Association of florfenicol residues with the abundance of oxazolidinone resistance genes in livestock manures

Wang, Yingyu; Li, Xiaowei; Fu, Yulin; Chen, Yiqiang; Wang, Yang; Ye, Dongyang; Wang, Chengfei; Han, Xue; Zhou, Lanzhang; Du, Jingjing; Shen, Jianzhong; Xia, Xi

doi:10.1016/j.jhazmat.2020.123059

Cited by 50 publications

(25 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The high mcr-1 positive detection rate in the rumen of dairy cows (81%) and dairy buffaloes (100%) indicates the mcr-1 widespread in dairy cows and buffaloes in the south of China, thus the use of colistin in dairy industry should be reconsidered. Linezolid is one of the last antimicrobial treatment options in human clinical medicine and has not been approved for applying in livestock industry [ 6 ]. However, several linezolid resistant genes ( cfrA , cipA , clbA , clbB , clbC ) with high relative abundance (average CPM > 600 for each gene) were identified in the rumen samples of dairy cows and dairy buffaloes, and they also confirmed in other studies [ 59 , 60 ].…”

Section: Discussionmentioning

confidence: 99%

“…The wide spread of antimicrobial resistance genes (ARGs), especially for those that confer resistance to clinical relevance or “last defense line” antibiotics, requires urgent tasks to reveal the harbor sources and transmission routes of diverse ARGs [ 2 ]. The microbiome of livestock gastrointestinal tracts serves as important reservoirs for ARGs [ 3 – 6 ], which is largely attributed to the long-term use of antibiotic in veterinary medicine or sub-therapeutic doses of antibiotic supplied in the feed acting as growth promoter [ 7 ]. The microbial ARGs are mainly structured by the bacterial phylogeny [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Metagenomics analysis revealed the distinctive ruminal microbiome and resistive profiles in dairy buffaloes

et al. 2021

View full text Add to dashboard Cite

Background Antimicrobial resistance poses super challenges in both human health and livestock production. Rumen microbiota is a large reservoir of antibiotic resistance genes (ARGs), which show significant varations in different host species and lifestyles. To compare the microbiome and resistome between dairy cows and dairy buffaloes, the microbial composition, functions and harbored ARGs of rumen microbiota were explored between 16 dairy cows (3.93 ± 1.34 years old) and 15 dairy buffaloes (4.80 ± 3.49 years old) using metagenomics. Results Dairy buffaloes showed significantly different bacterial species (LDA > 3.5 & P < 0.01), enriched KEGG pathways and CAZymes encoded genes (FDR < 0.01 & Fold Change > 2) in the rumen compared with dairy cows. Distinct resistive profiles were identified between dairy cows and dairy buffaloes. Among the total 505 ARGs discovered in the resistome of dairy cows and dairy buffaloes, 18 ARGs conferring resistance to 16 antibiotic classes were uniquely detected in dairy buffaloes. Gene tcmA (resistance to tetracenomycin C) presented high prevalence and age effect in dairy buffaloes, and was also highly positively correlated with 93 co-expressed ARGs in the rumen (R = 0.98 & P = 5E-11). In addition, 44 bacterial species under Lactobacillus genus were found to be associated with tcmA (R > 0.95 & P < 0.001). L. amylovorus and L. acidophilus showed greatest potential of harboring tcmA based on co-occurrence analysis and tcmA-containing contigs taxonomic alignment. Conclusions The current study revealed distinctive microbiome and unique ARGs in dairy buffaloes compared to dairy cattle. Our results provide novel understanding on the microbiome and resistome of dairy buffaloes, the unique ARGs and associated bacteria will help develop strategies to prevent the transmission of ARGs.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metagenomics analysis revealed the distinctive ruminal microbiome and resistive profiles in dairy buffaloes

et al. 2021

View full text Add to dashboard Cite

show abstract

“…S. epidermis is most commonly isolated from humans [24], while in this work, it was present in the animals as well as seafood. with ATP-binding cassette transporter gene optrA and it has been identified in bacteria of the animal origin [28,29]. However, in this work, the optrA gene has not been identified in these strains.…”

Section: Bacterial Strains and Antimicrobial Susceptibility Testingmentioning

confidence: 71%

Characterization of florfenicol resistance genes in the coagulase-negative Staphylococcus (CoNS) isolates and genomic features of a multidrug-resistant Staphylococcus lentus strain H29

Zhang

Liang

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundWith the wide use of florfenicol to prevent and treat the bacterial infection of domestic animals, the emergence of the florfenicol resistance bacteria is increasingly serious. It is very important to elucidate the molecular mechanism of the bacteria’s resistance to florfenicol. MethodsThe minimum inhibitory concentration (MIC) levels was determined by the agar dilution method, and polymerase chain reaction (PCR) was conducted to analyze the distribution of florfenicol resistance genes in 39 CoNS strains isolated from poultry and livestock animals and seafood. The whole genome sequence of one multidrug-resistant strain, Staphylococcus lentus H29, was characterized, and comparative genomics analysis of the resistance gene-related sequences was also performed. ResultsAs a result, the isolates from the animals showed a higher resistance rate (23/28, 82.1%) and much higher MIC levels of florfenicol than those from seafood. Twenty-seven animal isolates carried 37 florfenicol resistance genes (including 26 fexA , 6 cfr and 5 fexB genes), of which 1 carried a cfr gene, 16 carried a fexA gene, 5 carried both fexA and fexB genes and 5 carried both fexA and cfr genes. On the other hand, all 11 isolates from seafood were sensitive to florfenicol, and only 3 carried a fexA gene each. The whole genome sequence of S. lentus H29 was composed of a chromosome and two plasmids ( pH29-46 , pH29- 26) and harbored 11 resistance genes, including 6 genes [ cfr, fexA, ant(6)-Ia , aac A -aph D , mecA and mph(C) ] encoded on the chromosome, four genes [ cfr, fexA, aac A -aph D and tcaA ] on pH29-46 and one gene ( fosD ) on pH29-26. It was interested to find that the S. lentus H29 genome carried two identical copies of the gene arrays of radC - tnpABC - hp - fexA (5,671 bp) and IS 256 - cfr (2,690 bp), of which one copy of the two gene arrays was encoded on plasmid pH29-46, while the other was encoded on the chromosome. ConclusionsThe current study revealed the wide distribution of florfenicol resistance genes ( cfr, fexA and fexB ) in animal bacteria, and to the best of our knowledge, this is the first report of one CoNS strain carrying two identical copies of florfenicol resistance-related gene arrays.

show abstract

“…In China, florfenicol has been widely used in food-producing animals [ 21 ]. It was reported that the emergence of oxazolidinone resistance genes such as poxtA is closely related to the use of florfenicol in breeding farms [ 22 ]. Antimicrobial susceptibility testing showed that all donors and electrotransformants were resistant to florfenicol, indicating poxtA -positive plasmids could be directly selected by florfenicol.…”

Section: Discussionmentioning

confidence: 99%

Identification of the Multiresistance Gene poxtA in Oxazolidinone-Susceptible Staphylococcus haemolyticus and Staphylococcus saprophyticus of Pig and Feed Origins

Chen

Liu

et al. 2021

Pathogens

View full text Add to dashboard Cite

Previous studies on the prevalence and transmission mechanism of oxazolidinone resistance gene poxtA in CoNS are lacking, which this study addresses. By screening 763 CoNS isolates from different sources of several livestock farms in Guangdong, China, 2018–2020, we identified that the poxtA was present in seven CoNS isolates of pig and feed origins. Species identification and multilocus sequence typing (MLST) confirmed that seven poxtA-positive CoNS isolates were composed of five ST64-Staphylococcus haemolyticus and two Staphylococcus saprophyticus isolates. All poxtA-positive Staphylococcus haemolyticus isolates shared similar pulsed-field gel electrophoresis (PFGE) patterns. Transformation assays demonstrated all poxtA-positive isolates were able to transfer poxtA gene to Staphylococcus aureus RN4220. S1-PFGE and whole-genome sequencing (WGS) revealed the presence of poxtA-carrying plasmids in size around 54.7 kb. The plasmid pY80 was 55,758 bp in size and harbored the heavy metal resistance gene czcD and antimicrobial resistance genes, poxtA, aadD, fexB and tet(L). The regions (IS1216E-poxtA-IS1216E) in plasmid pY80 were identified in Staphylococcus spp. and Enterococcus spp. with different genetic and source backgrounds. In conclusion, this was the first report about the poxtA gene in Staphylococcus haemolyticus and Staphylococcus saprophyticus, and IS1216 may play an important role in the dissemination of poxtA among different Gram-positive bacteria.

show abstract

Association of florfenicol residues with the abundance of oxazolidinone resistance genes in livestock manures

Cited by 50 publications

References 25 publications

Metagenomics analysis revealed the distinctive ruminal microbiome and resistive profiles in dairy buffaloes

Metagenomics analysis revealed the distinctive ruminal microbiome and resistive profiles in dairy buffaloes

Characterization of florfenicol resistance genes in the coagulase-negative Staphylococcus (CoNS) isolates and genomic features of a multidrug-resistant Staphylococcus lentus strain H29

Identification of the Multiresistance Gene poxtA in Oxazolidinone-Susceptible Staphylococcus haemolyticus and Staphylococcus saprophyticus of Pig and Feed Origins

Contact Info

Product

Resources

About