Association of cytokine gene polymorphisms with chronic hepatitis C virus genotype 1b infection in Chinese Han population

Abstract

Cytokines are extensively involved in the process of hepatitis C virus (HCV) infection and take a crucial part in host immune regulation. We aimed to explore the potential correlation of cytokine single nucleotide polymorphisms (SNPs) with HCV susceptibility and response rate of interferon (IFN)-based antiviral therapy in Chinese Han population. A case–control genetic association study was conducted between 198 patients with chronic HCV genotype 1b infection and 142 healthy controls. Genetic polymorphisms of TNF-α (rs1800629), TGF-β (rs1800469), IL-10 (rs1800896, rs1800871, and rs1800872), IL-6 (rs1800795, rs1800796), IFN-γ (rs2430561), and IL-28B (rs12979860, rs12980275, and rs8099917) were analyzed by MassARRAY SNP technology. Patients were treated with IFNα-2b or pegylated-IFNα-2a plus ribavirin for 48 weeks. Sustained virological response (SVR) was assessed 6 months after the completion of the treatment. The IL-28B rs12979860-CC (odds ratio [OR] = 4.35, 95% confidence interval [CI]: 1.69–11.21, P = .001), rs12980275-AA (OR = 3.41, 95% CI: 1.08–10.76, P = .028), and rs8099917-TT (OR = 3.86, 95% CI: 1.49–10.12, P = .004) were significantly associated with SVR, and IL-10 rs1800871-TT (OR = .50, 95% CI: 0.25–1.00, P = .049) and rs1800872-AA (OR = .50, 95% CI: 0.25–1.00, P = .049) were also significant for SVR. No association was found between the cytokine SNPs and HCV susceptibility. Additionally, multivariate analysis showed that low baseline viral load (OR = 3.63, 95% CI: 1.01–13.02, P = .048), pegylated-IFN (OR = 9.68, 95% CI: 1.14–82.13, P = .037) and rs12979860-CC (OR = 6.08, 95% CI: 2.00–18.46, P = .001) were independent factors for SVR. IL-28 and IL-10 gene polymorphisms played an important role in predicting host response to IFN-based antiviral therapy in HCV genotype 1b infection.

Cytokine (IL-10, IL-6, TNF-α and TGF-β1) Gene Polymorphisms in Chronic Hepatitis C Virus Infection among Malay Male Drug Abusers

“…However, these findings are in contrast to several other studies. A study conducted on chronic HCV infection patients in a Chinese Han population did not observe any significant association between the gene polymorphism of IL-10 rs1800871 and susceptibility to HCV infection [18]. Conversely, they found that the gene polymorphism of rs1800871 played a significant role in patients' response towards antiviral therapy.…”

Impact of IL-10 gene promoter polymorphisms on treatment response in HCV patients: A systematic review, a meta-analysis, and a meta-regression

“…Overall, 32 studies with a total of 5943 HCV cases (NR: 3380; SVR: 2563) and 2697 controls were included in the present study. 4 – 8 , 17 – 43 Included studies characteristics are summarized in Table 4 . Twenty-three studies were included for the rs1800896 (-1082 A/G) SNP, 4 – 8 , 17 , 19 , 22 – 26 , 29 – 37 , 39 , 41 17 for the rs1800871 (-819 C/T) SNP, 4 – 7 , 19 , 22 ,…”

“… 4 – 8 , 17 – 43 Included studies characteristics are summarized in Table 4 . Twenty-three studies were included for the rs1800896 (-1082 A/G) SNP, 4 – 8 , 17 , 19 , 22 – 26 , 29 – 37 , 39 , 41 17 for the rs1800871 (-819 C/T) SNP, 4 – 7 , 19 , 22 , 27 , 30 …”

Identificación de los polimorfismos rs12979860 y rs8099917 de IL28B en el diagnóstico, control y determinación de tratamiento en pacientes venezolanos infectados con el virus de hepatitis C.

“…Otro factor que condiciona la respuesta al tratamiento y evolución de las enfermedades, es el factor genético (17). Estudios realizados en China establecieron que el gen IL28B representa un factor predictivo importante en la respuesta a tratamientos basados con IFN en pacientes infectados con el VHC 1b (18). Öksüz y col., reportaron una relación estadísticamente significativa entre el gen IL28B y el receptor tipo Toll 2 (TLR2) en pacientes infectados con VHC con manifestaciones de fibrosis; concluyendo que juntos son biomarcadores predictivos de enfermedad severa en pacientes con VHC (19).…”