Association of Exosomal miR-210 with Signaling Pathways Implicated in Lung Cancer

Chen, Qiao Yi; Xie, Xiaoge

doi:10.3390/genes12081248

Cited by 12 publications

(8 citation statements)

References 149 publications

(160 reference statements)

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“…The interaction of miR-210 with the BNIP3 transcript (significantly upregulated in PANF) can protect cancer cells against hypoxic damage [ 49 ]. miR-210 also regulates signalling cascades with the central position of the TIMP1 gene [ 50 ] non-significantly upregulated in PANF. The TIMP1 protein is known as an inhibitor of metalloproteinases participating in the remodelling of the extracellular matrix in tumors [ 51 ].…”

Section: Resultsmentioning

confidence: 99%

Expression of Selected miRNAs in Normal and Cancer-Associated Fibroblasts and in BxPc3 and MIA PaCa-2 Cell Lines of Pancreatic Ductal Adenocarcinoma

Mandys

Popov

Gürlich

et al. 2023

IJMS

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Therapy for pancreatic ductal adenocarcinoma remains challenging, and the chances of a complete cure are very limited. As in other types of cancer, the expression and role of miRNAs in controlling the biological properties of this type of tumor have been extensively studied. A better insight into miRNA biology seems critical to refining diagnostics and improving their therapeutic potential. In this study, we focused on the expression of miR-21, -96, -196a, -210, and -217 in normal fibroblasts, cancer-associated fibroblasts prepared from a ductal adenocarcinoma of the pancreas, and pancreatic carcinoma cell lines. We compared these data with miRNAs in homogenates of paraffin-embedded sections from normal pancreatic tissues. In cancer-associated fibroblasts and cancer cell lines, miRNAs differed significantly from the normal tissue. In detail, miR-21 and -210 were significantly upregulated, while miR-217 was downregulated. Similar transcription profiles were earlier reported in cancer-associated fibroblasts exposed to hypoxia. However, the cells in our study were cultured under normoxic conditions. We also noted a relation to IL-6 production. In conclusion, cultured cancer-associated fibroblasts and carcinoma cells reflect miR-21 and -210 expression similarly to the cancer tissue samples harvested from the patients.

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Section: Resultsmentioning

confidence: 99%

Expression of Selected miRNAs in Normal and Cancer-Associated Fibroblasts and in BxPc3 and MIA PaCa-2 Cell Lines of Pancreatic Ductal Adenocarcinoma

Mandys

Popov

Gürlich

et al. 2023

IJMS

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“…In the case of miR‐210, Zimmermann et al [ 42 ] described the overexpression of this miRNA in metastasis vs. primary GEP NENs and in samples with higher proliferative rates. Moreover, its role in angiogenesis is also well‐known: miR‐210 levels increases in hypoxia and induce HIF1A and VEGF expression by inhibiting VEGF's negative regulators or through the JAK/STAT pathway [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA signature and integrative omics analyses define prognostic clusters and key pathways driving prognosis in patients with neuroendocrine neoplasms

et al. 2023

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Neuroendocrine neoplasms (NENs) are mutationally quiet (low number of mutations/Mb), and epigenetic mechanisms drive their development and progression. We aimed at comprehensively characterising the microRNA (miRNA) profile of NENs, and exploring downstream targets and their epigenetic modulation. In total, 84 cancer‐related miRNAs were analysed in 85 NEN samples from lung and gastroenteropancreatic (GEP) origin, and their prognostic value was evaluated by univariate and multivariate models. Transcriptomics ( N = 63) and methylomics ( N = 30) were performed to predict miRNA target genes, signalling pathways and regulatory CpG sites. Findings were validated in The Cancer Genome Atlas cohorts and in NEN cell lines. We identified a signature of eight miRNAs that stratified patients in three prognostic groups (5‐year survival of 80%, 66% and 36%). Expression of the eight‐miRNA gene signature correlated with 71 target genes involved in PI3K–Akt and TNFα–NF‐kB signalling. Of these, 28 were associated with survival and validated in silico and in vitro . Finally, we identified five CpG sites involved in the epigenetic regulation of these eight miRNAs. In brief, we identified an 8‐miRNA signature able to predict survival of patients with GEP and lung NENs, and identified genes and regulatory mechanisms driving prognosis in NEN patients.

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“…Among them, miRNA-210 (miR-210) is the established goal of hypoxia inducible factor, and its upregulation is the characteristic of hypoxia condition. At present, most studies on the function of miR-210 have focused on its relationship with various cancers, including pancreatic cancer and lung cancer [6,7]. Studies in infertility diseases have found that miR-210 in testicular tissue rises signi cantly in two types of diseases (the spermatocyte stage (MA) and Sertoli cell-only syndrome (SCOS) ), indicating that miR-210 may play a key role in the meiosis stage of spermatocytes and secretion-related functions in Sertoli cells [8].…”

Section: Introductionmentioning

confidence: 99%

miRNA-210 increased apoptosis of testicular tissues in cryptorchidism mice model through INHBB-Smad2/3 signaling pathway

Wang

et al. 2023

Preprint

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Background Cryptorchidism, as one of the common diseases of genitourinary abnormalities in newborn boys, has become an important factor leading to male infertility in the future. However, the specific pathogenesis remains poorly understood. The present experimental study aimed to clarify the mechanism of spermatogenic dysfunction caused by miR-210 in cryptorchidism. Methods In this study, 16 male ICR mice were classified into cryptorchidism group (n = 8), normal control group (n = 8), and the mice were killed in different age to create cryptorchidism self-control group. Then, the gene expression of miR-210 in testis tissues of three groups of mice, was detected by qPCR. HE staining and Tunel fluorescence staining were used to observe pathological changes and apoptosis of testis in cryptorchidism mice. The protein expression of INHBB was observed by immunohistochemistry. Finally, Western blot was used to detect the related proteins in the INHBB-Smad2/3-Casp3 pathway. Results The results indicated the expression of miR-210 was the most significant difference on the 14th day after cryptorchidism operation. We found that 14 days after the operation, apoptosis in the testis of cryptorchidism mice increased significantly. Finally, we found that the protein expressions of INHBB,Smad2/3, P-Smad2/3, and Caspase3 in the testicular tissues of cryptorchidism mice were significantly increased by detecting cryptorchidism mice with increased expression of miR-210. Conclusion Our results revealed the function of miR-210 and established the regulatory relationship between miR-210 and INHBB, which plays an important role in testicular tissue apoptosis.

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Association of Exosomal miR-210 with Signaling Pathways Implicated in Lung Cancer

Cited by 12 publications

References 149 publications

Expression of Selected miRNAs in Normal and Cancer-Associated Fibroblasts and in BxPc3 and MIA PaCa-2 Cell Lines of Pancreatic Ductal Adenocarcinoma

Expression of Selected miRNAs in Normal and Cancer-Associated Fibroblasts and in BxPc3 and MIA PaCa-2 Cell Lines of Pancreatic Ductal Adenocarcinoma

MicroRNA signature and integrative omics analyses define prognostic clusters and key pathways driving prognosis in patients with neuroendocrine neoplasms

miRNA-210 increased apoptosis of testicular tissues in cryptorchidism mice model through INHBB-Smad2/3 signaling pathway

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