Association of Helicobacter pylori cagA Gene with Gastric Cancer and Peptic Ulcer in Saudi Patients

Saber, Taisir; Ghonaim, Mabrouk; Yousef, A. A.; Khalifa, Amany Salah; Qurashi, Hesham Al; Shaqhan, Mohammad; Samaha, M. A.

doi:10.4014/jmb.1501.01099

Cited by 18 publications

(11 citation statements)

References 47 publications

(72 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the received ORs for CagA and VacA m1 in the atrophic group were in the same range as reported by Gao et al [ 30 ], but in contrast to Gao et al [ 30 ], we could not observe significant ORs for the seroresponse to antigen HP0231. Furthermore, CagA seropositivity could also be associated with ulcer (OR = 2.5) as observed in our study, which is in accordance with previous studies [ 31 , 32 ], but the 95% CI did not show a significance. However, it has to be mentioned that high variability of the S/CO values in the different groups was observed, and due to small sample sets, 95% CIs were very broad.…”

Section: Discussionsupporting

confidence: 92%

Performance of a Multiplex Serological Helicobacter pylori Assay on a Novel Microfluidic Assay Platform

et al. 2017

View full text Add to dashboard Cite

Infection with Helicobacter pylori (H. pylori) occurs in 50% of the world population, and is associated with the development of ulcer and gastric cancer. Serological diagnostic tests indicate an H. pylori infection by detecting antibodies directed against H. pylori proteins. In addition to line blots, multiplex assay platforms provide smart solutions for the simultaneous analysis of antibody responses towards several H. pylori proteins. We used seven H. pylori proteins (FliD, gGT, GroEL, HpaA, CagA, VacA, and HP0231) and an H. pylori lysate for the development of a multiplex serological assay on a novel microfluidic platform. The reaction limited binding regime in the microfluidic channels allows for a short incubation time of 35 min. The developed assay showed very high sensitivity (99%) and specificity (100%). Besides sensitivity and specificity, the technical validation (intra-assay CV = 3.7 ± 1.2% and inter-assay CV = 5.5 ± 1.2%) demonstrates that our assay is also a robust tool for the analysis of the H. pylori-specific antibody response. The integration of the virulence factors CagA and VacA allow for the assessment of the risk for gastric cancer development. The short assay time and the performance of the platform shows the potential for implementation of such assays in a clinical setting.

show abstract

Section: Discussionsupporting

confidence: 92%

Performance of a Multiplex Serological Helicobacter pylori Assay on a Novel Microfluidic Assay Platform

et al. 2017

View full text Add to dashboard Cite

show abstract

“…When compared to histology, the relative sensitivity of serology, RUT, and culture was 90%, 81%, and 63%, respectively, while their relative specificity was 47%, 92%, and 93%, respectively. In addition, Saber et al reported that compared to PCR, the relative specificity and sensitivity of an anti-cagA IgG serologic test were 89.6% and 91.6%, respectively [81]. Serologic tests using serum and salivary were also considered in a study conducted in Abha, Saudi Arabia, by El-Mekki et al [82].…”

Section: Guidelines For the Diagnosis Of H Pylori Infectionmentioning

confidence: 99%

Prevalence of Helicobacter pylori Infection and Diagnostic Methods in the Middle East and North Africa Region

et al. 2020

View full text Add to dashboard Cite

Background and Objectives: Helicobacter pylori (H. pylori) infection is common worldwide and may cause gastroduodenal complications, including cancer. In this review, we examine the prevalence and distribution of various H. pylori genotypes and the risk factors for H. pylori infection, particularly in the Middle East and North Africa (MENA) region. We also introduce different global screening methods and guidelines and compare them to those currently in use in the MENA region. Materials and Methods: We searched the Google Scholar, PubMed, and Saudi Digital Library (SDL) databases for clinical trials and articles published in English. The data collection was mainly focused on MENA countries. However, for H. pylori genotypes and diagnostic methods, studies conducted in other regions or reporting global practices and guidelines were also included to allow a comparison with those in the MENA region. We also included studies examining the prevalence of H. pylori infection in healthy participants. Results: H. pylori infection is highly prevalent in the MENA region, mainly because of the accumulation of risk factors in developing countries. Herein, we highlight a lack of good quality studies on the prevalence of various H. pylori genotypes in the MENA region as well as a need for standard diagnostic methods and screening guidelines. Due to the complications associated with H. pylori, we recommend routine screening for H. pylori infection in all gastroenterology patients admitted in the MENA region. Conclusion: Concerted effort will first be required to validate affordable, non-invasive, and accurate diagnostic methods and to establish local guidelines with adapted cut-off values for the interpretation of the test results.

show abstract

“…Helicobacter pylori infects roughly half of the world’s human population and shares responsibility for gastric complications including stomach ulcers and gastric adenocarcinoma through its infection of gastric epithelial cells [ 172 , 173 , 174 ]. H. pylori utilizes a type IV protein secretion system (T4SS) involving the cytotoxin-associated gene L (CagL) adhesion tip protein to infect target cells with the virulence factor, cytotoxin-associated gene A (CagA) [ 175 , 176 ].…”

Section: Bacterial Use Of Non-ecm Integrin Ligandsmentioning

confidence: 99%

Beyond the Matrix: The Many Non-ECM Ligands for Integrins

LaFoya

Munroe

Miyamoto

et al. 2018

IJMS

View full text Add to dashboard Cite

The traditional view of integrins portrays these highly conserved cell surface receptors as mediators of cellular attachment to the extracellular matrix (ECM), and to a lesser degree, as coordinators of leukocyte adhesion to the endothelium. These canonical activities are indispensable; however, there is also a wide variety of integrin functions mediated by non-ECM ligands that transcend the traditional roles of integrins. Some of these unorthodox roles involve cell-cell interactions and are engaged to support immune functions such as leukocyte transmigration, recognition of opsonization factors, and stimulation of neutrophil extracellular traps. Other cell-cell interactions mediated by integrins include hematopoietic stem cell and tumor cell homing to target tissues. Integrins also serve as cell-surface receptors for various growth factors, hormones, and small molecules. Interestingly, integrins have also been exploited by a wide variety of organisms including viruses and bacteria to support infectious activities such as cellular adhesion and/or cellular internalization. Additionally, the disruption of integrin function through the use of soluble integrin ligands is a common strategy adopted by several parasites in order to inhibit blood clotting during hematophagy, or by venomous snakes to kill prey. In this review, we strive to go beyond the matrix and summarize non-ECM ligands that interact with integrins in order to highlight these non-traditional functions of integrins.

show abstract

Association of Helicobacter pylori cagA Gene with Gastric Cancer and Peptic Ulcer in Saudi Patients

Cited by 18 publications

References 47 publications

Performance of a Multiplex Serological Helicobacter pylori Assay on a Novel Microfluidic Assay Platform

Performance of a Multiplex Serological Helicobacter pylori Assay on a Novel Microfluidic Assay Platform

Prevalence of Helicobacter pylori Infection and Diagnostic Methods in the Middle East and North Africa Region

Beyond the Matrix: The Many Non-ECM Ligands for Integrins

Contact Info

Product

Resources

About