chemotherapy.…”

Section: Herpesviridae (Hsv)mentioning

confidence: 97%

“…Nowadays, due to widespread different environmental factors, inadequate foods, use of antimicrobial drugs influencing the microbiome, the bacterial community can be disrupted [2,3]. Human microbiota colonizing the oral mucosa, intestinal tract, genital tract, and airways plays an important role in the human body and influences the development of chronic diseases such as alcoholic liver disease, autoimmune liver disease, or cancers [2,4,5]. It has been evidenced that the intestinal microbiome can be involved in inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), type 2 diabetes (D2M), and colorectal cancer [2,6].…”

Section: Introduction and Purposementioning

confidence: 99%

The role of microbiota in pathogenesis and development of viral infections

Maksymowicz

Ręka²,

Machowiec³

et al. 2021

J Educ Health Sport

View full text Add to dashboard Cite

Introduction and purpose The microbiota plays an important role in human metabolism, immune system, and development of many chronic diseases, cancers, and infectious diseases. The aim of the study is to present the role of gut microbiota in viral infections, including HBV, Herpesviridae, HIV, and SARS-CoV-2. The newest publications from the last 5 years available on the PubMed database were taken into account. A brief description of the state of knowledge The mechanism by which bacterias have an impact on viral infection is based on a synthesis of specific short-chain fatty acids (SCFAs) and modulation of cytokine release and immune system function by bacterias. Domination of Gardnerella vaginalis and lack of Lactobacillus in the vaginal microbiome increased the risk of HSV-2 infection in women. Inflammation of the genital tract can influence susceptibility to HIV infection, but probiotics via enhancement of the gut barrier integrity, change TH17/Treg ratio, can restore microbiome composition. LPS - component of the structure of Gram-negative bacteria can be a marker of HBV infection. In the airway microbiome of patients with COVID-19 opportunistic microorganisms were identified. Conclusions Diet, intake of probiotics, fecal microbiota transplantation (FMT) are interventions that might be efficient methods in prophylaxis and treatment of viral diseases. Further studies are needed to evaluate the mechanism of action of microbiome in pathogenesis of infectious diseases.

show abstract

Association of HSV-1 and Reduced Oral Bacteriota Diversity with Chemotherapy-Induced Oral Mucositis in Patients Undergoing Autologous Hematopoietic Stem Cell Transplantation

Cited by 23 publications

References 31 publications

Changes in oral microbiota after the initiation of chemotherapy in patients with hematopoietic tumors

Changes in oral microbiota after the initiation of chemotherapy in patients with hematopoietic tumors

Oral shedding of CMV and HSV‐1 in hematopoietic stem cell transplantation patients

The role of microbiota in pathogenesis and development of viral infections

Contact Info

Product

Resources

About