Association of CiaRH with resistance of <i>Streptococcus mutans</i> to antimicrobial peptides in biofilms

Mazda, Yusuke; Kawada‐Matsuo, Miki; Kanbara, Kenji; Oogai, Yuichi; Shibata, Yukie; Yamashita, Yoshihisa; Miyawaki, Shouichi; Komatsuzawa, Hitoshi

doi:10.1111/j.2041-1014.2012.00637.x

Cited by 32 publications

(26 citation statements)

References 38 publications

(82 reference statements)

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“…In our study, 3 of the 15 TCSs identified in S. mutans were demonstrated to be associated with resistance to human- or bacteria-derived antimicrobial peptides [55], [59]. Furthermore, other researchers reported that 3 additional TCSs were associated with production of or resistance to antibacterial agents [48], [60], [61].…”

Section: Discussionsupporting

confidence: 52%

“…As a result of this comprehensive analysis, one TCS ( ciaRH ) knockout mutant showed increased susceptibility to LL37 and beta-defensins (HBD1-3) in biofilm cells [55]. Further experiments showed that CiaRH regulates the dlt operon, which plays an important role in the acquisition of resistance to positively-charged antimicrobial agents, such as LL37 and β-defensins.…”

Section: Association Of Tcs With Antimicrobial Susceptibility In S Mmentioning

confidence: 90%

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Role of Streptococcus mutans two-component systems in antimicrobial peptide resistance in the oral cavity

Kawada‐Matsuo

Komatsuzawa

2017

Japanese Dental Science Review

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SummaryApproximately 100 trillion microorganisms exist in the oral cavity. For the commensal bacteria of the oral cavity, it is important to adapt to environmental stimuli, including human- or bacteria-derived antimicrobial agents. Recently, bacterial-specific signal transduction regulatory systems, called two-component systems (TCSs), which appear to be focused on sensing and adapting to the environment, were discovered. Streptococcus mutans is an oral commensal bacteria and is also known as a cariogenic bacteria. Although the virulence factors of S. mutans have been well demonstrated, the mechanism underlying the adaptation of the species to the oral cavity is poorly understood. S. mutans UA159 has 15 sets of TCSs. Among them, several have been demonstrated to be involved in acid tolerance, competence and biofilm formation. Recently, together with our findings, it was demonstrated that 5 TCSs were involved in resistance to antimicrobial agents. Furthermore, another TCS was associated with the production of bacteriocin. Six of 15 TCSs are associated with antimicrobial agents, implying that S. mutans can survive in the oral cavity by resisting various antimicrobial peptides.In this review, we highlight the role of antimicrobial peptides in the oral cavity.

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Section: Discussionsupporting

confidence: 52%

Section: Association Of Tcs With Antimicrobial Susceptibility In S Mmentioning

confidence: 90%

Role of Streptococcus mutans two-component systems in antimicrobial peptide resistance in the oral cavity

Kawada‐Matsuo

Komatsuzawa

2017

Japanese Dental Science Review

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“…The primers used are shown in Table 2. Since the DNA gyrase A subunit (gyrA) was stably expressed and used as the internal control for quantitative PCR (32,33), the amount of gyrA was used as an internal control in this study. Three independent experiments were performed, and the means Ϯ standard deviations (SD) were calculated.…”

Section: Methodsmentioning

confidence: 99%

dpr and sod in Streptococcus mutans Are Involved in Coexistence with S. sanguinis, and PerR Is Associated with Resistance to H ₂ O ₂

Fujishima

Kawada‐Matsuo

Oogai

et al. 2013

Appl Environ Microbiol

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Large numbers of bacteria coexist in the oral cavity. Streptococcus sanguinis, one of the major bacteria in dental plaque, produces hydrogen peroxide (H 2 O 2 ), which interferes with the growth of other bacteria. Streptococcus mutans, a cariogenic bacterium, can coexist with S. sanguinis in dental plaque, but to do so, it needs a means of detoxifying the H 2 O 2 produced by S. sanguinis. In this study, we investigated the association of three oxidative stress factors, Dpr, superoxide dismutase (SOD), and AhpCF, with the resistance of S. sanguinis to H 2 O 2 . The knockout of dpr and sod significantly increased susceptibility to H 2 O 2 , while the knockout of ahpCF had no apparent effect on susceptibility. In particular, dpr inactivation resulted in hypersensitivity to H 2 O 2 . Next, we sought to identify the factor(s) involved in the regulation of these oxidative stress genes and found that PerR negatively regulated dpr expression. The knockout of perR caused increased dpr expression levels, resulting in low-level susceptibility to H 2 O 2 compared with the wild type. Furthermore, we evaluated the roles of perR, dpr, and sod when S. mutans was cocultured with S. sanguinis. Culturing of the dpr or sod mutant with S. sanguinis showed a significant decrease in the S. mutans population ratio compared with the wild type, while the perR mutant increased the ratio. Our results suggest that dpr and sod in S. mutans are involved in coexistence with S. sanguinis, and PerR is associated with resistance to H 2 O 2 in regulating the expression of Dpr.

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“…The susceptibility of S. mutans to AMPs has been reported to be regulated by dltC gene expression in biofilm cells [65]. …”

Section: Antibiofilm Peptide Applications In Cariologymentioning

confidence: 99%

Antibiofilm peptides against oral biofilms

Wang

Shen

Haapasalo

2017

Journal of Oral Microbiology

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The oral cavity is a major entry point for bacteria and other microorganisms. Oral biofilms are formed by mixed communities of microorganisms embedded in an exopolysaccharide matrix. Biofilms forming on dental hard or soft tissue are the major cause of caries and endodontic and periodontal disease. Human oral biofilms exhibit high resistance to antimicrobial agents. Antibiofilm peptides constitute a diverse class of host-defense molecules that act to combat invasion and infection with biofilms. Different in vitro and in vivo biofilm models with quantitative analysis have been established to provide predictable platforms for the evaluation of the antibiofilm effect of oral antibiofilm peptides. These peptides have engendered considerable interest in the past decades as potential alternatives to traditional disinfecting agents due to their ability to target bacterial biofilms specifically, leading to the prevention of biofilm formation and destruction of pre-existing biofilms by Gram-positive and -negative bacterial pathogens and fungi. At the same time, challenges associated with the application of these antibiofilm peptides in dental practice also exist. The production of effective, nontoxic, and stable antibiofilm peptides is desired in both academic and industrial fields. This review focuses on the antibiofilm properties of current synthetic peptides and their application in different areas of dentistry.

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Association of CiaRH with resistance of Streptococcus mutans to antimicrobial peptides in biofilms

Cited by 32 publications

References 38 publications

Role of Streptococcus mutans two-component systems in antimicrobial peptide resistance in the oral cavity

Role of Streptococcus mutans two-component systems in antimicrobial peptide resistance in the oral cavity

dpr and sod in Streptococcus mutans Are Involved in Coexistence with S. sanguinis, and PerR Is Associated with Resistance to H ₂ O ₂

Antibiofilm peptides against oral biofilms

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Association of CiaRH with resistance of Streptococcus mutans to antimicrobial peptides in biofilms

Cited by 32 publications

References 38 publications

Role of Streptococcus mutans two-component systems in antimicrobial peptide resistance in the oral cavity

Role of Streptococcus mutans two-component systems in antimicrobial peptide resistance in the oral cavity

dpr and sod in Streptococcus mutans Are Involved in Coexistence with S. sanguinis, and PerR Is Associated with Resistance to H 2 O 2

Antibiofilm peptides against oral biofilms

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Product

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dpr and sod in Streptococcus mutans Are Involved in Coexistence with S. sanguinis, and PerR Is Associated with Resistance to H ₂ O ₂