Association of Biofilm Production of Coagulase-Negative Staphylococci with Expression of a Specific Polysaccharide Intercellular Adhesin

Mack, Dietrich; Haeder, Michael; Siemssen, Nicolaus; Laufs, Rainer

doi:10.1093/infdis/174.4.881

Cited by 134 publications

(87 citation statements)

References 13 publications

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“…The CoNS strains (n ϭ 200) belong to a collection of clinical isolates which were consecutively collected at the University Hospital HamburgEppendorf in 1997 and 1998 and include 13 different species (140 S. epidermidis, 16 S. haemolyticus, 10 S. hominis, 9 S. saprophyticus, 6 S. capitis, 4 S. lugdunensis, 4 S. warneri, 4 S. xylosus, 2 S. schleiferi, 2 S. cohnii, 1 S. chromogenes, 1 S. simulans, and 1 S. kloosii isolates), as described previously (13,28). S. aureus ATCC 29213 (mecA negative) and S. aureus ATCC 43300 (mecA positive) were used for quality control.…”

Section: Methodsmentioning

confidence: 99%

Rapid Detection of Methicillin Resistance in Coagulase-Negative Staphylococci with the VITEK 2 System

et al. 2002

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The aim of the present study was to evaluate the accuracy of the new VITEK 2 system (bioMérieux, Marcy l' Etoile, France) for the detection of methicillin resistance in coagulase-negative staphylococci (CoNS) by using AST-P515 and AST-P523 test cards. Analyses of the VITEK 2 oxacillin MIC determination evaluated according to the actual breakpoint (>0.5 g/ml) of the National Committee for Clinical Laboratory Standards resulted in a high sensitivity of 99.2% but a moderate specificity of 80%. The newly included oxacillin resistance (OR) test of the VITEK 2 system displayed a high sensitivity and a high specificity of 97.5 and 98.7%, respectively. Concordance between the results of the mecA PCR and the VITEK 2 oxacillin MIC was observed for almost all Staphylococcus epidermidis strains, but the reduced specificity was attributable to higher oxacillin MICs for mecA-negative non-S. epidermidis strains, especially S. saprophyticus, S. lugdunensis, and S. cohnii. Evaluation of alternative oxacillin MIC breakpoints of 1, 2, or 4 g/ml resulted in improved degrees of specificity of 84, 90.7, and 97.3%, respectively. Only minor changes occurred in the corresponding sensitivity values, which were 98.4, 97.5, and 97.5%, respectively. Methicillin resistance in CoNS was detected after 7 and 8 h in 91.1 and 93.5% of the mecA-positive strains, respectively, by the VITEK 2 OR test and in 86.3 and 89.5% of the mecA-positive strains, respectively, by VITEK 2 oxacillin MIC determination. After 7 and 8 h the VITEK 2 OR test classified 59.2 and 78.9% of the mecA-negative strains, respectively, as susceptible to oxacillin, whereas comparable values were obtained 2 h later by VITEK 2 oxacillin MIC determination. The results of our study encourage the use of the VITEK 2 system, which proved to be a highly reliable and rapid phenotypic method for the detection of methicillin resistance in CoNS.

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Section: Methodsmentioning

confidence: 99%

Rapid Detection of Methicillin Resistance in Coagulase-Negative Staphylococci with the VITEK 2 System

et al. 2002

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“…Secretion of the exopolysaccharide PIA is a major factor that determines biofilm formation in S. epidermidis and other bacteria (18,30,31). To test whether the altered biofilm formation that we observed in the ⌬luxS mutant strain was due to a change in the transcription of the ica operon responsible for PIA production (21), we analyzed transcription of the icaC gene by quantitative real-time PCR.…”

Section: )mentioning

confidence: 99%

Role of theluxSQuorum-Sensing System in Biofilm Formation and Virulence ofStaphylococcus epidermidis

et al. 2006

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Nosocomial infections caused by Staphylococcus epidermidis are characterized by biofilm formation on implanted medical devices. Quorum-sensing regulation plays a major role in the biofilm development of many bacterial pathogens. Here, we describe luxS, a quorum-sensing system in staphylococci that has a significant impact on biofilm development and virulence. We constructed an isogenic ⌬luxS mutant strain of a biofilmforming clinical isolate of S. epidermidis and demonstrated that luxS signaling is functional in S. epidermidis. The mutant strain showed increased biofilm formation in vitro and enhanced virulence in a rat model of biofilm-associated infection. Genetic complementation and addition of autoinducer 2-containing culture filtrate restored the wild-type phenotype, demonstrating that luxS repressed biofilm formation through a cell-cell signaling mechanism based on autoinducer 2 secretion. Enhanced production of the biofilm exopolysaccharide polysaccharide intercellular adhesin in the mutant strain is presumably the major cause of the observed phenotype. The agr quorum-sensing system has previously been shown to impact biofilm development and biofilm-associated infection in a way similar to that of luxS, although by regulation of different factors. Our study indicates a general scheme of quorum-sensing regulation of biofilm development in staphylococci, which contrasts with that observed in many other bacterial pathogens.

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“…The second phase is characterized by bacterial accumulation, which requires cell-to-cell interaction. The intercellular polysaccharide adhesin (PIA) has been found to mediate intercellular adhesion in the majority of clinical S. epidermidis isolates (13,35,41,60). This linear glucosaminoglycan is composed of N-acetylglucosamine in ␤-1,6-glycosidic linkages containing deacetylated amino groups and succinate and phosphate substituents (34,36).…”

mentioning

confidence: 99%

Glucose-Related Dissociation between icaADBC Transcription and Biofilm Expression by Staphylococcus epidermidis : Evidence for an Additional Factor Required for Polysaccharide Intercellular Adhesin Synthesis

et al. 2003

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Biofilm formation in Staphylococcus epidermidis depends, in the majority of the strains, on the activity of the icaADBC locus. The expression of the operon that encodes the synthetic enzymes of the intercellular polysaccharide adhesin (PIA) depends on a variety of exogenic environmental conditions and is, at least in part, regulated by the alternative sigma factor B . We investigated the transcriptional regulation of the ica operon and the respective phenotypes expressed under growth conditions differing in the content of glucose in the growth medium. In the presence of glucose, S. epidermidis exhibited a PIA-and biofilm-positive phenotype whereas ica transcription was down-regulated in the postexponential and stationary phases of growth. Surprisingly, maximum transcription of ica was detectable in the stationary phase of growth in the absence of glucose despite the expression of a PIA-and biofilm-negative phenotype. In vitro enzymatic assays and phenotypic characterization showed that the abundant amount of ica mRNA was functionally active because induction of stationary-phase cells with glucose led to immediate PIA synthesis. Induction of biofilm formation could be completely inhibited by chloramphenicol, which, given at a later stage of biofilm accumulation, also inhibited further development of preformed biofilm, indicating that continuous translation of an additional, icaADBC-independent factor is required for the expression of a biofilm-positive phenotype.

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Association of Biofilm Production of Coagulase-Negative Staphylococci with Expression of a Specific Polysaccharide Intercellular Adhesin

Cited by 134 publications

References 13 publications

Rapid Detection of Methicillin Resistance in Coagulase-Negative Staphylococci with the VITEK 2 System

Rapid Detection of Methicillin Resistance in Coagulase-Negative Staphylococci with the VITEK 2 System

Role of theluxSQuorum-Sensing System in Biofilm Formation and Virulence ofStaphylococcus epidermidis

Glucose-Related Dissociation between icaADBC Transcription and Biofilm Expression by Staphylococcus epidermidis : Evidence for an Additional Factor Required for Polysaccharide Intercellular Adhesin Synthesis

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