Association of Class 1 and 2 Integrons with Multidrug-Resistant Acinetobacter baumannii International Clones and Acinetobacter nosocomialis Isolates

Martins, Natacha; Picão, Renata C.; Adams‐Sapper, Sheila; Riley, Lee W.; Moreira, Beatriz Meurer

doi:10.1128/aac.02415-14

Cited by 28 publications

(24 citation statements)

References 23 publications

(35 reference statements)

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“…In addition to the genes responsible for carbapenem resistance and high MIC values for tigecycline, most of the strains analyzed by MLST belonged to CC113, confirming its dissemination in Brazil and Latin America [ 12 , 63 , 13 – 14 , 64 , 15 – 16 ]. Additionally, a new ST, ST1399, belonging to CC229, is reported.…”

Section: Discussionmentioning

confidence: 91%

Molecular characterization and clonal dynamics of nosocomial blaOXA-23 producing XDR Acinetobacter baumannii

et al. 2018

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The emergence of infections associated to new antimicrobial resistance in Acinetobacter baumannii (Ab) genotypes represents a major challenge. In this context, this study aimed to determine the diversity of resistance mechanisms and investigate clonal dissemination and predominant sequence types (STs) in multidrug-resistant Ab strains of clinical (tracheal aspirate, n = 17) and environmental (surface, n = 6) origins. Additionally, the major clones found in clinical (A) and environmental (H) strains had their complete genomes sequenced. All strains were submitted to polymerase chain reactions (PCR) for the detection of the ISAba1/blaOXA-51-like and ISAba1/blaOXA-23-like genes, while the expression of genes encoding the carO porin, AdeABC (adeB), AdeFGH (adeG), and AdeIJK (adeJ) efflux pumps was determined by real time PCR (qPCR). Most of the strains were characterized as extensively drug-resistant (XDR) with high minimal inhibitory concentrations (MICs) detected for tigecycline and carbapenems. Associations between ISAba1/OXA-51 and ISAba1/OXA-23 were observed in 91.3% and 52.2% of the strains, respectively. Only the adeB gene was considered hyper-expressed. Furthermore, most of the strains analyzed by the MuLtilocus Sequence-Typing (MLST) were found to belong to the clonal complex 113 (CC113). In addition, a new ST, ST1399, belonging to CC229, was also discovered herein. Strains analyzed by whole genome sequencing presented resistance genes linked to multidrug-resistance phenotypes and confirmed the presence of Tn2008, which provides high levels carbapenem-resistance.

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Section: Discussionmentioning

confidence: 91%

Molecular characterization and clonal dynamics of nosocomial blaOXA-23 producing XDR Acinetobacter baumannii

et al. 2018

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“…Also, Kamalbeik et al showed that 7.5% and 67.5% of the strains contained class I and II integrons, respectively [23]. In the studies carried out by Ramírez et al [24] and Martins et al [25], the prevalence of class II Integron was 23% and 41.7%, respectively. In the research conducted by Taherikalani et al (Tehran at 2012) [26] and Moammadi et al (west of Iran at 2012) [27] the frequency of class I, II, and III integrons was 85%, 14%, and 0%, and 97%, 31%, and 0%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Integron-Mediated Antibiotic Resistance inAcinetobacter baumanniiIsolated from Intensive Care Unit Patients, Babol, North of Iran

Salehi

Ferdosi-Shahandashti

Yahyapour

et al. 2017

BioMed Research International

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Background We investigated the integron types and their relation with antibiotic resistance among A. baumannii isolates collected from intensive care unit patients, Babol, north of Iran. Methods In this cross-sectional study, a total of 73 bronchoalveolar lavage samples were obtained from patients in ICU. Susceptibility testing was performed by disk diffusion method. Types of integrons were identified by an integrase gene PCR. Results In total, 47.9% A. baumannii isolates were recovered from the BAL samples. All isolates were resistant to ceftazidime. 91.4% and 58.3% of isolates were MDR and XDR, respectively. The rate of colistin resistance with the E-test was 5.7%. Molecular analysis of class I, II, and III integrons showed that 25.7%, 88.6%, and 28.6% of the isolates carried the intI, intII, and intIII genes, respectively. Discussion Our results show that different classes of integrons are commonly spread among A. baumannii strains and these genomic segments can play an important role in the acquisition of MDR and XDR phenotypes. So monitoring drug resistance in A. baumannii isolates with the use of int gene PCR is very important to plan specific infection control measures to prevent the spread of MDR-AB and XDR-AB in Iran's hospitals.

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“…Interestingly, Martins et al 7 showed the same distribution of class 1 and 2 integrons, related to specific CCs such as CC15 and CC79. 7 In a previous study, we reported a high prevalence of class 2 integrons in southern Brazil. Indeed, these results might be explained by the association of class 2 integrons to CC79, which are prevalent in South America.…”

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confidence: 94%

Emergence of OXA-72-producing Acinetobacter baumannii Belonging to High-Risk Clones (CC15 and CC79) in Different Brazilian States

Pagano

Rocha

Sampaio

et al. 2016

Infect. Control Hosp. Epidemiol.

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The addition of an easy-to-use qualitative component to hand hygiene compliance observations and consecutive training efforts is important, given that <10% of all hand disinfections were performed correctly in an observational study by Tschudin-Sutter et al, 1 who observed the 6-step technique. Appropriate hand-surface coverage was reached in only 7.9% of hand hygiene procedures observed by Park et al, 2 despite a high rate of compliance with the correct indications. Shah et al 3 performed a video observation of hand washing. Of 1,081 recordings, 403 (37.3%) were excellent, 521 (48.2%) were acceptable, and 157 (14.5%) were unacceptable.A limitation of our study is the lack of bacterial counts, but the results of Riley et al, 4 who showed no correlation between hand coverage and bacterial counts with a 6-step technique compared to a 3-step approach, had not been published at the time of our experiment. 4 Another limitation is the small number of participants and the experimental setting of this proof-of-principle study. However, we believe that based on our results, the addition of dichotomous subjective quality assessment using the parameters time and skin coverage during live observation by experienced infection control staff is feasible and could be a valuable addition to conventional hand hygiene observation.acknowledgments

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Association of Class 1 and 2 Integrons with Multidrug-Resistant Acinetobacter baumannii International Clones and Acinetobacter nosocomialis Isolates

Cited by 28 publications

References 23 publications

Molecular characterization and clonal dynamics of nosocomial blaOXA-23 producing XDR Acinetobacter baumannii

Molecular characterization and clonal dynamics of nosocomial blaOXA-23 producing XDR Acinetobacter baumannii

Integron-Mediated Antibiotic Resistance inAcinetobacter baumanniiIsolated from Intensive Care Unit Patients, Babol, North of Iran

Emergence of OXA-72-producing Acinetobacter baumannii Belonging to High-Risk Clones (CC15 and CC79) in Different Brazilian States

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