Association of Escherichia coli HEp-2 adherence patterns with type and duration of diarrhoea

Cravioto, Alejandro; Tello, Angel; Navarro, Armando; Ruiz, Joaquı́m; Villafán, H.; Uribe, F. Medardo Tapia; Eslava, Carlos

doi:10.1016/0140-6736(91)90868-p

Cited by 318 publications

(269 citation statements)

References 18 publications

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“…The EAggEC have been recognized as a cause of persistent diarrhea in HIVinfected infants in countries that have common borders with the Central African Republic 31 and in several other countries. [32][33][34] In the Central African Republic, EAggEC strains were found only in HIVϩ immunodeficient adults who had no other enteric pathogen and should be considered an opportunistic agent whenever isolated from diarrhea stools of AIDS patients. Conversely, more caution seems indicated for interpreting the pathogenic role of E. coli with localized or diffuse adherence phenotype encountered in this study.…”

Section: Discussionmentioning

confidence: 99%

“…34 According to other studies, these observations point to the existence of different mechanisms by which EAggEC strains may cause disease exist. 29,33,35 These isolates are under investigation for their epidemiologic features. The importance of coccidia as a widespread cause of diarrhea is now increasing.…”

Section: Discussionmentioning

confidence: 99%

“…33 Six strains harbored a phenotype (cell-detaching E. coli) representing a putative new enteric virulent category of E. coli previously identified in Australia. 34 According to other studies, these observations point to the existence of different mechanisms by which EAggEC strains may cause disease exist.…”

Section: Discussionmentioning

confidence: 99%

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Etiologies of acute, persistent, and dysenteric diarrheas in adults in Bangui, Central African Republic, in relation to human immunodeficiency virus serostatus.

Germani¹,

Minssart²,

Vohito³

et al. 1998

The American Journal of Tropical Medicine and Hygiene

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Abstract. A study of the etiologies of diarrhea in adults in relation to their human immunodeficiency virus (HIV) serostatus and number of CD4ϩ cells was carried out in the Central African Republic. In cases and controls, multiparasitism was observed. Salmonella spp. were identified mainly during acute diarrhea, with 50% of the S. enteritidis isolated during the study being responsible for septicemia and/or urinary tract infection in immunodeficient patients. Enteroaggregative Escherichia coli (EAggEC) were the most frequently identified agent in HIVϩ patients with persistent diarrhea; 42.8% of the patients with EAggEC as sole pathogens had bloody diarrhea, and these strains were negative for the presence of a virulence plasmid. Coccidia were found in those with acute and persistent diarrhea. Blood was observed in 53.3% of infections involving coccidia as the sole pathogen. Microsporidium spp. and Blastocystis hominis were found only in HIVϩ patients with persistent diarrhea. Shigella spp., Campylobacter spp., and Entamoeba histolytica were found in HIVϩ and HIVϪ dysenteric patients; bacteria resembling spirochetes that could not be cultivated were identified only in HIVϩ cases with dysentery. Shiga-like toxin-producing E. coli O157:HϪ was isolated from two cases with hemolytic-uremic syndrome. Fungi were identified as the sole pathogen in 6.4% of the HIVϩ patients with persistent diarrhea. Most of enteropathogenic bacteria identified were resistant to ampicillin and trimethoprim-sulfamethoxazole, remained susceptible to ampicillin plus clavulanic acid, and were susceptible to amikacin, gentamicin, and ciprofloxacin.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Etiologies of acute, persistent, and dysenteric diarrheas in adults in Bangui, Central African Republic, in relation to human immunodeficiency virus serostatus.

Germani¹,

Minssart²,

Vohito³

et al. 1998

The American Journal of Tropical Medicine and Hygiene

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“…Enteroaggregative Escherichia coli (EAggEC) is a class of diarrheagenic E. coli associated with diarrhea in both epidemiologic and volunteer studies (2,3,13,33,34). EAggEC is defined by its characteristic aggregative adherence (AA) to HEp-2 cells in cell culture (33).…”

mentioning

confidence: 99%

AggR, a transcriptional activator of aggregative adherence fimbria I expression in enteroaggregative Escherichia coli

et al. 1994

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Enteroaggregative Escherichia coli (EAggEC) has been associated with persistent pediatric diarrhea in the developing world, yet the pathogenetic mechanisms of EAggEC infection are unknown. Our previous data have suggested that aggregative adherence of some EAggEC strains to HEp-2 cells is mediated by flexible, bundle-forming fimbriae, which we have termed aggregative adherence fimbriae I (AAF/I). Genes sufficient to confer expression of AAF/I are located on the 60-MDa plasmid of EAggEC 17-2; AAF/I genes are present as two unlinked plasmid regions (regions 1 and 2), separated by 9 kb of DNA. Here we report the complete DNA sequencing of region 2 and the identification of an open reading frame which is involved in the expression of AAF/I. One open reading frame of 794 bp encodes a protein (designated AggR) with a predicted molecular size of 29.4 kDa, which shows a high degree of amino acid sequence identity to CfaR and other members of the AraC class of gene regulators. The cloned aggR gene (or, alternatively, a cloned cfaR gene) was sufficient to complement a region 1 clone to confer AAF/I expression. To further substantiate the role of aggR in the regulation of AAF/I, we constructed a 289-bp in-frame aggR deletion and replaced the native gene in 17-2 by allelic exchange, using the temperature-sensitive vector pIB307. The resulting aggR deletions were negative for AAF/I expression, but expression was restored when the aggR gene (cloned into pBluescript II SK) was reintroduced into the aggR mutant. RNA slot blot experiments using a probe for the putative AAF/I pilin subunit (aggA) revealed that aggR operates as a transcriptional activator of aggA expression. aggA::phoA fusions were constructed in 17-2 and in 17-2 delta aggR. AggR was found to promote expression of the aggA gene under a variety of conditions of temperature, osmolarity, oxygen tension, and medium. At acid pH, aggA expression was maximal and was regulated by both AggR-dependent and AggR-independent mechanisms.

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“…Although most E. coli are non-pathogenic in the intestine, some can produce diarrhea by a number of distinct mechanisms (15) . Enteropathogenic E. coli (EPEC) has been one of the causative agents of infantile diarrhea, which has continued to be a major health problem in developing countries (1,2). EHEC has emerged to be an important enteric pathogen as the main cause of hemorrhagic colitis and the epidemic form of hemolytic uremic syndrome, especially in developed countries (11,18) .…”

mentioning

confidence: 99%

Development of an Enzyme‐Labeled Oligonucleotide Probe for Detecting the Escherichia coli Attaching and Effacing A Gene

Nagayama

Akeda

et al. 1999

Microbiology and Immunology

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EnteropathogenicEscherichia coli (EPEC) and enterohemorrhagic Escherichia coli (EHEC ) can produce attaching and effacing (AE) lesions on intestinal epithelium in vitro and in vivo. A gene necessary to cause the AE lesion has been identified and designated Escherichia coli attaching and effacing A (eaeA) gene. In this study, an alkaline phosphatase (ALP)-conjugated oligonucleotide probe for the eaeA gene was developed and used to detect the eaeA gene among 163 strains of classical EPEC and 25 strains of EHEC The prevalence rates of eaeA gene in the strains of classical EPEC and EHEC O157 were 51.5 and 100%, respectively. The eaeA-positive rate (60.0 %) in strains of class I EPEC serogroups (O26, O55, O86 ,

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Association of Escherichia coli HEp-2 adherence patterns with type and duration of diarrhoea

Cited by 318 publications

References 18 publications

Etiologies of acute, persistent, and dysenteric diarrheas in adults in Bangui, Central African Republic, in relation to human immunodeficiency virus serostatus.

Etiologies of acute, persistent, and dysenteric diarrheas in adults in Bangui, Central African Republic, in relation to human immunodeficiency virus serostatus.

AggR, a transcriptional activator of aggregative adherence fimbria I expression in enteroaggregative Escherichia coli

Development of an Enzyme‐Labeled Oligonucleotide Probe for Detecting the Escherichia coli Attaching and Effacing A Gene

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