Association of Escherichia coli O157:H7 with necrotizing enterocolitis in a full-term infant

Guner, Yigit S.; Malhotra, Ajay; Ford, Henri R.; Stein, James E.; Kelly, Lisa K.

doi:10.1007/s00383-009-2365-3

Cited by 17 publications

(10 citation statements)

References 23 publications

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“…Recently NEC outbreaks have been linked with norovirus 16–18 . Other studies have linked NEC with Clostridium perfringens 5 , Escherichia coli 6 , Clostridium difficile 4 , rotavirus 11 , astrovirus 9 and others. However, in almost all cases pathogen detection has been performed from stool samples but not from tissue.…”

Section: Discussionmentioning

confidence: 99%

“…Although considered an infectious disease for a long time, no single pathogen has been identified consistently. Reports of NEC have linked this disease with a variety of intestinal pathogens including Clostridium difficile 4 , Clostridium perfringens 5 , Escherichia coli 6 , adenovirus 7–8 , astrovirus 9 , enterovirus 10 , rotavirus 11 , and norovirus 12 . The number of reports of viral pathogens associated with NEC has tripled in the last 5 years and it has been suggested that rotavirus may constitute as much as 30% and norovirus 40% of NEC cases in single center cohorts 3,11–12 .…”

Section: Introductionmentioning

confidence: 99%

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Absence of Gastrointestinal Pathogens in Ileum Tissue Resected for Necrotizing Enterocolitis

Ullrich

Tang²,

Correa

et al. 2012

Pediatric Infectious Disease Journal

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Necrotizing enterocolitis (NEC) is the one of the most common gastrointestinal emergencies in premature infants and has been linked with viral antigens for as much as 40% of cases in single center cohorts. We examined 28 tissue sections from surgically resected ileum from 27 preterm infants with NEC from 2 separate institutions for 15 common bacterial, viral, and parasitic gastrointestinal pathogens using multiplex RT-PCR amplification and suspension array detection methods. We did not detect infectious enteritis pathogens in any of the NEC tissues and conclude that gastrointestinal pathogens are a rare cause of NEC.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Absence of Gastrointestinal Pathogens in Ileum Tissue Resected for Necrotizing Enterocolitis

Ullrich

Tang²,

Correa

et al. 2012

Pediatric Infectious Disease Journal

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“…We further characterized the genotypic variation of E. coli through reconstructing MLST loci-sequences from metagenomes 21 , and compared them to 25 recently characterized E. coli MLST genotypes associated with NEC 22,23 . Five E. coli genotypes were only observed in samples with high IP, two of which, sequence type 73 and 131, were previously identified as uropathogenic E. coli (UPEC) strains associated with NEC and infant mortality 23 ( Supplemental File 6 ).…”

Section: Resultsmentioning

confidence: 99%

Microbial Biomarkers of Intestinal Barrier Maturation in Preterm Infants

McComb

Gajer

et al. 2018

Preprint

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19Intestinal barrier immaturity, or "leaky gut," is the proximate cause of susceptibility to 20 necrotizing enterocolitis in preterm neonates. However, the impact of intestinal 21 microbiota development on intestinal mucosal barrier maturation has not been 22 evaluated in this population. In this study, we investigated a longitudinally sampled 23 cohort of 38 preterm infants monitored for intestinal permeability (IP) and fecal 24 microbiota during the first two weeks of life. Rapid decrease in IP indicating intestinal 25 barrier function maturation correlated with significant increase in community diversity. In 26 particular, members of the Clostridiales and Bifidobacterium were highly 27 transcriptionally active, and progressively increasing abundance in Clostridiales was 28 significantly associated with decreased gut permeability. Further, neonatal factors 29 previously identified to promote intestinal barrier maturation, including early exclusive 30 breastmilk feeding and low antibiotic exposure, favor the early colonization of the gut 31 microbiota by members of the Clostridiales, which altogether are associated with 32 improved intestinal barrier function in preterm infants. 33 affecting approximately 7-10% of preterm neonates with mortality as high as 30-50% 13 . 57In this condition, bacteria across the intestinal wall leading to local and systemic 58 infection and inflammation, and bowel wall necrosis and perforation. Intestinal barrier 59 immaturity, characterized as elevated intestinal permeability (IP), or "leaky gut", is the 60 proximate cause of susceptibility to NEC in preterm neonates 14,15 . It is critical to 61 characterize the preterm infant intestinal microbiota to identify dysbiotic states 62 associated with increased intestinal leakiness, as well as beneficial bacteria associated 63 with improved intestinal barrier function, for subsequent stratification of early diagnosis, 64 early intervention and primary prevention of leaky gut and its sequelae. 65 66Despite the critical role of the microbial community in intestinal barrier function, its effect 67 on newborn IP is unknown. In particular, the microbiota of preterm neonates with 68 measured elevated IP, a high-risk population for NEC, has not been studied previously. 69We hypothesize that the intestinal microbiota plays a pivotal role in modulating IP and 70 that the presence of "beneficial" bacteria will be associated with improved intestinal 71 barrier function in preterm infants. In this study, we studied a cohort of 38 preterm 72 infants born prior to 33 weeks of gestation. IP was measured by urinary detection of 73 orally administered sugar probes lactulose and rhamnose using high pressure liquid 74 chromatography 16 with coinciding measures of the composition and function of the fecal 75 microbial communities were investigated. We sampled three time points, study day 1, 8, 76and 15, during the first two weeks of life, which is a critical period corresponding to the 77 initiation of the intestinal microbiota development process [16][17]...

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“…However, no specific microbe has been identified as determinant etiologic factor, and, rather surprisingly, the specific mechanisms by which infections contribute to NEC remain unknown. On the other hand, many pathogens may simulate a picture of NEC in neonates (Table 1) [7,[25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44]. The use of new molecular biology techniques has provided opportunities to reexamine this unresolved problem.…”

Section: Gut Microbiotamentioning

confidence: 99%

Update in Pathogenesis and Prospective in Treatment of Necrotizing Enterocolitis

Terrin

Scipione

Curtis

2014

BioMed Research International

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Necrotizing enterocolitis (NEC) is among the most common and devastating diseases in neonates and, despite the significant advances in neonatal clinical and basic science investigations, its etiology is largely understood, specific treatment strategies are lacking, and morbidity and mortality remain high. Improvements in the understanding of pathogenesis of NEC may have therapeutic consequences. Pharmacologic inhibition of toll-like receptor signaling, the use of novel nutritional strategies, and microflora modulation may represent novel promising approaches to the prevention and treatment of NEC. This review, starting from the recent acquisitions in the pathogenic mechanisms of NEC, focuses on current and possible therapeutic perspectives.

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Association of Escherichia coli O157:H7 with necrotizing enterocolitis in a full-term infant

Cited by 17 publications

References 23 publications

Absence of Gastrointestinal Pathogens in Ileum Tissue Resected for Necrotizing Enterocolitis

Absence of Gastrointestinal Pathogens in Ileum Tissue Resected for Necrotizing Enterocolitis

Microbial Biomarkers of Intestinal Barrier Maturation in Preterm Infants

Update in Pathogenesis and Prospective in Treatment of Necrotizing Enterocolitis

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