Association mapping for broomrape resistance in sunflower

Calderón-González, Álvaro; Pérez‐Vich, Begoña; Pouilly, Nicolas; Boniface, Marie‐Claude; Louarn, Johann; Velasco, Leonardo; Muños, Stéphane

doi:10.3389/fpls.2022.1056231

Cited by 6 publications

(4 citation statements)

References 70 publications

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“…Other studies have identified the position of additional resistance genes, such as Or5 gene conferring resistance to broomrape race E in the upper region of Chr 3 (Tang et al 2003;Pérez-Vich et al 2004). This region was also found to be a hotspot for resistance to more virulent populations (races F and G) in studies using QTL detection (Imerovski et al 2019) and GWAS (Calderón-González et al 2023). Furthermore, Imerovski et al (2019) and Calderón-González et al (2023) identified a second genomic region on Chr 3 associated with broomrape resistance, and Calderón-González et al (2023) found potential genetic associations with broomrape resistance on Chrs 5, 10, 13, and 16.…”

Section: Introductionmentioning

confidence: 88%

“…Furthermore, Imerovski et al (2019) and Calderón-González et al (2023) identified a second genomic region on Chr 3 associated with broomrape resistance, and Calderón-González et al (2023) found potential genetic associations with broomrape resistance on Chrs 5, 10, 13, and 16. The biological function of these QTL and/or minor genes remains to be elucidated, although recent studies pointed the possible role in modulating sunflower O. cumana resistance responses of SWEET (Sugars Will Eventually be Exported Transporters) sugar transporter genes (Calderón-González et al 2023), transcription factors of the basic leucine zipper family (Li et al 2023) or genes involved in the biosynthesis of monophenols (4CL2 4-coumarate-CoA ligase (2) or basal defence regulation and salicylic acid signalling activation (EDS1, enhanced disease susceptibility 1) (Huang et al 2022).…”

Section: Introductionmentioning

confidence: 97%

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Development and characterization of a new sunflower source of resistance to race G of Orobanche cumana Wallr. derived from Helianthus anomalus

Fernández-Melero,

del Moral,

Todesco

et al. 2024

Theor Appl Genet

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Key message A new OrAnom1 gene introgressed in cultivated sunflower from wild Helianthus anomalusconfers late post-attachment resistance toOrobanche cumana race G and maps to a target interval in Chromosome 4 where two receptor-like kinases (RLKs) have been identified in theH. anomalusgenome as putative candidates. Abstract Sunflower broomrape is a parasitic weed that infects sunflower (Helianthus annuus L.) roots causing severe yield losses. Breeding for resistance is the most effective and sustainable control method. In this study, we report the identification, introgression, and genetic and physiological characterization of a new sunflower source of resistance to race G of broomrape developed from the wild annual sunflower H. anomalus (accession PI 468642). Crosses between PI 468642 and the susceptible line P21 were carried out, and the genetic study was conducted in BC1F1, BC1F2, and its derived BC1F3 populations. A BC1F5 germplasm named ANOM1 was developed through selection for race G resistance and resemblance to cultivated sunflower. The resistant trait showed monogenic and dominant inheritance. The gene, named OrAnom1, was mapped to Chromosome 4 within a 1.2 cM interval and co-segregated with 7 SNP markers. This interval corresponds to a 1.32 Mb region in the sunflower reference genome, housing a cluster of receptor-like kinase and receptor-like protein (RLK-RLP) genes. Notably, the analysis of the H. anomalus genome revealed the absence of RLPs in the OrAnom1 target region but featured two RLKs as possible OrAnom1 candidates. Rhizotron and histological studies showed that OrAnom1 determines a late post-attachment resistance mechanism. Broomrape can establish a vascular connection with the host, but parasite growth is stopped before tubercle development, showing phenolic compounds accumulation and tubercle necrosis. ANOM1 will contribute to broadening the genetic basis of broomrape resistance in the cultivated sunflower pool and to a better understanding of the molecular basis of the sunflower-broomrape interaction.

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Section: Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 97%

Development and characterization of a new sunflower source of resistance to race G of Orobanche cumana Wallr. derived from Helianthus anomalus

Fernández-Melero,

del Moral,

Todesco

et al. 2024

Theor Appl Genet

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“…The most efficient and economical method for controlling broomrape resistance is crop breeding [ 8 , 9 ]. At present, no broad-spectrum resistant varieties against broomrape have been found, and existing resistant hosts mainly show resistance to specific races; for example, the sunflower P-96 shows dominant resistance to broomrape race E and recessive resistance to the very new race F [ 10 ], and the sunflower HaOr7 mainly controls the Spanish broomrape race F populations [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Identification and genetic diversity analysis of broomrape in Xinjiang, China

Zhang,

Du,

Wang

et al. 2024

Mol Biol Rep

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Background As a holoparasitic weed, broomrape has seriously threatened the production of economically important crops, such as melon, watermelon, processed tomato, and sunflower, in Xinjiang in recent years. However, the distribution and genetic diversity of broomrape populations in Xinjiang are not clear at present, which hinders their prevention and control. The purpose of this study was to identify the main species and the genetic differentiation structure of the broomrape population in Xinjiang. Methods and results In the present study, 93 samples from different geographic regions of Xinjiang were collected to identify the species based on ITS and plastid rps2 regions, and the samples were also used to analyze the genetic diversity based on ISSR markers. The results showed that broomrape is not monophyletic in Xinjiang and consists of two major clades (Orobanche cf. aegyptiaca and O. cernua) and three subclades (O. cf. aegyptiaca var. tch, O. cf. aegyptiaca var. klz, and O. cernua.var. alt) based on phylogenetic analysis. Furthermore, the results of the genetic diversity analysis indicated that the average polymorphic information content and marker index were high values of 0.58 and 7.38, respectively, showing the efficiency of the ISSR markers in detecting polymorphism among the broomrape population studied. Additionally, the 11 selected primers produced 154 repeatable polymorphic bands, of which 150 were polymorphic. The genetic diversity of the samples was 37.19% within populations and 62.81% among the populations, indicating that the main genetic differentiation occurred among the populations. There was less gene exchange between populations, with a gene flow index (Nm) of 0.2961 (< 1). The UPGMA dendrogram indicated that most populations with similar geographical conditions and hosts were clustered first, and then all samples were separated into two major groups and seven subclusters. Conclusion The broomrapes are mainly O. cf. aegyptiaca and O. cernua in Xinjiang, which were separated into two major groups and seven subclusters based on ISSR markers. Our results provide a theoretical basis for breeding broomrape-resistant varieties.

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“…The most e cient and economical method of controlling broomrape resistance is crop breeding [8,9] . It is important to clarify the species and genetic diversity of broomrape populations to cultivate varieties that are resistant to broomrape.…”

Section: Introductionmentioning

confidence: 99%

Identification and genetic diversity analysis of broomrape in Xinjiang, China

Zhang,

Du,

Wang

et al. 2023

Preprint

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Background: As a holoparasitic weed, broomrape has seriously threatened the production of economically important crops, such as melon, watermelon, processed tomato, and sunflower, in the Xinjiang Province in recent years. However, the distribution and genetic diversity of broomrape populations in Xinjiang are not clear at present, which hinders their prevention and control process. The purpose of this study is to identify the main species and the genetic differentiation structure of the broomrape population in Xinjiang province. Methods and Results In the present study, 93 samples from different geographic regions of Xinjiang were collected to identify the species based on ITS and plastid rps2 regions, and the samples were also used to analyze the genetic diversity based on ISSR markers. The results showed that broomrape is not monophyletic in Xinjiang and consists of two major clades (Orobanche cf. aegyptiaca and O. cernua) and three subclades (O. cf. aegyptiaca var. tch, O. cf. aegyptiaca var. klz, O. cernua.var. alt) by phylogenetic analysis based on ITS and rps2. Furthermore, the results of the genetic diversity analysis indicated that the 11 selected primers produced 154 repeatable polymorphic bands, of which 150 were polymorphic. The genetic diversity of the samples was 37.19% within populations and 62.81% among the populations, indicating that the main genetic differentiation occurred among the populations. There was less gene exchange between populations, with a gene flow index (Nm) of 0.2961 (<1). The UPGMA dendrogram indicated that most populations with similar geographical conditions and hosts were clustered first, and then all samples were separated into two major groups and seven subclusters. Conclusion The broomrapes are mainly O. cf. aegyptiacaand O. cernua in Xinjiang province, which were separated into two major groups and seven subclusters furtherly based on ISSR markers. This is the first study to clarify genetic diversity and population distribution of broomrape in Xinjiang. Our results provide a theoretical basis for the prevention, control, and breeding of broomrape-resistant varieties.

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Association mapping for broomrape resistance in sunflower

Cited by 6 publications

References 70 publications

Development and characterization of a new sunflower source of resistance to race G of Orobanche cumana Wallr. derived from Helianthus anomalus

Development and characterization of a new sunflower source of resistance to race G of Orobanche cumana Wallr. derived from Helianthus anomalus

Identification and genetic diversity analysis of broomrape in Xinjiang, China

Identification and genetic diversity analysis of broomrape in Xinjiang, China

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