Association of FcγRIIB and FcγRIIA R131H gene polymorphisms with renal involvement in Egyptian systemic lupus erythematosus patients

Abstract

Identification of the genetic basis of systemic lupus erythematosus (SLE) may contribute to the discovery of effective drugs before renal involvement. Our aim of this study was to estimate the association between Fc gamma receptor (FcγR) polymorphisms and SLE and renal involvement in Egyptian patients. FcγRIIB and FcγRIIA R131H gene polymorphisms were genotyped in 180 Egyptian adults. Genotyping for FcγRIIA R131H was performed using allele-specific PCR and FcγRIIB-Ile232 Thr polymorphism was genotyped using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The study showed that the homozygous genotype (Thr/Thr) of FcγRIIB significantly increased in all SLE patients (90 patients) and in SLE patients complicated with nephritis (61 patients). The Thr allele was significantly associated with an increased risk of the disease in all the patients and in patients complicated with nephritis. Our study demonstrated an association of FcγRIIB polymorphisms with SLE and lupus nephritis and a lack of association of FcγRIIA polymorphisms with SLE in the Egyptian patients.

Auto-antigenic Properties of the Spliceosome as a Molecular Tool for Diagnosing Systemic Lupus Erythematosus and Mixed Connective Tissue Disease Patients

“…In congruency with previous reports (Vlachoyiannopoulos et al, 1996;Luyckx et al, 2005;Somarelli et al, 2011;Watanabe et al, 2012;Gunnarsson et al, 2013;Zidan et al, 2013), the analysis of 187 medical records documented for SLE and MCTD patients revealed that 35 clinical manifestations and molecular features are significantly different in the two disease states (Figure 8). The prevalence of skin rashes in SLE while myositis and muscle weakness was presented in MCTD was expected since these are typical clinical manifestation associated with each of these autoimmune illnesses (Uthman et al, 1996;Belibou et al, 2012;Szodoray et al, 2012).…”

“…Given that these results and others have identified specific organ involvement in either SLE or MCTD patients (Figure 8) (Vlachoyiannopoulos et al, 1996;Luyckx et al, 2005;Watanabe et al, 2012;Gunnarsson et al, 2013;Zidan et al, 2013), it highlights the clinical value of MCTD diagnosis given that the type of medical regime delivered to a patient will rely on the potential development of specific organ(s) malfunction. Aware of the lack of methods designed to segregate between these autoimmune disorders (Table 6 and Figure 11), we described eight different novel classification rules (Tables 3 and 4 represents the best classifier from those evaluated with a power of 93% to distinguish between SLE and MCTD patients (Figure 12).…”

“…The prevalence of skin rashes in SLE while myositis and muscle weakness was presented in MCTD was expected since these are typical clinical manifestation associated with each of these autoimmune illnesses (Uthman et al, 1996;Belibou et al, 2012;Szodoray et al, 2012). Like others (Watanabe et al, 2012;Gunnarsson et al, 2013;Zidan et al, 2013), the data showed that specific organ malfunction was involved with each of these syndromes where renal disease was elevated in SLE cohort while myocardial infarction was more frequently in the MCTD population (p ≤ 0.002). Additionally, the correlation analyses support the idea that SLE and MCTD are indeed separate disorders given that unique correlations of molecular markers and clinical symptoms are observed in either SLE or MCTD cohorts (Figures 2 and 3).…”

“…Moreover, even though some SLE patients develop anti-U1 snRNP response; they are able to retain IgM reactivity against these antigens while those with MCTD class switch to IgG response instead (Vlachoyiannopoulos et al, 1996;Somarelli et al, 2011;. Likewise, severe renal and central nervous system (CNS) manifestations are observed in SLE patients (Zidan et al, 2013) while lung and heart malfunctions are frequent in MCTD subjects (Watanabe et al, 2012;Gunnarsson et al, 2013).…”

Immunogenetic studies of Guillain - Barré syndrome and chronic inflammatory demyelinating polyradiculoneuropathy

“…Polymorphisms in these receptors have been associated with infectious diseases and autoimmunity (Zidan, Sabbah et al 2013). Genotyping of 80 GBS patients and 80 healthy controls from North India found that polymorphisms in genes encoding FcγRIIA and FCγRIIIA, but not FcγRIIIB, were associated with GBS (Geleijns, Emonts et al 2007).…”