Association of Expression Aberrances and Genetic Polymorphisms of <i>Lysyl Oxidase</i> with Areca-Associated Oral Tumorigenesis

Shieh, Tzong‐Ming; Lin, Shu Chun; Liu, Chung Ji; Chang, Shu Shin; Ku, Ti Hsuan; Chang, Kuo Wei

doi:10.1158/1078-0432.ccr-06-2685

Cited by 64 publications

(78 citation statements)

References 18 publications

(44 reference statements)

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“…Cell culture, reagents, and phenotypic assays. The HNSCC cell lines Fadu, OECM-1, and SAS cells; HT29 CRC cells; and 293FT and phoenix package cells were cultured as previously described (2,29), as were normal human oral keratinocytes (NHOK), which were used as controls (30). Fadu and SAS were tumorigenic, whereas OECM-1 was nontumorigenic in nude mice (31).…”

Section: Methodsmentioning

confidence: 99%

“…VEGF production in aliquots of cell supernatant or tissue lysate was analyzed using a kit (Invitrogen). Analysis of cell viability, proliferation, migration, and anchorage-independent colony formation followed the protocols previously published (2).…”

Section: Methodsmentioning

confidence: 99%

“…Cell lysate (80 μg), cytosolic fraction (80 μg), and nuclear extract (40 μg) were isolated using previously described protocols (27). Western blot analysis followed previously used protocols (2). The providers and dilution of the primary antibodies are presented in Supplementary Table S2.…”

Section: Methodsmentioning

confidence: 99%

“…Head and neck squamous cell carcinoma (HNSCC) is the fifth most common carcinoma worldwide (1,2). MicroRNAs (miRNA) are 19 to 24 nucleotides noncoding RNAs that regulate the translation and degradation of target mRNAs (3,4).…”

Section: Introductionmentioning

confidence: 99%

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miR-31 Ablates Expression of the HIF Regulatory Factor FIH to Activate the HIF Pathway in Head and Neck Carcinoma

et al. 2010

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MicroRNAs (miRNA) are endogenously expressed noncoding RNAs with important biological and pathological functions that are yet to be fully defined. This study investigated alterations in miRNA expression in head and neck squamous cell carcinoma (HNSCC), the incidence of which is rising throughout the world. Initial screening and subsequent analysis identified a panel of aberrantly expressed miRNAs in HNSCC tissues, with miR-31 among the most markedly upregulated. Ectopic expression of miR-31 increased the oncogenic potential of HNSCC cells under normoxic conditions in cell culture or tumor xenografts. Conversely, blocking miR-31 expression reduced the growth of tumor xenografts. The in silico analysis suggested that miR-31 may target the 3′ untranslated region (UTR) of factor-inhibiting hypoxia-inducible factor (FIH), a hypoxia-inducible factor (HIF) regulatory factor that inhibits the ability of HIF to act as a transcriptional regulator under normoxic conditions. In support of this likelihood, miR-31 expression repressed FIH expression and mutations within the predictive miR-31 target site in the FIH 3′ UTR abrogated FIH repression. Furthermore, miR-31 expression increased HIF transactivation activity. We found that FIH suppressed oncogenic phenotypes under normoxic conditions and that this activity was abrogated by functional mutations. Lastly, increased miR-31 expression was correlated with decreased levels of FIH in tumor tissues. Our findings suggest that miR-31 contributes to the development of HNSCC by impeding FIH to activate HIF under normoxic conditions. Cancer Res; 70(4); 1635-44. ©2010 AACR.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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miR-31 Ablates Expression of the HIF Regulatory Factor FIH to Activate the HIF Pathway in Head and Neck Carcinoma

et al. 2010

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“…siRNA for mouse LOX was 5 0 -CAACGGGCAGGUGUUCAG-3 0 and 5 0 -CUGAACACCUGCCCGUUG-3 0 (ref. 54). As a control, siRNA duplex with irrelevant sequence (QIAGEN) was used.…”

Section: Methodsmentioning

confidence: 99%

Control of lung vascular permeability and endotoxin-induced pulmonary oedema by changes in extracellular matrix mechanics

et al. 2013

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Increased vascular permeability contributes to many diseases, including acute respiratory distress syndrome, cancer and inflammation. Most past work on vascular barrier function has focused on soluble regulators, such as tumour-necrosis factor-a. Here we show that lung vascular permeability is controlled mechanically by changes in extracellular matrix structure. Our studies reveal that pulmonary vascular leakage can be increased by altering extracellular matrix compliance in vitro and by manipulating lysyl oxidase-mediated collagen crosslinking in vivo. Either decreasing or increasing extracellular matrix stiffness relative to normal levels disrupts junctional integrity and increases vascular leakage. Importantly, endotoxin-induced increases of vascular permeability are accompanied by concomitant increases in extracellular matrix rigidity and lysyl oxidase activity, which can be prevented by inhibiting lysyl oxidase activity. The identification of lysyl oxidase and the extracellular matrix as critical regulators of lung vascular leakage might lead to the development of new therapeutic approaches for the treatment of pulmonary oedema and other diseases caused by abnormal vascular permeability.

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Association between areca‐stimulated vimentin expression and the progression of head and neck cancers

Tseng¹,

Chang²,

Yang³

et al. 2011

Head & Neck

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Association of Expression Aberrances and Genetic Polymorphisms of Lysyl Oxidase with Areca-Associated Oral Tumorigenesis

Cited by 64 publications

References 18 publications

miR-31 Ablates Expression of the HIF Regulatory Factor FIH to Activate the HIF Pathway in Head and Neck Carcinoma

miR-31 Ablates Expression of the HIF Regulatory Factor FIH to Activate the HIF Pathway in Head and Neck Carcinoma

Control of lung vascular permeability and endotoxin-induced pulmonary oedema by changes in extracellular matrix mechanics

Association between areca‐stimulated vimentin expression and the progression of head and neck cancers

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