Association of −604G/A and −501A/C Ghrelin and Obestatin Prepropeptide Gene Polymorphisms with Polycystic Ovary Syndrome

Abstract

Ghrelin hormone has an important role in a wide range of metabolic and non-metabolic processes. Polymorphisms of ghrelin gene could be associated with a large number of diseases. The aim of this study was to evaluate the association of -604G/A and -501A/C polymorphisms in ghrelin and obestatin prepropeptide gene (GHRL) with polycystic ovary syndrome (PCOS) in a sample of Iranian women. One hundred and fifty-two women with PCOS and 162 age-matched apparently healthy women as control group were enrolled in this study. The study subjects were genotyped for polymorphisms in the ghrelin gene using polymerase chain reaction-restriction fragment length polymorphism-based methods. Biochemical parameters, serum prolactin, luteinizing hormone, follicle stimulating hormone, estradiol, and testosterone were estimated by chemiluminescence assay. Serum lipids and lipoproteins were determined by standard enzymatic methods. The association between the risk of PCOS and ghrelin gene polymorphisms was examined using Multivariate analysis. The frequency of the -604G/A and -501A/C polymorphisms was not statistically different between patients and the control group of women (p = 0.12 and p = 0.21, respectively). A significantly higher level of LDL-C was found in the wild-type AA genotype compared with CC genotype of -501A/C polymorphism (p = 0.02). Our findings indicate that neither -604G/A and nor -501A/C polymorphisms of ghrelin gene are associated with PCOS, but suggest a relation between the presence of polymorphic allele of -501A/C polymorphism and LDL-C level in a sample of Iranian women.

Association Between Ghrelin Gene Polymorphism and Cerebral Infarction

“…The protein encoded by the ghrelin gene is the ligand for the growth hormone secretagogue receptor, which mainly regulates the concentration of growth hormone in the body and affects some of the body’s functions through affecting the release of growth hormone, such as regulating growth and development, affecting sugar, lipid and protein metabolism, and relaxing the cardiovascular system [ 16 , 17 ]. It has been proved that the ghrelin gene polymorphisms can affect the occurrence and susceptibility to a variety of diseases, such as polycystic ovary syndrome [ 18 ], chronic hepatitis C [ 19 ], and type 2 diabetes mellitus [ 20 ]. In cerebral infarction patients, the effects of ghrelin gene polymorphisms have not been studied yet.…”

Genetic polymorphisms associated with polycystic ovary syndrome among Iranian women

“…Because there is reportedly an association between these abnormalities and GHRL polymorphisms, the prevalence of such disorders might be related to variations of the GHRL gene. However, there is no evidence of a significant association between PCOS and the –604G/A or –501A/C polymorphism of the GHRL gene in the Iranian population 22 …”

“…However, there is no evidence of a significant association between PCOS and the -604G/A or -501A/C polymorphism of the GHRL gene in the Iranian population. 22…”

Molecular studies on preproghrelin gene: alternative splicing, obestatin effects, coding region and variants

“…However, that peptide clearly shows an important role in metabolism involved with anti-inflammatory and cardioprotective effects [7][8][9][10][11][12][13][14][15][16][17][18][19]. Furthermore, polymorphisms found in GHRL gene are also being associated with different clinical conditions [20][21][22][23]. Thus, this article intends to describe examples of variants found in the GHRL coding gene obestatin-associated and to report the variants produced by alternative splicing processes in the whole gene.…”