Association of ALOX15 gene polymorphisms with obesity-related phenotypes in Chinese nuclear families with male offspring

Abstract

Aim: Genetic variation in ALOX12, which encoded human 12-lipoxygenase, was found to be associated with fat mass in young Chinese men. The objective of this study was to investigate the relationship between single nucleotide polymorphisms (SNPs) and haplotypes in the ALOX15 gene and obesity-related phenotypes in Chinese nuclear families with male offspring. Methods: We recruited 1,296 subjects from 427 nuclear families with male offspring and genotyped five SNPs (rs9894225, rs748694, rs2619112, rs2619118, and rs916055) in the ALOX15 gene locus. The total fat mass (TFM), trunk fat mass (tFM), leg fat mass (LFM) and arm fat mass (AFM) were measured using dual-energy X-ray absorptiometry (DXA). The percentage of fat mass (PFM) was the ratio of TFM and body weight. The association between SNPs and haplotypes of ALOX15 and obesity-related phenotypic variation was measured using quantitative transmission disequilibrium test (QTDT). Results: Using QTDT to measure family-based genetic association, we found that rs916055 had a statistically significant association with PFM (P=0.038), whereas rs916055 had a marginal but statistically insignificant association with tFM (P=0.093). The multipleparameter 1000 permutations test agreed with the family-based association results: both showed that rs916055 had a statistically significant association with PFM (P=0.033). Conclusion: rs916055 in ALOX15 gene was significantly associated with the percentage of fat mass in Chinese nuclear families with male offspring in the family-based association study using QTDT approach.

Polymorphisms in inflammation associated genes ALOX15 and IL-6 are associated with bone properties in young women and fracture in elderly

“…Epidemiological studies have reported associations, albeit inconsistent, between genetic variation in both the ALOX12 and ALOX15 genes and bone properties [15--20], and with body Page 4 of 20 composition and fat mass [21,22]. Strengthening their putative role as candidate genes for osteoporosis, ALOX12/15 metabolites can act as ligands for peroxisome proliferator--activated receptor gamma (PPARγ) [23].…”

LGR4 Gene Polymorphisms Are Associated With Bone and Obesity Phenotypes in Chinese Female Nuclear Families

“…Each enrolled subject was asked to answer questions concerning their demographic characteristics, lifestyle, dietary habits, and tobacco/alcohol use. The study participant exclusion criteria were as follows: (1) significant health problems due to cerebral vascular disease; (2) diabetes; (3) chronic kidney, liver, or lung disease; (4) alcoholism; (5) pharmacologic doses of corticosteroids for >3 months; (6) anticonvulsant therapy for >6 months; (7) evidence of other hereditary or metabolic bone diseases; (8) rheumatoid arthritis or a collagen disease; (9) major gastrointestinal disease within the last year; (10) any major endocrinopathy that would affect bone mass such as hyperthyroidism, hypogonadism, or hypercortisolism; (11) any neurological or musculoskeletal condition that might cause secondary low bone mass (16,(24)(25)(26)(27)(28)(29)(30).…”