Association of gut dysbiosis with intestinal metabolites in response to antibiotic treatment

Khan, Tariq Jamal; Hasan, Mohammed Nihal; Azhar, Esam I.; Yasir, Muhammad

doi:10.1016/j.humic.2018.11.004

Cited by 16 publications

(14 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The median relative abundance of Bifidobacterium in our population was 1.76% (0-6.6) which is similar to the estimated abundance of Bifidobacterium of below 2% as described in US, South Korea, and Europe [41][42][43]. The Bifidobacterium genus is known to confer health benefits through metabolic activities such as involving in oligosaccharide fermentation [44], and a reduction in its relative abundance in human adult gut composition is an indicator of antibiotic use such as amoxicillin, ampicillin, and gentamicin, as described previously [45,46]. Interestingly, we observed a higher abundance of the Proteobacteria phylum (median 3.5% (1.3-9.0)) when compared to Europeans, Americans, and Asians in other areas (less than 2%) [43].…”

Section: Discussionsupporting

confidence: 87%

Cross-Sectional Analysis of the Microbiota of Human Gut and Its Direct Environment in a Household Cohort with High Background of Antibiotic Use

et al. 2021

View full text Add to dashboard Cite

Comprehensive insight into the microbiota of the gut of humans and animals, as well as their living environment, in communities with a high background of antibiotic use and antibiotic resistance genes is scarce. Here, we used 16S rRNA gene sequencing to describe the (dis)similarities in the microbiota of feces from humans (n = 107), domestic animals (n = 36), water (n = 89), and processed food (n = 74) in a cohort with individual history of antibiotic use in northern Vietnam. A significantly lower microbial diversity was observed among individuals who used antibiotics in the past 4 months (n = 44) compared to those who did not (n = 63). Fecal microbiota of humans was more diverse than nonhuman samples and shared a small part of its amplicon sequence variants (ASVs) with feces from animals (7.4% (3.2–9.9)), water (2.2% (1.2–2.8)), and food (3.1% (1.5–3.1)). Sharing of ASVs between humans and companion animals was not associated with the household. However, we did observe a correlation between an Enterobacteriaceae ASV and the presence of extended-spectrum beta-lactamase CTX-M-group-2 encoding genes in feces from humans and animals (p = 1.6 × 10−3 and p = 2.6 × 10−2, respectively), hinting toward an exchange of antimicrobial-resistant strains between reservoirs.

show abstract

Section: Discussionsupporting

confidence: 87%

Cross-Sectional Analysis of the Microbiota of Human Gut and Its Direct Environment in a Household Cohort with High Background of Antibiotic Use

et al. 2021

View full text Add to dashboard Cite

show abstract

“…2018; Khana et al . 2019). The current report revealed a high abundance of Verrucomicrobia phylum on the sixth day of vancomycin treatment.…”

Section: Resultsmentioning

confidence: 99%

“…The dysbiosis pattern of gut microbiota varied significantly with different types of antibiotics treatment (Khana et al . 2019). Treatment with neomycin caused an increase in the Bacteroidetes phylum while ampicillin caused a decrease in Firmicutes with significant elevation of Proteobacteria phylum (Rosa et al .…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of beneficial effects of vancomycin treatment on Th1‐ and Th2‐biased mice and the role of gut microbiota

2020

View full text Add to dashboard Cite

Aims The aim was to understand the time‐dependent antibiotic‐induced perturbation pattern of gut microbiota and its effect on the innate immune and metabolic profile of the host. Methods and Results Vancomycin was administered at 50 mg kg−1 of body weight twice daily for six consecutive days to perturb the gut microbiota of C57BL/6 (Th1‐biased) and BALB/c (Th2‐biased) mice. Following treatment with vancomycin, we observed a reduction in the abundance of phyla Firmicutes and Bacteroides and an increase in Proteobacteria in the gut for both strains of mice following treatment with vancomycin till day 4. Abundance of Akkermansia muciniphila of Verrucomicrobia phylum also increased, from day 5 onwards following vancomycin treatment. The time‐dependent variation of gut microbiota was associated with increased (i) expression of toll‐like receptors and inflammatory genes such as TNF‐α, IL‐6, and IL‐17, (ii) gut barrier permeability and (iii) blood glucose level of the host. The results also showed that (i) transplantation of cecal microbiota from vancomycin‐treated day 6 mice to day 3 vancomycin‐treated mice helped in restoring blood glucose level in C57BL/6 mice and (ii) short‐chain fatty acids like acetate, butyrate and propionate changed with the alteration of gut microbiota to induce differential regulation of host immune response. Conclusions The current results revealed that an increase in A. muciniphila led to decreased inflammation and increased rate of glucose tolerance in the host. The treatment, with vancomycin till day 4, increased expression of inflammatory genes. The continuation of vancomycin for two more days reversed the effects. The effects were significantly more in C57BL/6 than BALB/c mice. Significance and Impact of the Study The current study established that the treatment with vancomycin till day 4 increased pathogenic bacteria but day 5 onwards provided significant health‐related benefits to the host by increasing A. muciniphila more in C57BL/6 than BALB/c mice.

show abstract

“…The median relative abundance of Bifidobacterium in our population was 1.76% [0 – 6.6] which is similar to the estimated abundance of Bifidobacterium of below 2% as described in US, South Korea, and Europe (19, 20). The Bifidobacterium genus is known to confer health benefits through metabolic activities such as involving in oligosaccharide fermentation, but the increase of its relative abundance in human adult gut composition is an indicator of antibiotic use such as amoxicillin, ampicillin and gentamicin as described previously (21, 22). Interestingly, we observed a higher abundance of the Proteobacteria phylum (median 3.5% [1.3 – 9.0]) when compared to Europeans, Americans and Asians in other areas (less than 2%) (23).…”

Section: Discussionmentioning

confidence: 92%

Cross-sectional analysis of the microbiota of human gut and their direct environment (exposome) in a household cohort in northern Vietnam

Ngoc

Bich

Galazzo

et al. 2021

Preprint

View full text Add to dashboard Cite

Comprehensive insight into the human gut microbiota and the interaction with their environment in communities with a high background of antibiotic use and antibiotic resistance genes is currently largely lacking. In a cohort (Vietnam), individuals within the same household, also individuals within their geographical cluster share more bacterial taxa than individuals from different households or geographical clusters. The microbial diversity among individuals who used antibiotics in the past four months was significantly lower than those who did not. Fecal microbiota of humans was more diverse than non-human samples, shared a small part of its amplicon sequence variants (ASVs) with feces from animals (7.4%), water (2.2%) and food (3.1%). Sharing of ASVs between humans and companion animals was not associated with household. There is a correlation between an Enterobacteriaceae ASV and the presence of blactx-m-2 in feces from humans and animals, hinting towards an exchange of antimicrobial resistant strains between reservoirs.

show abstract

Association of gut dysbiosis with intestinal metabolites in response to antibiotic treatment

Cited by 16 publications

References 86 publications

Cross-Sectional Analysis of the Microbiota of Human Gut and Its Direct Environment in a Household Cohort with High Background of Antibiotic Use

Cross-Sectional Analysis of the Microbiota of Human Gut and Its Direct Environment in a Household Cohort with High Background of Antibiotic Use

Comparative analysis of beneficial effects of vancomycin treatment on Th1‐ and Th2‐biased mice and the role of gut microbiota

Cross-sectional analysis of the microbiota of human gut and their direct environment (exposome) in a household cohort in northern Vietnam

Contact Info

Product

Resources

About