Association of Enzymes with Replicating and Nonreplicating Simian Virus 40 Chromosomes

Virion-mediated transfer of SV40 epigenetic information

“…For these studies, nuclei were prepared according to our standard protocol, which was originally optimized for the yield of biologically active SV40 minichromosomes. 26 As shown in Figure 2B, a significantly smaller percentage of infecting SV40 chromatin was found in the nucleus following infection with cs1085 compared with wild-type virus at 30 min PI indicating that the epigenetic information carried by the two viruses had an effect on import into the nucleus. The epigenetic information carried by the infecting virions also had an effect on cellular degradation of the SV40 chromatin.…”

Separation of replicating simian virus 40 chromosomes according to extent of replication

“…Proliferating cell nuclear antigen (PCNA) has been shown (Prelich el a/., 1 9 8 7~ ;Bravo et al, 1987) recently to be one of the cellular proteins required for efficient viral DNA replication. appear to be identical: both occur predominantly bidirectionally, make Okazaki pieces of the same size, use the same size and type of RNA primer and respond to inhibitors of DNA synthesis in a similar fashion (Edenberg & Huberman, 1975;Tseng & Goulian, 1977;Edenberg et al, 1978;Waqar et al, 1978;Tsubota et al, 1979;Waqar eta/., 1983). The question as to whether PCNA factor (or DNA polymerase 6 auxiliary protein) is associated with replicating SV40 chromosomes, remains to be investigated in the future.…”

“…The question as to whether PCNA factor (or DNA polymerase 6 auxiliary protein) is associated with replicating SV40 chromosomes, remains to be investigated in the future. , Waqar et al, 1983;Waqar, 1988) and short chains are converted to long chains (Edenberg et al, 1977;Su & DePamphilis, 1978;Tsubota et al, 1979;Waqar, 1988 Su & DePamphilis, 1978;Tsubota et al, 1979;Waqar, 1988). Replicating SV40 chromosomes can be extracted from the nuclei of infected green monkey kidney cells (CV 1) and are ca able of synthesizing DNA in the presence of [acells, extensive DNA synthesis occurs (Edenberg et al, 1977;Su & DePamphilis, 1978; Tsubota e t a / .…”

“…DNA polymerase a-like activity has been detected in abundance in sucrose or glycerol gradient fractions containing replicating SV40 chromosomes (Edenberg et al, 1978;Tsubota et al, 1979;Waqar et al, 1983). This finding does not rule out the involvement of b-like polymerase for the limited DNA synthesis in vitro which is independent of exogenous template (Waqar et al, 1983;Waqar, 1988).…”

Association of Type I DNA Topoisomerase with Herpes Simplex Virus

“…In both prokaryotes and eukaryotes these enzymes appear ubiquitous and have been isolated from a variety of sources (Gellert, 1981;Liu, 1983). Topoisomerases have also been identified in vaccinia virus cores (Bauer et al, 1977) and simian virus 40 (SV40) minichromosomes (Tsubota et al, 1978).…”