Association of Ehrlichia canis, Hemotropic Mycoplasma spp. and Anaplasma platys and severe anemia in dogs in Thailand

Kaewmongkol, Gunn; Lukkana, Nicha; Yangtara, Sarawut; Kaewmongkol, Sarawan; Thengchaisri, Naris; Sirinarumitr, Theerapol; Jittapalapong, Sathaporn; Fenwick, Stanley G.

doi:10.1016/j.vetmic.2017.01.022

Cited by 45 publications

(49 citation statements)

References 36 publications

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“…Mycoplasma haemocanis causes hemolytic anemia in immunocompromised dogs (KAEWMONGKOL et al, 2017). The first record of this pathogen in Mexico was in 1960, and detected in splenectomized dogs (OSORNO & RISTIC, 1972).…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of Bartonella, Ehrlichia and Mycoplasma in feral dogs of El Pedregal de San Angel Ecological Reserve in Mexico City

Arenas

Gil-Alarcón

Sánchez‐Montes

et al. 2019

Rev. Bras. Parasitol. Vet.

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Free-ranging and feral dogs represent a group of unattended companion animals. They impact wild animal populations by predating native species, displacing predators and introducing exotic pathogens. The aim of this work was to describe the molecular occurrence of Rickettsia, Ehrlichia, Anaplasma, Mycoplasma and Bartonella in feral dogs. The study was carried out in the last relict of a protected area in Mexico City. Blood clots samples from 19 dogs were obtained and analyzed for detection of specific fragments of the 16S-rRNA gene for Anaplasma, Ehrlichia and Mycoplasma and citrate synthase (gltA) for Bartonella and Rickettsia. Our results showed that DNA from three bacteria species (Bartonella vinsonii subsp. berkhoffii, Ehrlichia canis and Mycoplasma haemocanis) was present with frequencies ranging from 5.3 to 15.8%. This is the first record of B. vinsonii subsp. berkhoffii and M. haemocanis in dogs from México, and also the first finding of Ehrlichia canis in Mexico City. It is important to perform surveillance of feral dog populations in order to identify the impact of these pathogens on wild animal populations and Public Health in order to establish prevention and protection programs.

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Section: Discussionmentioning

confidence: 99%

Molecular detection of Bartonella, Ehrlichia and Mycoplasma in feral dogs of El Pedregal de San Angel Ecological Reserve in Mexico City

Arenas

Gil-Alarcón

Sánchez‐Montes

et al. 2019

Rev. Bras. Parasitol. Vet.

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“…In particular, countries spanning the tropics must affront an expansive range of CVBDs that comprise a leading cause of fatality in dogs [3][4][5][6]. Bacterial infections can be some of the deadliest CVBDs in such regions with pathogens such as Ehrlichia canis, the causative agent of canine monocytic ehrlichiosis (CME), being the biggest contributor to a raft of life-threatening conditions, including pancytopenia, fever, bleeding tendencies and immunosuppression [7][8][9]. Other disease-causing species include, Anaplasma platys which is a cause of recurrent thrombocytopenia in canines [10], haemotropic mycoplasmas that are associated with haemolytic syndrome [6] and Bartonella spp.…”

Section: Introductionmentioning

confidence: 99%

“…that can produce severe endocarditis [11]. Rates of canine infection with such pathogenic agents can be very high, especially in tropical countries of Southeast Asia, where, for example 25.5% of Malaysian, 21.8% of Cambodian and 9.9% of Thai dogs have previously been found positive for E. canis by PCR [3,6,8]. Lower but nonetheless significant levels of infection by haemotropic mycoplasmas (3.7-12.8%) and A. platys (3.7-4.4%) have also been detected in these same countries [6,8].…”

Section: Introductionmentioning

confidence: 99%

A Host-Specific Blocking Primer Combined with Optimal DNA Extraction Improves the Detection Capability of a Metabarcoding Protocol for Canine Vector-Borne Bacteria

et al. 2020

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Bacterial canine vector-borne diseases are responsible for some of the most life-threatening conditions of dogs in the tropics and are typically poorly researched with some presenting a zoonotic risk to cohabiting people. Next-generation sequencing based methodologies have been demonstrated to accurately characterise a diverse range of vector-borne bacteria in dogs, whilst also proving to be more sensitive than conventional PCR techniques. We report two improvements to a previously developed metabarcoding tool that increased the sensitivity and diversity of vector-borne bacteria detected from canine blood. Firstly, we developed and tested a canine-specific blocking primer that prevents cross-reactivity of bacterial primer amplification on abundant canine mitochondrial sequences. Use of our blocking primer increased the number of canine vector-borne infections detected (five more Ehrlichia canis and three more Anaplasma platys infections) and increased the diversity of bacterial sequences found. Secondly, the DNA extraction kit employed can have a significant effect on the bacterial community characterised. Therefore, we compared four different DNA extraction kits finding the Qiagen DNeasy Blood and Tissue Kit to be superior for detection of blood-borne bacteria, identifying nine more A. platys, two more E. canis, one more Mycoplasma haemocanis infection and more putative bacterial pathogens than the lowest performing kit.

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“…Overall, 26/176 (14.8%) positive ticks were coinfected with two pathogens. An increasing number of studies report that ticks and their vertebrate hosts often harbour multiple infections, suggesting that this might be the rule rather than the exception (Belongia 2002;Swanson et al 2006;Ginsberg 2008;Raileanu et al 2017), and hosts infected with several pathogens may show more severe symptoms of diseases (Krause 2002;Pañczuk et al 2016;Kaewmongkol et al 2017).…”

Section: Tick-borne Pathogensmentioning

confidence: 99%

Some aspects on tick species in Mongolia and their potential role in the transmission of equine piroplasms, Anaplasma phagocytophilum and Borrelia burgdorferi L.

et al. 2018

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Ticks are cosmopolitan vectors of numerous diseases, and detection of various pathogens in ticks can help to assess their distribution. In the current study, 528 adult ticks were collected from grazing animals or the ground in ten different Mongolian provinces. Dermacentor nuttalli constituted 76.1% of them and was found in all ecozones except the eastern desert. Dermacentor marginatus (8.3%), Dermacentor silvarum (1.1%) and Ixodes persulcatus (3.0%) were found in the northern forest areas and Hyalomma asiaticum (11.4%) only in the southern (semi-)desert. Of these, 359 ticks were subjected to DNA extraction and PCR was carried out to detect various pathogens. Anaplasma spp. was found in D. marginatus and D. nuttalli (2.5% positive each), including flagged specimen and identified as Anaplasma phagocytophilum. Borrelia spp. were found in 2.5% of the ticks (mostly in I. persulcatus) and identified as Borrelia garinii. Babesia spp. (40%) identified as Babesia caballi were detected in all five tick species including flagged Dermacentor spp. and I. persulcatus, and 3.5% of the ticks (all species except D. silvarum) were positive for Theileria spp. identified as Theileria equi. The piroplasms were found in all provinces. Tick-borne encephalitis virus was not detected. The results highlight the high risk of equine piroplasmosis in Mongolia, which is a concern for both the nomadic population who rely on horses for transport and for conservation of Przewalski's horses in Mongolia. In addition, zoonotic agents such as the avian B. garinii and A. phagocytophilum were also detected, outlining a high risk for exposed humans.

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Association of Ehrlichia canis, Hemotropic Mycoplasma spp. and Anaplasma platys and severe anemia in dogs in Thailand

Cited by 45 publications

References 36 publications

Molecular detection of Bartonella, Ehrlichia and Mycoplasma in feral dogs of El Pedregal de San Angel Ecological Reserve in Mexico City

Molecular detection of Bartonella, Ehrlichia and Mycoplasma in feral dogs of El Pedregal de San Angel Ecological Reserve in Mexico City

A Host-Specific Blocking Primer Combined with Optimal DNA Extraction Improves the Detection Capability of a Metabarcoding Protocol for Canine Vector-Borne Bacteria

Some aspects on tick species in Mongolia and their potential role in the transmission of equine piroplasms, Anaplasma phagocytophilum and Borrelia burgdorferi L.

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