Association between XRCC1 exon 10 (Arg399Gln) gene polymorphism and micronucleus as a predictor of DNA damage among radiation workers

Abstract

Surniyantoro HNE, Lusiyanti Y, Rahardjo T, Nurhayati S, Tetriana D. 2018. Association between XRCC1 exon 10(Arg399Gln) gene polymorphism and micronucleus as a predictor of DNA damage among radiation workers. Biodiversitas 19: 1676-1682. This study was aimed to examine the association between XRCC1 exon 10 gene polymorphism and micronucleus frequencies inradiation workers and their relation to the confounding factors. This study involved 37 radiation workers and 37 controls from severalhospitals in Indonesia. Genotyping of X-ray cross-complementing group 1 (XRCC1) exon 10 gene polymorphism and micronucleusassay were performed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and Cytokinesis-Block Micronucleus assay (CBMN assay), respectively. The results indicated that MN frequencies were not significantly higher in theexposed workers than in controls (20.46±6.42 versus 16.89 ±9.72; P=0.07). The micronucleus frequencies of radiation workers withmutant genotype showed not significantly higher than controls in the same genotypes (22±6.64 versus 11.75 ± 8.13; P=0.11). Theconfounding factors, like age, years of employment and equivalent doses were significantly associated with micronucleus frequencies(P<0.05). The equivalent dose has a significantly positive correlation with micronucleus frequencies among radiation workers,increasing the MN frequencies by 16.3 per 1 mSv of equivalent dose (P=0.001). The genetic polymorphism of XRCC1 gene exon 10demonstrated no association with the extent of DNA damage in the hospital radiation workers. The MN frequencies were stronglyassociated with age, equivalent dose and years of employment.

The Evaluation of Frequencies of Cytogenetic Biomarkers in Lymphocyte of Residents from High Natural Radiation Area in West Sulawesi, Indonesia

“…The results showed that frequencies of MN are not related to age or gender. Although in our previous study by Surniyantoro et al [34], the confounding factors, like age, years of employment, and equivalent doses were significantly associated with MN frequencies and every extra one year of age, 1.024 (95% CI, 1.018 to 1.030) times more MN were found in radiation workers who have received a radiation exposure of 0.022 to 0.731 mSv. The gender factor also significantly affects MN frequencies in the population who have never received radiation exposure (control, P<0.001).…”

“…The gender factor also significantly affects MN frequencies in the population who have never received radiation exposure (control, P<0.001). Females had an increased risk of MN frequencies of 1.455 (95% CI, 1.213 to 1.745) higher than males [34]. This is under our previous study by Syaifudin et al [15], which states that the frequencies of MN are positively correlated with age, where it corresponds to the decreased efficiency of DNA repair, which is typical in older subjects [15].…”

Study of Immune Response and Malondialdehyde Levels in Irradiated Rats Supplemented with Curcuma xanthorriza Roxb Extract

“…The equivalent dose correlated significantly with an increase in micronuclei frequency (MN) of 16.3 per 1 mSv equivalent dose (P=0.001). In addition, the frequency of MN is closely related to age, equivalent dose, and time of exposure (Surniyantoro et al, 2018).…”

IL-6 and IL-10 Levels in Rats Blood Plasma as Immune Responses Post Radioiodine (I-131) Administration

“…Exposure to I-131 can cause cell death due to its ability to penetrate the nucleus and damage the DNA [ 3 ]. A previous study demonstrated that radiation exposure increases DNA damage through micronucleus formation by 16.3 per 1 mSv equivalent dose [ 4 ]. For safety reasons, it is necessary to use I-131 at a lower dose for radiotherapy but with the same success rate as high doses as reported by Fatima et al [ 5 ] using a dose of 30 mCi but has similar ablation success with a dose of 100 mCi in differentiated thyroid cancer (DTC) patients.…”