Association mechanism between a series of rodenticide and humic acid: A frontal analysis to support the biological data

André, Carl; Guyon, Catherine; Thomassin, Mireille; Barbier, A.; Richert, Lysiane; Guillaume, Yves-Claude

doi:10.1016/j.jchromb.2005.02.020

Cited by 18 publications

(4 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Concerning the 5 rodenticide molecules, and five known ligands of HA, their calculated apparent K d values were consistent with values reported in the literature determined by zonal analysis [43]. The same order of the apparent dissociation K d values described in the literature was observed for this series of rodenticides (K d values from [43]) were given in brackets: Bromadiolone (16.60 μM), brodifacoum (17.70 μM), difenacoum (19.10 μM), chlorophacinone (20.90 μM), diphacinone (23.50 μM). All these results confirmed the good applicability of our analytical method.…”

Section: Binding Constant Values Determined By Frontal Analysissupporting

confidence: 87%

New liquid chromatography columns for highlighting the interaction of ligand candidates with humic acid

Guillaume

André²

2023

J of Separation Science

View full text Add to dashboard Cite

Humic acid was the main compound in soil and reduced the availability of some organic compounds in soils. In this work, humic acid was immobilized for the first time on a homemade neutravidin poly(glycidyl methacrylate-co-ethylene dimethacrylate) capillary column with a 20 μm i.d. for the screening of potential ligands to humic acid and the evaluation of their molecular recognition mechanism. This homemade humic acid column enabling it to work at very low backpressure (0.60 MPa at 20 nl/min flow rate), had a long lifetime, excellent repeatability, and negligible non-specific binding sites. The performance of this affinity humic acid column was demonstrated by the evaluation of recognition assay for a series of known ligands of humic acid (a series of rodenticide molecules) which is the heart of the fragment-based drug design. In addition, this column was used successfully for highlighting the binding mechanism to humic acid of the severe acute respiratory syndrome coronavirus-2-spike protein.As well this new humic acid miniaturized liquid chromatography column developed in this work could be used in the feature for another solute molecule-humic acid binding studies or for a separative mode.

show abstract

Section: Binding Constant Values Determined By Frontal Analysissupporting

confidence: 87%

New liquid chromatography columns for highlighting the interaction of ligand candidates with humic acid

Guillaume

André²

2023

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Previous studies of the interactions of SWs with membranes are limited. In one study (39), artificial membranes immobilized onto silica were used to probe thermodynamic interactions of rodenticides binding to phosphatidyl choline. Similar to our findings, those studies showed that the SWs BDF, DiF, and bromadiolone had the highest binding constant for phosphatidyl choline and that interactions with both the tail and head regions contributed to binding.…”

Section: Discussionmentioning

confidence: 99%

Membrane Cholesterol Modulates Superwarfarin Toxicity

Marangoni

Martynowycz

Kuzmenko

et al. 2016

Biophysical Journal

View full text Add to dashboard Cite

Superwarfarins are modified analogs of warfarin with additional lipophilic aromatic rings, up to 100-fold greater potency, and longer biological half-lives. We hypothesized that increased hydrophobicity allowed interactions with amphiphilic membranes and modulation of biological responses. We find that superwarfarins brodifacoum and difenacoum increase lactate production and cell death in neuroblastoma cells. In contrast, neither causes changes in glioma cells that have higher cholesterol content. After choleterol depletion, lactate production was increased and cell viability was reduced. Drug-membrane interactions were examined by surface X-ray scattering using Langmuir monolayers of dipalmitoylphosphatidylcholine and/or cholesterol. Specular X-ray reflectivity data revealed that superwarfarins, but not warfarin, intercalate between dipalmitoylphosphatidylcholine molecules, whereas grazing incidence X-ray diffraction demonstrated changes in lateral crystalline order of the film. Neither agent showed significant interactions with monolayers containing >20% cholesterol. These findings demonstrate an affinity of superwarfarins to biomembranes and suggest that cellular responses to these agents are regulated by cholesterol content.

show abstract

“…As well, the polar substituent on the compound (−F substituent, two −OH substituents) on the fluvastatin enhanced the lipid surface affinity by increasing hydrogen bonds between the solute molecule and the polar head groups of the IAM surface. This was associated with the lowest entropy state classically attributed to the release of the water molecules surrounding the solute molecule when the fluvastatin was transferred into the phospholipid monolayer [54].…”

Section: Thermodynamic Parametersmentioning

confidence: 94%

Statins (HMG-coenzyme A reductase inhibitors)–biomimetic membrane binding mechanism investigated by molecular chromatography

Sarr

André

Guillaume

2008

Journal of Chromatography B

View full text Add to dashboard Cite

Association mechanism between a series of rodenticide and humic acid: A frontal analysis to support the biological data

Cited by 18 publications

References 18 publications

New liquid chromatography columns for highlighting the interaction of ligand candidates with humic acid

New liquid chromatography columns for highlighting the interaction of ligand candidates with humic acid

Membrane Cholesterol Modulates Superwarfarin Toxicity

Statins (HMG-coenzyme A reductase inhibitors)–biomimetic membrane binding mechanism investigated by molecular chromatography

Contact Info

Product

Resources

About