Association of Alpha Tocopherol and Ag Sulfadiazine Chitosan Oleate Nanocarriers in Bioactive Dressings Supporting Platelet Lysate Application to Skin Wounds

Abstract

Chitosan oleate was previously proposed to encapsulate in nanocarriers some poorly soluble molecules aimed to wound therapy, such as the anti-infective silver sulfadiazine, and the antioxidant α tocopherol. Because nanocarriers need a suitable formulation to be administered to wounds, in the present paper, these previously developed nanocarriers were loaded into freeze dried dressings based on chitosan glutamate. These were proposed as bioactive dressings aimed to support the application to wounds of platelet lysate, a hemoderivative rich in growth factors. The dressings were characterized for hydration capacity, morphological aspect, and rheological and mechanical behavior. Although chitosan oleate nanocarriers clearly decreased the mechanical properties of dressings, these remained compatible with handling and application to wounds. Preliminary studies in vitro on fibroblast cell cultures demonstrated good compatibility of platelet lysate with nanocarriers and bioactive dressings. An in vivo study on a murine wound model showed an accelerating wound healing effect for the bioactive dressing and its suitability as support of the platelet lysate application to wounds.

New Nanotechnologies for the Treatment and Repair of Skin Burns Infections

“…The authors reported that CS-OA and αTph NEs promote cell proliferation in keratinocytes and fibroblast cell cultures. The study suggested that CS-OA and αTph are appropriate for topical application in wound healing [57].…”

Chitosan Oleate Salt as an Amphiphilic Polymer for the Surface Modification of Poly-Lactic-Glycolic Acid (PLGA) Nanoparticles. Preliminary Studies of Mucoadhesion and Cell Interaction Properties

“…Chitosan oleate maintained in this case an antimicrobial effect, in accordance with literature findings [ 20 , 21 ]. Nanoemulsions based on alpha tocopherol and stabilized by means of chitosan oleate salt were studied on fibroblast and keratinocyte cell lines, on ex vivo human biopsies, and in vivo on a rat burn model, showing the biological effects of both chitosan and oleic acid on the wound healing promotion [ 22 , 23 ].…”

Platelet Lysate-Derived Neuropeptide y Influences Migration and Angiogenesis of Human Adipose Tissue-Derived Stromal Cells

“…Finally, in order to confirm that the employment of PL-based preparations in vivo could foster an increase of NPY, we have focused on chronic wounds of patients that exhibit impaired healing and where PL is successfully employed as regenerative tool to enhance skin repair and local angiogenesis 14 , 23 , 41 , 42 . Specifically, we have selected human biopsy samples obtained from the edge of lesion of difficult wounds (defined as hard-to-heal or chronic wounds), which previously have been clinically treated with the autologous leuco-platelet concentrate, a PL-like preparation.…”