Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence

Cavalcante, José Roosevelt; Pinheiro, Luiz Gonzaga Porto; Almeida, Paulo Roberto Carvalho de; Ferreira, Márcia Valéria Pitombeira; Cruz, Gabriela Silva; Campelo, Thales Alves; Silva, Caroliny Soares; Lima, Luana Nepomuceno Gondim da Costa; Oliveira, Bruno Masato Kitagawa De; Lima, Lara Mulato; Feitosa, Laura Magda Costa; Pinheiro, Agostinho Câmara; Frota, Cristiane Cunha

doi:10.6061/clinics/2018/e465

Cited by 23 publications

(14 citation statements)

References 37 publications

(75 reference statements)

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“…There is a range of discrepancies in the literature concerning the HPV prevalence in breast cancer patients. In line with our data, investigations involving cases and controls indicate a higher frequency of HPV in tumor tissues (37)(38)(39)(40)(41), while there are some studies that reported contradictory data (42,43). The distribution of the HPV genotype in this research indicated that all detected types belonged to high-risk HPV.…”

Section: Discussionsupporting

confidence: 88%

Downregulation of miR-143/145 Cluster in Breast Carcinoma Specimens: Putative Role of DNA Oncoviruses

Nakhaie

Makvandi

Charostad

et al. 2021

Jundishapur J Microbiol

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Background: The DNA oncoviruses, including human papillomavirus (HPV) and Epstein-Barr virus (EBV) are among the most important infectious agents involved in breast carcinogenesis. These oncoviruses have a broad disrupting effect on cellular miRNAs and their functions, by which they contribute to carcinogenesis. Objectives: In this investigation, we evaluated the correlation between HPV and EBV and the expression level of tumor suppressor miRNAs (miR-143 and 145), clinical outcomes, and their association with stimulating inflammatory cytokines in patients with breast carcinoma. Methods: In our case-control study, 35 cancerous tissues and 35 adjacent non-cancerous tissues were collected from 35 patients. Nested-PCR was set up for the detection of HPV and EBV genomes, and RT-qPCR was used for miRNA expression in the case and control groups. In addition, serum specimens were obtained from all patients (n = 35) and healthy controls (n = 35) to determine the IL-8 serum concentration. Results: We found HPV and EBV in 14.2% (10/70) and 7% (5/70) of all samples, respectively. The distributions of positive samples in the case and control groups were 25.7% (9/35) and 2.9% (1/35) (P = 0.006) for HPV and 11.4% (4/35) and 2.9% (1/35) (P = 0.164) for EBV, respectively. Besides, RT-qPCR showed that miR-143 and miR-145 were significantly downregulated in HPV and EBV-infected cases compared to non-infected ones (P < 0.05). Data also indicated that the promotion of metastasis status was related to miR-143/145 downregulation and HPV infection (P = 0.003). No significant difference was found in serum IL-8 concentration concerning viral infections. Conclusions: Our results suggested the possible involvement of viral infections in breast carcinogenesis and adverse clinical outcomes by downregulating miR-143/145.

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Section: Discussionsupporting

confidence: 88%

Downregulation of miR-143/145 Cluster in Breast Carcinoma Specimens: Putative Role of DNA Oncoviruses

Nakhaie

Makvandi

Charostad

et al. 2021

Jundishapur J Microbiol

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“…While a solid link between HPV and HNC (including anogenital cancers) has been made, a link between HPV and some cases of breast cancers is still debatable/controversial. As mentioned earlier, HPV16 has been detected in the breast milk from a mother and in oral samples from her spouse [38,40]; furthermore, an increasing number of studies have also detected HPV DNA and the expression of E7 protein in breast cancer samples in Europe [102,103], North America [104], Central/South America [105,106], and Asia/Oceania [107,108,109]. Thus, there is increasing data and it is likely that HPV may be associated with some types of breast cancers.…”

Section: Prospects For the Futurementioning

confidence: 99%

A Current Update on Human Papillomavirus-Associated Head and Neck Cancers

Tumban

2019

Viruses

148

138

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Human papillomavirus (HPV) infection is the cause of a growing percentage of head and neck cancers (HNC); primarily, a subset of oral squamous cell carcinoma, oropharyngeal squamous cell carcinoma, and laryngeal squamous cell carcinoma. The majority of HPV-associated head and neck cancers (HPV + HNC) are caused by HPV16; additionally, co-factors such as smoking and immunosuppression contribute to the progression of HPV + HNC by interfering with tumor suppressor miRNA and impairing mediators of the immune system. This review summarizes current studies on HPV + HNC, ranging from potential modes of oral transmission of HPV (sexual, self-inoculation, vertical and horizontal transmissions), discrepancy in the distribution of HPV + HNC between anatomical sites in the head and neck region, and to studies showing that HPV vaccines have the potential to protect against oral HPV infection (especially against the HPV types included in the vaccines). The review concludes with a discussion of major challenges in the field and prospects for the future: challenges in diagnosing HPV + HNC at early stages of the disease, measures to reduce discrepancy in the prevalence of HPV + HNC cases between anatomical sites, and suggestions to assess whether fomites/breast milk can transmit HPV to the oral cavity.

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“…For the rst time in 1992 Di Lonardo A and colleagues suggested that HPV could play a crucial role in the genesis of breast carcinoma by detecting HPV16 DNA sequence in 29.4% of breast and lymph node samples (28). Following the rst publication, several more studies have documented the presence of HPV in malignant breast tumors (29)(30)(31). On the contrary negative relationship between HPV and breast cancer has been declared by few studies (32)(33)(34).…”

Section: Introductionmentioning

confidence: 99%

Detection of Human Papillomavirus Genotypes in Breast Cancer Patients

Mofrad

Sadigh

Ainechi

et al. 2020

Preprint

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Background: The role of viruses as a cause of breast cancer (BC) have been significantly investigated in recent years. Human papillomavirus (HPV) has been detected in invasive breast carcinomas, while most studies have only focused on the detection of viral DNA, we aimed to determine genotypes of the culprit HPV's. Methods: We collected and analyzed 70 Formalin Fixed Paraffin Embedded (FFPE) blocks including 59 BC samples, and 11 benign breast lesions as control from Iranian patients using nested PCR. Real-time PCR utilized as a confirming test to nested PCR findings. Genotyping of HPV positive samples was performed, the samples were also subjected to a multiplex PCR to detect herpes simplex-1 (HSV-1), herpes simplex-2 (HSV-2), varicella zoster virus (VZV) and cytomegalovirus (CMV) in BC.Results: Papillomavirus DNA was present in 7 of 59 BC samples (11.8%); while none was detected in control samples. The most prevalent type was HPV18, followed by HPV 6. No HPV16 genotype was detected .All HPV positive patients had high tumor grades (II/ III) with histologic diagnosis of ductal carcinoma. The patient age range was 33 to 73 years with median of 51 years. Most of HPV positive patients had low levels of education. Also 5 of 59 BC specimens (8.47%), were positive for HSV-1.Conclusions: Our results suggest a carcinogenesis role for High risk HPVs in breast tumors. Our findings of HPV6 and HSV1 genotypes in BC samples, propose a cancer causing role for low risk HPVs and herpes viruses. Further large scale studies are warranted to assess the significance of our findings.

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Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence

Cited by 23 publications

References 37 publications

Downregulation of miR-143/145 Cluster in Breast Carcinoma Specimens: Putative Role of DNA Oncoviruses

Downregulation of miR-143/145 Cluster in Breast Carcinoma Specimens: Putative Role of DNA Oncoviruses

A Current Update on Human Papillomavirus-Associated Head and Neck Cancers

Detection of Human Papillomavirus Genotypes in Breast Cancer Patients

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