Association of goat (Capra hircus) CD4 gene exon 6 polymorphisms with ability of sperm internalizing exogenous DNA

Wang, Lingbin; Fan, Jingsheng; Yu, Mingju; Zhao, Yongju

doi:10.1007/s11033-010-0272-2

Cited by 19 publications

(9 citation statements)

References 24 publications

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“…Polymorphisms of the CD4 gene have been reported in sheep (Boscariol et al, 2006) and goat (Wang et al, 2011). In swine, the polymorphisms of the CD4 gene have been reported only in miniature swine so far (Sundt et al, 1992; Gustafsson et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Association of the Porcine Cluster of Differentiation 4 Gene with T Lymphocyte Subpopulations and Its Expression in Immune Tissues

Xu¹,

Liu²,

et al. 2013

Asian Australas. J. Anim. Sci

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Cluster of differentiation 4 (CD4) is mainly expressed on CD4+ T cells, which plays an important role in immune response. The aim of this study was to detect the association between polymorphisms of the CD4 gene and T lymphocyte subpopulations in pigs, and to investigate the effects of genetic variation on the CD4 gene expression level in immune tissues. Five missense mutations in the CD4 gene were identified using DNA pooling sequencing assays, and two main haplotypes (CCTCC and AGCTG) in strong linkage disequilibrium (with frequencies of 50.26% and 46.34%, respectively) were detected in the population of Large White pigs. Our results indicated that the five SNPs and the two haplotypes were significantly associated with the proportions of CD4−CD8−, CD4+CD8+, CD4+CD8−, CD4+ and CD4+/CD8+ in peripheral blood (p<0.05). Gene expression analysis showed the mRNA level of the CD4 gene in thymus was significantly higher than that in lymph node and spleen (p<0.05). However, no significant difference was observed between animals with CCTCC/CCTCC genotype and animals with AGCTG/AGCTG genotype in the three immune tissues (p>0.05). These results indicate that the CD4 gene may influence T lymphocyte subpopulations and can be considered as a candidate gene affecting immunity in pigs.

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Section: Discussionmentioning

confidence: 99%

Association of the Porcine Cluster of Differentiation 4 Gene with T Lymphocyte Subpopulations and Its Expression in Immune Tissues

Xu¹,

Liu²,

et al. 2013

Asian Australas. J. Anim. Sci

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“…The CD4 receptor is polymorphic in a number of different species [9][10][11][12][13]. In humans, CD4 polymorphisms were correlated to some infectious diseases such as HIV infection [14] and chronic diseases such as type-1 diabetes [15].…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of monoclonal antibodies recognizing two CD4 isotypes of Microminipigs

et al. 2020

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Cluster of differentiation 4 (CD4) molecule expressed on the leukocytes is known to function as a co-receptor for class II major histocompatibility complex (MHC) binding to T cell receptor (TCR) on helper T cells. We previously identified two CD4 alleles (CD4.A and CD4.B) in a Microminipig population based on nucleotide sequencing and PCR detection of their gene sequences. However, CD4.B protein expression was not examined because of the unavailability of a reactive antibody to a CD4.B epitope. In this study, we have produced two swine-specific monoclonal antibodies (mAbs) against CD4.B molecules, one that recognizes only CD4.B (b1D7) and the other that recognizes both the CD4.A and CD4.B alleles (x1E10) and that can be used to distinguish CD4 T cell subsets by flow cytometry and immunohistochemistry. Using these two mAbs, we identified CD4.A and CD4.B allele-specific proteins on the surface of CD4.A (+/+) and CD4.B (+/+) T cells at a similar level of expression. Moreover, stimulation of peripheral blood mononuclear cells (PBMCs) derived from CD4.A (+/+) and CD4.B (+/+) swine with toxic shock syndrome toxin-1 (TSST-1) in vitro similarly activated both groups of cells that exhibited a slight increase in the CD4/CD8 double positive (DP) cell ratio. A large portion of the DP cells from the allelic CD4.A (+/+) and CD4.B (+/+) groups enhanced the total CD4 and class I swine leukocyte antigen (SLA) expression. The x1E10 mAb delayed and reduced the TSST-1-induced activation of CD4 T cells. Thus, CD4.B appears to be a functional protein whose expression on activated T cells is analogous to CD4.A.

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“…Progress in genetic and molecular techniques enabled the use of DNA polymorphism for the selection of superior animals, through Marker Assisted Selection (MAS). Recently many reports revealed the use of genetic variants in candidate genes as a marker for semen quality in swine (Huang et al, 2002;Wimmers et al, 2005;Lin et al, 2006) and goats (Wang et al, 2011). However, the reports on genetic control of fertility in bulls are scanty and need extensive investigation to meet the future needs.…”

Section: Introductionmentioning

confidence: 99%

Identification of Single Nucleotide Polymorphism in GnRHR gene in Murrah bulls

Reen

Ramesha

Preeti

et al. 2018

IJAR

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The present study was undertaken with the objectives of molecular characterization and detection of genetic polymorphism in the GnRHR gene in Murrah bulls. Blood samples were collected from 109 Murrah bulls maintained at three different organized semen stations viz., Centralized Semen Collection Centre of Livestock Breeding and Training Centre, Dharwad; Nandini Sperm Station and State Livestock Breeding and Training Centre, Hessarghatta, Bengaluru, Karnataka, India. Genomic DNA was isolated from the blood samples within 24 hours of collection . Mutations were screened using Polymerase Chain Reaction -Single Stranded Conformational Polymorphism (PCR-SSCP) technique followed by Sanger Sequencing. PCR-SSCP method revealed similar band pattern within Murrah bulls. To confirm monomorphism in the studied population with respect to GnRHR gene, duplicate samples from each primer fragment were custom sequenced. PCR-SSCP and sequence analysis revealed monomorphism within the studied population that is coding region as well as exon-intron boundaries of GnRHR gene is highly conserved among Murrah bulls. However, a total of 28 Single Nucleotide variations (SNV's) have been found when compared with Bos Taurus reference sequence for GnRHR gene.

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Association of goat (Capra hircus) CD4 gene exon 6 polymorphisms with ability of sperm internalizing exogenous DNA

Cited by 19 publications

References 24 publications

Association of the Porcine Cluster of Differentiation 4 Gene with T Lymphocyte Subpopulations and Its Expression in Immune Tissues

Association of the Porcine Cluster of Differentiation 4 Gene with T Lymphocyte Subpopulations and Its Expression in Immune Tissues

Preparation and characterization of monoclonal antibodies recognizing two CD4 isotypes of Microminipigs

Identification of Single Nucleotide Polymorphism in GnRHR gene in Murrah bulls

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