Association of four new single-nucleotide polymorphisms in follicle-stimulating hormone receptor and zona pellucida glycoprotein with reproductive traits in pigs

Abstract

Two new single-nucleotide polymorphisms (SNPs) (C1166T and G1190A) were discovered in the follicle-stimulating hormone receptor (FSHR) gene and two (G261A and T302C) in the zona pellucida glycoprotein (ZP3) gene. These SNPs were genotyped in three Chinese domestic purebred sow lines (42 Small Meishan, 46 Qingping and 41 Jinhua sows) and three European purebred sow lines (225 Duroc, 195 Large White and 65 Landrace sows) by using SNP chips. Phenotypic data including the functional teat number (i.e. milk-producing teats, TN) and number of piglets born alive per litter (NBA). These traits were tested for association with the genotypes of four SNPs. The association analysis revealed genotype of G261A in the ZP3 gene was significantly (P , 0.01) associated with overall NBA and NBA at later parities (NBA 21 ) but not with NBA at first parity (NBA 1 ). There was a significant (P , 0.05) difference between sows with genotype GG (14.83 6 0.18) and AA (14.26 6 0.09) in TN at position 261 in the ZP3 gene. No significant associations were observed for the SNPs in the FSHR gene with NBA or TN in our populations. The results showed that the new SNPs in the ZP3 gene may be an effective potential marker to be used in conjunction with traditional selection methods.

Association analysis between pseudorabies antibody and five single-nucleotide polymorphisms in pigs

“…where Y is the observed antibody, X the effects of genotype, W the effects of farms, e the effects of random; the data analysis was performed according to the protocol (Yuan et al, 2007a and2007b). Figure 2 The image of microarray genotyping and the three genotype groups of single-nucleotide polymorphisms from EHMT2 gene.…”