Association of ACE, FABP2 and GST genes polymorphism with essential hypertension risk among a North Indian population

Abstract

It is concluded that ACE (rs 4646994), FABP2 (rs1799883) and GST (GSTM1 null or positive genotype and GSTT1 null or positive genotype) genes polymorphism are associated with HTN. Further investigation with a larger sample size may be required to validate this study.

Association of Angiotensin-Converting Enzyme and Glutathione S-Transferase Gene Polymorphisms with Body Mass Index among Hypertensive North Indians

“…The frequencies of the GSTT1-and GSTM1-null genotypes among hypertensive patients in the current study (14.69% and 34.78%, respectively) were lower in comparison to findings from Arab (30.00% and 60.00%, respectively), Chinese (58.10% and 60.00%, respectively) and Korean (55.40% and 51.80%, respectively, and 61.20% and 62.40%, respectively) populations, but similar to findings from another North Indian study (11.59% and 34.05%, respectively). 15,[33][34][35][36] In comparison, the GSTM1-positive genotype was found in 65.22% of the hypertensives in the current study; this was higher than the results from Arab (40.00%), Chinese (40.00%) and Korean (48.20% and 37.60%) populations, but again similar to results from a North Indian study.…”

“…The insertion/insertion genotype frequency in the combined North Indian hypertensive population of the current study (28.07%) was similar to that found in hypertensive Pakistanis (29.30%) and another North Indian population (28.62%). 15,25 There was a significant association between the ACE insertion/insertion genotype and hypertension; this contrasts with the results of a study conducted among South Indians, although it is similar to that of the other study on North Indians. 15,26 The current study found an association between the ACE deletion/deletion genotype and hypertension, although this was not significant.…”

“…15,25 There was a significant association between the ACE insertion/insertion genotype and hypertension; this contrasts with the results of a study conducted among South Indians, although it is similar to that of the other study on North Indians. 15,26 The current study found an association between the ACE deletion/deletion genotype and hypertension, although this was not significant. The frequency of this genotype among hypertensives in the current study (17.18%) was higher than that found for Malaysian and Bangladeshi individuals (10.76% and 11.40%, respectively); however, a Chinese population had a lower frequency.…”

“…[27][28][29] The insertion and deletion alleles were significantly related to hypertension in the current study, which correlates with the findings of a similar North Indian study. 15 A positive correlation has been reported between various obesity variables like BMI and WHR, including oxidative stress markers such as reduced erythrocyte glutathione. 30 In the current study, significant variation was found between the GSTT1-null genotype frequency among the hypertensive overweight/ obese and control groups.…”

“…15 PCR amplification was conducted in a total volume of 20 µL using: 10 pmol each of forward primer 5'-CT-GGAGACCACTCCCATCCTTTCT-3' and reverse primer 5'-GATGTGGCCATCTTCGTCAGAT-3'; 200 ng of genomic DNA; 3 mM of magnesium chloride; 50 mM of potassium chloride; 10 mM of trisaminomethane hydrochloride at a pH of 8.4; 0. 5 15 Primers for GSTM1 were 5'-AACTCCCTGAAAAGCTAAAGC-3' and 5'-GTT-GGGCTCAAATATACGGTGG-3' , while primers for GSTT1 were 5'-TTCCTTACTGGTCCTCACATCT C-3' and 5'-TCACCGGACATGGCCAGCA-3' . To avoid false-negative readings, β-globin was used as an internal control.…”