Association between up-regulated expression proteins and circulating steroidal hormones in leiomyoma

Ura, Blendi; Scrimin, Federica; Monasta, Lorenzo; Radillo, Oriano; Ricci, Giuseppe

doi:10.1016/j.mehy.2015.07.004

Cited by 3 publications

(4 citation statements)

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“…In our previous study, DES and SERPINA1 were validated by immunohistochemistry as upregulated proteins, which is in line with our present data [ 13 ]. Leiomyoma is a hormonal-dependent tumor, and the expression of proteins is strongly related to hormonal levels [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

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A Proteomic Approach for the Identification of Up-Regulated Proteins Involved in the Metabolic Process of the Leiomyoma

Ura

Scrimin

Arrigoni

et al. 2016

IJMS

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Uterine leiomyoma is the most common benign smooth muscle cell tumor of the uterus. Proteomics is a powerful tool for the analysis of complex mixtures of proteins. In our study, we focused on proteins that were upregulated in the leiomyoma compared to the myometrium. Paired samples of eight leiomyomas and adjacent myometrium were obtained and submitted to two-dimensional gel electrophoresis (2-DE) and mass spectrometry for protein identification and to Western blotting for 2-DE data validation. The comparison between the patterns revealed 24 significantly upregulated (p < 0.05) protein spots, 12 of which were found to be associated with the metabolic processes of the leiomyoma and not with the normal myometrium. The overexpression of seven proteins involved in the metabolic processes of the leiomyoma was further validated by Western blotting and 2D Western blotting. Four of these proteins have never been associated with the leiomyoma before. The 2-DE approach coupled with mass spectrometry, which is among the methods of choice for comparative proteomic studies, identified a number of proteins overexpressed in the leiomyoma and involved in several biological processes, including metabolic processes. A better understanding of the mechanism underlying the overexpression of these proteins may be important for therapeutic purposes.

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Section: Discussionmentioning

confidence: 99%

“…All samples were taken during the proliferative phase. Hormonal levels can modify the expression of proteins, as described in a previous study published by our research group [ 29 ]. The median age of patients was 47 with a minimum of 41 years and a maximum of 52 years.…”

Section: Methodsmentioning

confidence: 99%

A Proteomic Approach for the Identification of Up-Regulated Proteins Involved in the Metabolic Process of the Leiomyoma

Ura

Scrimin

Arrigoni

et al. 2016

IJMS

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“…It has been ascertained that leiomyoma growth is strongly dependent on steroids, growth factors, transforming growth factor-β (TGF-β)/Smad, wingless-type (Wnt)/β-catenin, retinoic acid, and vitamin D (2). We also established that protein expression is associated hormone secretion (3).…”

Section: Introductionmentioning

confidence: 89%

Dysregulated chaperones associated with cell proliferation and negative apoptosis regulation in the uterine leiomyoma

et al. 2018

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Uterine leiomyomas are benign smooth muscle cell tumors that originate from the myometrium. In this study we focus on dysregulated chaperones associated with cell proliferation and apoptosis. Paired tissue samples of 15 leiomyomas and adjacent myometria were obtained and analyzed by two-dimensional gel electrophoresis (2-DE). Mass spectrometry was used for protein identification and western blotting for 2-DE data validation. The values of 6 chaperones were found to be significantly different in the leiomyoma when compared with the myometrium. A total of 4 proteins were upregulated in the leiomyoma and 2 proteins were downregulated. Calreticulin and 78 kDa glucose-regulated protein were further validated by western blotting because the first is considered a marker of cell proliferation, while the second protects against apoptotic cell death. In addition, we also validated the two downregulated proteins heat shock protein β-1 and heat shock 70 kDa protein 1A. Our study shows the existence of a dysregulation of chaperone proteins associated with leiomyoma development. Functional studies are needed to ascertain the role of these chaperones in the leiomyoma. This may be crucial for the further development of specific inhibitors against the activity of these proteins in order to block the growth of the leiomyoma.

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“…during growth, a leiomyoma compresses the surrounding structures (the myometrium and connective tissue), causing the progressive formation of a sort of pseudocapsule, rich in collagen fibers (2), characterized by an abnormal extracellular matrix (EcM) and high interstitial fluid pressure (3). Several factors, such as growth factors, retinoic acid, ovarian hormones and vitamin d, are associated with tumor development (4,5).…”

Section: Introductionmentioning

confidence: 99%

Leiomyoma phosphoproteins involved in inhibition of oxidative stress and synthesis of reactive oxygen species

et al. 2019

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Uterine leiomyomas are benign smooth muscle cell tumors originating from the myometrium. The present study focused on leiomyoma and myometrium phosphoproteome enrichment by using immobilized metal affinity chromatography (IMAc). The phosphoproteome was analyzed by two-dimensional gel electrophoresis coupled with mass spectrometry. Western blotting was used for data validation. The results from IMAC identified 26 proteins significantly differentially phosphorylated in leiomyomas compared with normal myometrium. Three upregulated proteins (peroxiredoxin 2, protein disulfide isomerase family A member 3 and peroxiredoxin 4) were further validated by western blotting. Ingenuity pathway analysis revealed that four phosphoproteins were involved in the inhibition of oxidative stress and synthesis of reactive oxygen species. The present results demonstated for the first time an association between oxidative stress and phosphorylation in leiomyoma development.

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Association between up-regulated expression proteins and circulating steroidal hormones in leiomyoma

Cited by 3 publications

References 4 publications

A Proteomic Approach for the Identification of Up-Regulated Proteins Involved in the Metabolic Process of the Leiomyoma

A Proteomic Approach for the Identification of Up-Regulated Proteins Involved in the Metabolic Process of the Leiomyoma

Dysregulated chaperones associated with cell proliferation and negative apoptosis regulation in the uterine leiomyoma

Leiomyoma phosphoproteins involved in inhibition of oxidative stress and synthesis of reactive oxygen species

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