Association of Fusobacterium nucleatum infection and colorectal cancer: A Mexican study

Cuellar-Gomez, Hugo; Ocharan-Hernández, María Esther; Calzada‐Mendoza, Claudia C.; Comoto-Santacruz, David Alberto

doi:10.1016/j.rgmxen.2021.07.001

Cited by 9 publications

(7 citation statements)

References 39 publications

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“…Moreover, the RPA-CRISPR-Cas12a-Fn assay had lower requirements for personnel, environment, and equipment, allowing for rapid sample testing and identification under specific conditions. In contrast, qPCR requires a more stringent operating environment, complex detection equipment, and higher demands on laboratory personnel, resulting in a longer overall testing time ( 31 , 32 ). Therefore, the RPA-CRISPR-Cas12a-Fn assay has significant advantages over qPCR.…”

Section: Discussionmentioning

confidence: 99%

A rapid and sensitive CRISPR-Cas12a for the detection of Fusobacterium nucleatum

Qu,

Zhang,

et al. 2024

Microbiol Spectr

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Fusobacterium nucleatum (Fn), as a conditional pathogen, can cause a range of oral and gastrointestinal diseases. However, existing clinical detection methods require expensive equipment and complex procedures, which are inconvenient for large-scale screening in epidemiological research. The purpose of this study was to establish a reliable, rapid, and inexpensive detection method based on CRISPR/Cas12a technology for the detection of Fn. Specific recombinase polymerase amplification (RPA) primer sequences and crRNA sequences were designed based on the nusG gene of Fn. Subsequently, a fluorescence assay and a lateral flow immunoassay were established using the RPA and CRISPR-Cas12a system (RPA-CRISPR-Cas12a). Sensitivity validation revealed a limit of detection of 5 copies/µL. This method could distinguish Fn from other pathogens with excellent specificity. Furthermore, the RPA-CRISPR-Cas12a assay was highly consistent with the classical quantitative real-time PCR method when testing periodontal pocket samples. This makes it a promising method for the detection of Fn and has the potential to play an increasingly important role in infectious disease testing. IMPORTANCE Fusobacterium nucleatum (Fn) naturally exists in the microbial communities of the oral and gastrointestinal tracts of healthy individuals and can cause inflammatory diseases in the oral and gastrointestinal tracts. Recent studies have shown that Fn is closely associated with the occurrence and development of gastrointestinal cancer. Therefore, the detection of Fn is very important. Unlike the existing clinical detection methods, this study established a fluorescence-based assay and lateral flow immunoassay based on the RPA and CRISPR-Cas12a system (RPA-CRISPR-Cas12a), which is fast, reliable, and inexpensive and can complete the detection within 30–40 minutes. This makes it a promising method for the detection of Fn and has the potential to play an increasingly important role in infectious disease testing.

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Section: Discussionmentioning

confidence: 99%

A rapid and sensitive CRISPR-Cas12a for the detection of Fusobacterium nucleatum

Qu,

Zhang,

et al. 2024

Microbiol Spectr

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“…The used GAPDH forward primer is 5′-GTCTCCTCTGACTTCAACAGCG -3′ and reverse primer is 5′-ACCACCCTGTTGCTGTAGCCAA -3′. 17 Real time PCR was done using Applied Biosystems Ste-pOne Real-time PCR System. (Cat.…”

Section: Methodsmentioning

confidence: 99%

Study of Long Non-Coding RNA Tug1 Expression in Egyptian Colorectal Adenocarcinoma Patients

Amin,

Ketat,

Kamal

et al. 2023

Journal of Coloproctology

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Purpose Colorectal cancer (CRC) is one of the most fatal tumors worldwide. In Egypt, most CRC cases occur in individuals > 40 years old. TUG1 has been proved to be disrupted in different malignancies and may have a critical role in tumor progression, invasion, and metastasis. However, its role in CRC has not been adequately studied. Materials / Methods Quantitative real-time polymerase chain reaction (PCR) was used to evaluate the expression levels of long non-coding RNA (LncRNA) taurine upregulated gene 1 (TUG1), in nonmetastatic and metastatic CRC tissues and adjacent noncancerous tissues as control. Results LncRNA TUG1 expression was significantly upregulated in both nonmetastatic and metastatic CRC tissues, in comparison with the adjacent noncancerous tissue. It was found that TUG1 could have a possible prognostic role in CRC, by comparing the sensitivity and specificity of TUG1 with those of CEA and CA19–9. Conclusion The results of the current study suggest that the LncRNA TUG1 participates in the malignant behaviors of CRC cells.

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“…Fusobacterium nucleatum is highly enriched in tumor tissue samples from patients diagnosed with CRC. CRC is associated with the disruption of the colonic architecture with increased immune cell infiltration and depleted mucus layers [ 73 , 74 ]. Lactic acid bacteria (LAB) strains potently activate intracellular sensors, including stimulator of IFN genes (STING) and mitochondrial antiviral signaling (MAVS) that result in type I IFN (IFN-I) production, cytoprotective responses, and prevention of overreactive NF-κB-dependent inflammation in the gut [ 75 ].…”

Section: Cancer—microbiome—immune Axismentioning

confidence: 99%

The Effect of the Gut Microbiota on Systemic and Anti-Tumor Immunity and Response to Systemic Therapy against Cancer

Aghamajidi

Vareki

2022

Cancers

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Gut microbiota can have opposing functions from pro-tumorigenic to anti-tumorigenic effects. Increasing preclinical and clinical evidence suggests that the intestinal microbiota affects cancer patients’ response to immune checkpoint inhibitors (ICIs) immunotherapy, such as anti-programmed cell death protein 1 (PD-1) and its ligand (PD-L1) and anti-cytotoxic T lymphocyte-associated protein 4 (CTLA-4). Microbiota-induced inflammation possibly contributes to tumor growth and cancer development. Microbiota-derived metabolites can also be converted to carcinogenic agents related to genetic mutations and DNA damage in organs such as the colon. However, other attributes of microbiota, such as greater diversity and specific bacterial species and their metabolites, are linked to better clinical outcomes and potentially improved anti-tumor immunity. In addition, the intratumoral microbial composition strongly affects T-cell-mediated cytotoxicity and anti-tumor immune surveillance, adding more complexity to the cancer-microbiome-immune axis. Despite the emerging clinical evidence for the activity of the gut microbiota in immuno-oncology, the fundamental mechanisms of such activity are not well understood. This review provides an overview of underlying mechanisms by which the gut microbiota and its metabolites enhance or suppress anti-tumor immune responses. Understanding such mechanisms allows for better design of microbiome-specific treatment strategies to improve the clinical outcome in cancer patients undergoing systemic therapy.

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Association of Fusobacterium nucleatum infection and colorectal cancer: A Mexican study

Cited by 9 publications

References 39 publications

A rapid and sensitive CRISPR-Cas12a for the detection of Fusobacterium nucleatum

A rapid and sensitive CRISPR-Cas12a for the detection of Fusobacterium nucleatum

Study of Long Non-Coding RNA Tug1 Expression in Egyptian Colorectal Adenocarcinoma Patients

The Effect of the Gut Microbiota on Systemic and Anti-Tumor Immunity and Response to Systemic Therapy against Cancer

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