Association of Gene Polymorphisms in the Human MicroRNA-126 Gene with Plasma-Circulating MicroRNA-126 Levels and Acute Myocardial Infarction

Hu, Haiqiang; Yuan, Hong; Li, Cairong; Yu, Hong‐Ren; Chen, Yan

doi:10.1089/gtmb.2018.0282

Cited by 10 publications

(6 citation statements)

References 28 publications

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“…Therefore, we had reason to believe that an injection of EC-Exo would help motor fuction recover by delivering microRNA-126. The microRNA-126 gene single nucleotide polymorphisms (SNPs) rs4636297 and rs1140713 are associated with acute myocardial infarction (AMI), possibly by affecting the expression level of the microRNA-126 gene . It helped progenitor cell mobilization, increased the levels of circulating endothelial progenitor cells (EPC), and enhanced EPC function in vitro and in vivo, associated with SDF1/CXCR4 .…”

Section: Resultsmentioning

confidence: 99%

Brain Endothelial Cell-Derived Exosomes Induce Neuroplasticity in Rats with Ischemia/Reperfusion Injury

Gao

Zhou

Sun

et al. 2020

ACS Chem. Neurosci.

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Exosomes derived from the cerebral endothelial cells play essential roles in protecting neurons from hypoxia injury, but little is known regarding the biological effects and mechanisms of exosomes on brain plasticity. In this study, exosomes were isolated from rodent cerebral endothelial cells (bEnd.3 cells) by ultracentrifugation, either endothelial cell-derived exosomes (EC-Exo) or PBS was injected intraventricularly 2 h after the middle cerebral artery occlusion/reperfusion (MCAO/R) model surgery in the Exo group and control group, respectively. Sham group rats received the same surgical but not ischemic procedure. We evaluated the motor function of rats after MCAO/R, and the foot-fault rate of the Exo group was significantly lower than that of the control group within 23 days (p < 0.05); the Catwalk analysis also showed gait difference between two groups (p < 0.05). On day 28 after MCAO/R, we euthanized the rats, removed the motor cortex from the brain, and then sequenced the genes by using GO and KEGG to find transcriptome analysis of biological terms and functional annotations: The pathway enrichment revealed that the function of synaptic transmission, regulation of synaptic plasticity, and regulation of synaptic vesicle cycle was significantly enriched with the Exo group than control group. Furthermore, the upregulation of synapsin-I expression in the motor cortex (p < 0.05) as well as the increase of the length of the dendrites were found in the Exo group (p < 0.05) than the control group. We determined the content of exosome microRNA levels, and microRNA-126-3p was the highest (TPM) by transcriptome analysis. Moreover, the microRNA-126-3p protected PC12 cells from apoptosis and increased neurite outgrowth, illustrating the mechanism of how exosomes play a role in altering brain plasticity. This study demonstrated that EC-Exo promoted functional motor recovery in the MCAO/R model, exosomes were critical for the reconstruction of synaptic function in ischemic brain injury, and microRNA-126-3p from EC-Exo could serve as a treatment for nerve damage.

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Section: Resultsmentioning

confidence: 99%

Brain Endothelial Cell-Derived Exosomes Induce Neuroplasticity in Rats with Ischemia/Reperfusion Injury

Gao

Zhou

Sun

et al. 2020

ACS Chem. Neurosci.

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“…The rs4636297 (G/A) miRSNP is located in the flanking region of the miR‐126 gene within an intronic region of the epidermal growth factor‐like protein 7 ( EGFL7 ) gene, and it has been associated with post‐transcriptional alterations of circulating miR‐126 levels (https://www.ncbi.nlm.nih.gov/snp/rs4636297; accessed in April 27, 2020) (Harnprasopwat et al 2010; Hu et al 2019). The G allele inhibits the processing of the primary (pri) miR‐126 to the precursor (pre) miR‐126 , resulting in less mature miR‐126 in the cytoplasm.…”

Section: Discussionmentioning

confidence: 99%

Involvement of miR‐126 rs4636297 and miR‐146a rs2910164 polymorphisms in the susceptibility for diabetic retinopathy: a case–control study in a type 1 diabetes population

Massignam

Dieter

Pellenz

et al. 2020

Acta Ophthalmologica

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Background and purpose MicroRNAs (miRNAs) are small non‐coding RNAs that regulate gene expression. MiRNA‐126 and miRNA‐146a have been described as having abnormal expressions in diabetic retinopathy (DR) patients. Polymorphisms in genes codifying miRNAs (miRSNPs) may alter the expression of the corresponding miRNA and, thus, interfere with susceptibility to DR. Therefore, miRSNPs in miR‐126 and miR‐146a genes could be associated with DR susceptibility. The purpose of this study was to investigate the association between miR‐126 rs4636297 (G/A) and miR‐146a rs2910164 (G/C) miRSNPs and DR. Methods This case–control study included 195 type 1 diabetes mellitus (T1DM) patients with DR (cases) and 215 patients without DR and with ≥10 years of T1DM (controls). MiRSNPs were genotyped by real‐time PCR. Results Genotype distributions of two analysed miRSNPs were in Hardy–Weinberg equilibrium in controls (p > 0.050). Frequencies of the miR‐126 rs4636297 miRSNP were not significantly different between case and control groups (p = 0.169). However, after adjustment for age, glycated haemoglobin, triglycerides, estimated glomerular filtration rate and ethnicity, the A allele of this miRSNP was associated with protection for DR under additive [OR: 0.444 (95% CI: 0.211–0.936), p = 0.033] and dominant [OR: 0.512 (95% CI: 0.303–0.865), p = 0.012] inheritance models. Genotype and allele frequencies of miR‐146a rs2910164 miRSNP did not differ between groups (p = 0.368 and p = 0.957), and this polymorphism was not associated with DR when assuming different inheritance models. Conclusion Our results suggest an association between the A allele of miR‐126 rs4636297 miRSNP and protection for DR in a Southern Brazilian population.

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“…Various plasma miRNAs have been described as potential indicators of myocardial infarction, likely the most studied and described event among all cardiovascular diseases. Hence, miR-1, miR-126, miR-30a, miR-195, miR-26a-1, miR-146a, and miR-199a-1 were revealed to be upregulated in individuals diagnosed with acute MI (AMI) [65][66][67], with some of them, that is, miR-1, miR-126, miR-30a, and miR-195, reaching their maximal expression 8 h after the onset of symptoms [65,67,68]. In contrast, let-7b and miR-132-5p levels were decreased in acute MI patients when compared with control subjects [65].…”

Section: Myocardial Infarctionmentioning

confidence: 99%

Non-Coding RNAs as Blood-Based Biomarkers in Cardiovascular Disease

Videira

Martins

Falcão-Pires

2020

IJMS

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In 2020, cardiovascular diseases (CVDs) remain a leading cause of mortality and morbidity, contributing to the burden of the already overloaded health system. Late or incorrect diagnosis of patients with CVDs compromises treatment efficiency and patient’s outcome. Diagnosis of CVDs could be facilitated by detection of blood-based biomarkers that reliably reflect the current condition of the heart. In the last decade, non-coding RNAs (ncRNAs) present on human biofluids including serum, plasma, and blood have been reported as potential biomarkers for CVDs. This paper reviews recent studies that focus on the use of ncRNAs as biomarkers of CVDs.

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Association of Gene Polymorphisms in the Human MicroRNA-126 Gene with Plasma-Circulating MicroRNA-126 Levels and Acute Myocardial Infarction

Cited by 10 publications

References 28 publications

Brain Endothelial Cell-Derived Exosomes Induce Neuroplasticity in Rats with Ischemia/Reperfusion Injury

Brain Endothelial Cell-Derived Exosomes Induce Neuroplasticity in Rats with Ischemia/Reperfusion Injury

Involvement of miR‐126 rs4636297 and miR‐146a rs2910164 polymorphisms in the susceptibility for diabetic retinopathy: a case–control study in a type 1 diabetes population

Non-Coding RNAs as Blood-Based Biomarkers in Cardiovascular Disease

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