Association of fecal and serum <scp>microRNA</scp> profiles with gastrointestinal cancer and chronic inflammatory enteropathy in dogs

Lyngby, Janne G; Gòdia, Marta; Brogaard, Louise; Kristensen, Annemarie T.; Fredholm, Merete; Skancke, Ellen; Morris, Joanna S.; Dupont, Nana Hee; Schmitz, Silke Salavati; Argyle, David; Sánchez, Armand; Bjørnvad, Charlotte Reinhard; Cirera, Susanna; Nielsen, Lise Nikolic

doi:10.1111/jvim.16530

Cited by 6 publications

(18 citation statements)

References 112 publications

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“…In our study, serum miR‐223‐3p could distinguish between cats with GIC and CIE with an AUC of 0.90 (95% CI, 0.760‐1.0). A previous study reported similar performance with an AUC of 0.92 for this miRNA in feces for differentiating between GIC and CIE dogs 12 . MiR‐223‐3p was included in our study because it was previously found to be a potential fecal biomarker candidate for differentiation between GIC and CIE in dogs 12 .…”

Section: Discussionmentioning

confidence: 77%

“…All healthy cats were recruited at UCPH. A study investigating miRNA in feces and serum from dogs using the same methodology has previously been published by this group 12 …”

Section: Methodsmentioning

confidence: 99%

“…The qPCR was carried out using the high‐throughput platform Biomark HD (Fluidigm, San Francisco, California) and the 96.96 Dynamic Array integrated fluidic circuit chip format (Fluidigm, San Francisco, California). All details on RT‐qPCR analysis can be found in File S1 and in Table S1 12‐14,19‐47 …”

Section: Methodsmentioning

confidence: 99%

“…In humans, miRNAs have shown great potential as biomarkers because of their stability and accessibility in serum and feces, and their ubiquitous involvement in cancer development and progression 10,11 . Likewise, several miRNAs are abundant in GIC and chronic enteropathies in dogs 12,13 . MiRNAs already have been successfully measured in feces from healthy cats, 14 but their potential as non‐invasive diagnostic biomarkers in serum and feces for differentiation between GIC and CIE in cats remains to be investigated.…”

Section: Introductionmentioning

confidence: 99%

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Association of serum and fecal microRNA profiles in cats with gastrointestinal cancer and chronic inflammatory enteropathy

Brogaard,

Lyngby,

Kristensen

et al. 2023

Veterinary Internal Medicne

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BackgroundDifferentiation of gastrointestinal cancer (GIC) from chronic inflammatory enteropathies (CIE) in cats can be challenging and often requires extensive diagnostic testing. MicroRNAs (miRNAs) have promise as non‐invasive biomarkers in serum and feces for diagnosis of GIC.Hypothesis/ObjectivesCats with GIC will have serum and fecal miRNA profiles that differ significantly from healthy cats and cats with CIE. Identify serum and fecal miRNAs with diagnostic potential for differentiation between cats with GIC and CIE as compared to healthy cats.AnimalsTen healthy cats, 9 cats with CIE, and 10 cats with GIC; all client‐owned.MethodsCats were recruited for an international multicenter observational prospective case‐control study. Serum and feces were screened using small RNA sequencing for miRNAs that differed in abundance between cats with GIC and CIE, and healthy cats. Diagnostic biomarker potential of relevant miRNAs from small RNA sequencing and the literature was confirmed using reverse transcription quantitative real‐time PCR (RT‐qPCR).ResultsSerum miR‐223‐3p was found to distinguish between cats with GIC and CIE with an area under the curve (AUC) of 0.9 (95% confidence interval [CI], 0.760‐1.0), sensitivity of 90% (95% CI, 59.6‐99.5%), and specificity of 77.8% (95% CI, 45.3‐96.1%). Serum miR‐223‐3p likewise showed promise in differentiating a subgroup of cats with small cell lymphoma (SCL) from those with CIE. No fecal miRNAs could distinguish between cats with GIC and CIE.Conclusion and Clinical ImportanceSerum miR‐223‐3p potentially may serve as a noninvasive diagnostic biomarker of GIC in cats, in addition to providing a much needed tool for the differentiation of CIE and SCL.

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Section: Discussionmentioning

confidence: 77%

“…All healthy cats were recruited at UCPH. A study investigating miRNA in feces and serum from dogs using the same methodology has previously been published by this group 12 …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Association of serum and fecal microRNA profiles in cats with gastrointestinal cancer and chronic inflammatory enteropathy

Brogaard,

Lyngby,

Kristensen

et al. 2023

Veterinary Internal Medicne

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“…Circulating miR-20b was described as a potential biomarker for distinguishing gastrointestinal cancer from chronic inflammatory enteropathy in dogs, which probably reflects the important role of miR-20b in intestinal diseases [ 18 ]. Decrease in miR-192 expression (together with the shift in other miRNAs) was described in the mucosa and serum of dogs with large intestinal inflammatory bowel disease [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

miRNA Profiles of Canine Intestinal Carcinomas, Lymphomas and Enteritis Analysed by Digital Droplet PCR from FFPE Material

Kehl

Valkai

Weyer

et al. 2023

Veterinary Sciences

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Most canine intestinal tumours are B-cell or T-cell lymphomas or carcinomas. They have to be distinguished from cases of enteritis. Non-invasive biomarkers such as miRNAs would be a step towards faster diagnosis. The aim of this study was to investigate shifts in miRNA expression in tissue samples collected from cases of enteritis, carcinoma and lymphoma of the small and large intestine to better understand the potential of miRNA as biomarkers for tumour diagnosis and classification. We selected two oncogenic miRNAs (miR-18b and 20b), two tumour suppressive miRNAs (miR-192 and 194) and two potential biomarkers for neoplasms (miR-126 and 214). They were isolated from FFPE material, quantified by ddPCR, normalised with RNU6B and compared with normal tissue values. Our results confirmed that ddPCR is a suitable method for quantifying miRNA from FFPE material. Expression of miR-18b and miR-192 was higher in carcinomas of the small intestine than in those of the large intestine. Specific miRNA patterns were observed in cases of enteritis, B-cell and T-cell lymphoma and carcinoma. However, oncogenic miR-18b and 20b were not elevated in any group and miR-126 and 214 were down-regulated in T-cell and B-cell lymphoma, as well as in carcinomas and lymphoplasmacytic enteritis of the small intestine.

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Characterization of canine intestinal microRNA expression in inflammatory bowel disease and T-cell lymphoma

Irving

Hiron

Davison

et al. 2023

Journal of Comparative Pathology

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Association of fecal and serum microRNA profiles with gastrointestinal cancer and chronic inflammatory enteropathy in dogs

Cited by 6 publications

References 112 publications

Association of serum and fecal microRNA profiles in cats with gastrointestinal cancer and chronic inflammatory enteropathy

Association of serum and fecal microRNA profiles in cats with gastrointestinal cancer and chronic inflammatory enteropathy

miRNA Profiles of Canine Intestinal Carcinomas, Lymphomas and Enteritis Analysed by Digital Droplet PCR from FFPE Material

Characterization of canine intestinal microRNA expression in inflammatory bowel disease and T-cell lymphoma

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