Association of Antibiotic Resistance, Cell Adherence, and Biofilm Production with the Endemicity of NosocomialKlebsiella pneumoniae

Abstract

Klebsiella pneumoniae is a leading cause of multiple nosocomial infections, some of which are associated with high mortality. The increasing prevalence of antibiotic-resistant strains highlights their clinical importance and how complicated managing treatment can be. In this study, we investigated antimicrobial resistance, cell adherence, and biofilm production of nosocomial K. pneumoniae strains isolated from surveillance studies in a Mexican tertiary hospital and evaluated the potential association of these phenotypes with endemicity. The great majority of the clones exhibited adhesion to cultured epithelial cells and were strong biofilm producers. A direct relationship between adhesion phenotypes, biofilm production, and endemicity was not always apparent. Biofilm formation and production of ESBL did not appear to be directly associated. Notably, all the endemic strains were multidrug-resistant. This study emphasizes that while endemic strains possess various virulence-associated properties, antimicrobial resistance appears to be a determining factor of their endemicity.

Genotypic characterisation and antimicrobial resistance of Pseudomonas aeruginosa strains isolated from patients of different hospitals and medical centres from Poland

“…Inappropriate empirical therapy is perceived as the main factor contributing to increased mortality [26]. Detection and elimination of dissemination of high-risk clones in many cases is not possible with the use of simple epidemiological data alone [11,27]. In this study, we demonstrate the presence of endemic strains at ICU and burn units that were not detected by hospital stuff.…”

Antibody-recruiting protein-catalyzed capture agents to combat antibiotic-resistant bacteria

“…These rods are large extracellular structures (0.5-2 mm long, 4-to-5 nm in diameter) 54,55 that are each comprised of up to 1000s of MrkA copies. 56 The selection of target epitopes on MrkA is illustrated in Fig. 2B.…”

“…CH1034_10002 corresponds to phnV). Finally, we manually reviewed available information 54,56,[73][74][75][76][77][78][79][80] about genes present in both sets to select a target, prioritizing gene targets that yielded virulence-associated proteins that were either membrane-spanning or oriented on the extracellular side of the outer membrane.…”

Antibody-Recruiting Protein-Catalyzed Capture Agents to Combat Antibiotic-Resistant Bacteria

“…These rods are large extracellular structures (0.5-2 µm long, 4-to-5 nm in diameter) (48,49) that are each comprised of up to 1,000s of MrkA copies. (50) The selection of target epitopes on MrkA is illustrated in Figure 2B. Selection of surface exposed epitopes would be aided by protein structure, but no published structures exist for MrkA.…”

“…CH1034_10002 corresponds to phnV). Finally, we manually reviewed available information (48,50,(64)(65)(66)(67)(68)(69)(70)(71) about genes present in both sets to select a target, prioritizing gene targets that yielded virulence-associated proteins that were either membrane-spanning or oriented on the extracellular side of the outer membrane.…”