Association of cytokeratin 17 expression with differentiation in oral squamous cell carcinoma

Abstract

PurposeThe aim of this study was to confirm the expression profile of cytokeratin (CK)17 in comparison with that of CK13 in oral squamous cell carcinoma (OSCC) and leukoplakia and to clarify an association of CK17 with the OSCC differentiation.MaterialsThe expression of CK17 and CK13 was immunohistochemically examined in 105 patients with OSCC and 108 patients with leukoplakia. A correlation of CK expression with clinicopathological variables was carried out. The over-expression levels of CK17 mRNA were analyzed by real-time RT-PCR in 5 OSCC cell lines (HSC-2, HSC-3, SAS, SQUU-A, SQUU-B).ResultsCK17 and CK13 were detected in 101 (96.2 %) and three (2.9 %) of the 105 OSCCs, respectively. CK17 was significantly expressed in well-differentiated OSCC compared to moderately/poorly differentiated OSCC (p < 0.01). As detected in 19 of the 34 dysplastic leukoplakias (55.9 %) and 36 of the 74 hyperplastic leukoplakias (48.6 %), CK17 was significantly expressed in dysplastic leukoplakias (p < 0.01). As detected in 11 of the 34 dysplastic (32.4 %) and 52 of the 74 hyperplastic leukoplakias (70.3 %), CK13 was significantly expressed in hyperplastic leukoplakias (p < 0.01). The relative expression of CK17 mRNA in HSC-2 was significantly higher than in HSC-3 and SAS (p < 0.05). Moreover, the relative expression of CK17 mRNA in SQUU-A was significantly higher than in SQUU-B (p < 0.05).ConclusionCK17 expression could be associated with the differentiation and the malignancy of OSCC. A combination pattern of CK17/CK13 might be a suitable marker of malignant transformation.

Lack of Utility of Cytokeratins in Differentiating Pseudocarcinomatous Hyperplasia of Granular Cell Tumors from Oral Squamous Cell Carcinoma

“…Although many studies have claimed that CK13 (-)/CK17 (+) is the unique profile pattern of oral SCC (Mikami, Cheng et al 2011 ;Kitamura, Toyoshima et al 2012 ), we showed that CKs pattern for GCT-PCH is CK13 (-) /CK17 (+). CK is just a one example of the possible IHC similarity between PCH and oral SCC.…”

Keratins 17 and 19 expression as prognostic markers in oral squamous cell carcinoma

“…Confirmation of these results was obtained by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) (Toyoshima et al, 2009). Higher expression of K17 has also been reported in patients with stage I and II OSCC and in well-differentiated oral cavity carcinoma (Toyoshima et al, 2009;Kitamura et al, 2012).…”

“…Our results show that these keratins are highly expressed in tumoral tissue compared to non-tumoral surgical margins. Other studies have shown the presence of K17 in gastric adenocarcinoma, Ewing sarcoma, OSCC, and ovary, breast, and cervical cancers (Maddox et al, 1999;Ide et al, 2012;Kitamura et al, 2012;Alshareeda et al, 2013;Sankar et al, 2013;Wang et al, 2013). K19 expression was shown in liver, breast, lung, ovary, thyroid and oral cancers (Safadi et al, 2010;Isic Dencic et al, 2013;Kong et al, 2013;Liu et al, 2013;Park et al, 2013).…”

“…Keratins 17 and 19 (K17 and K19) are type I keratins and their varying expression patterns have been implicated in cancer. Prognostic associations between K17 and K19 have been made for gastric adenocarcinoma, breast cancer, intraepithelial cervical cancer, ovarian cancer, hepatocellular carcinoma, and lung, larynx, thyroid,and oral cancer (Cohen-Kerem et al, 2004;Ikeda et al, 2008;Safadi et al, 2010;Ide et al, 2012;Kitamura et al, 2012;Alshareeda et al, 2013;Bugalho et al, 2013;Kaczka et al, 2013;Lee et al, 2013;Wang et al, 2013). In the present study, we evaluated the differential expression of K17 and K19 in tumoral and non-tumoral tissue.…”

Quantification of (&ndash;)-Epigallocatechin-3-gallate Inhibition of Anaplastic Thyroid Cancer Cell Line Adhesion and Proliferation Using Real-time Cell Analysis

“…TCO-1 JCRB0239 cells were established from a human nondifferentiated thyroid cancer 17 , the SAS JCRB0260 cell line was established from poorly differentiated OSCC of the tongue 18 , and the HO-1-u-1 cell line JCRB0828 was established from a poorly differentiated OSCC of the oor of the mouth 19 . EGCG MW458.37 was purchased from Wako Pure Chemical Industries Osaka, Japan .…”