Association between the seminal plasma proteome and sperm functional traits

Intasqui, Paula; Camargo, Mariana; Antoniassi, Mariana Pereira; Cedenho, A.P.; Carvalho, Valdemir Melechco; Cardozo, Karina Helena Morais; Zylbersztejn, Daniel Suslik; Bertolla, Ricardo Pimenta

doi:10.1016/j.fertnstert.2015.11.005

Cited by 56 publications

(72 citation statements)

References 103 publications

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“…Seminal plasma is a very complex fluid composed of secretions from testis and male genital tract. In addition to previous biochemical studies, proteomic approaches allowed the identification of numerous seminal plasma proteins in the chicken and mammalian species (Atikuzzaman et al, ; Borziak, Álvarez‐Fernández, Karr, Pizzari, & Dorus, ; Brito et al, ; Camargo, Intasqui, & Bertolla, ; Druart et al, ; Intasqui et al, ; Labas et al, ; Perez‐Patino et al, ; Samanta, Parida, Dias, & Agarwal, ). Proteomics profiles reflect sperm functional traits (Intasqui et al, ) and highly differ between species.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to previous biochemical studies, proteomic approaches allowed the identification of numerous seminal plasma proteins in the chicken and mammalian species (Atikuzzaman et al, ; Borziak, Álvarez‐Fernández, Karr, Pizzari, & Dorus, ; Brito et al, ; Camargo, Intasqui, & Bertolla, ; Druart et al, ; Intasqui et al, ; Labas et al, ; Perez‐Patino et al, ; Samanta, Parida, Dias, & Agarwal, ). Proteomics profiles reflect sperm functional traits (Intasqui et al, ) and highly differ between species. A significant number of seminal proteases and protease inhibitors are suspected to participate in the regulation of maturation/activation, release, and protection of sperm (Cesari, Monclus Mde, Tejon, Clementi, & Fornes, ; Gurupriya et al, ; Laflamme & Wolfner, ).…”

Section: Introductionmentioning

confidence: 99%

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The seminal acrosin‐inhibitor ClTI1/SPINK2 is a fertility‐associated marker in the chicken

Thélie

Réhault-Godbert

Poirier

et al. 2019

Molecular Reproduction Devel

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The seminal plasma is a very complex fluid, which surrounds sperm in semen. It contains numerous proteins including proteases and protease inhibitors that regulate proteolytic processes associated with protein activation and degradation. We previously identified a seminal protein, chicken liver trypsin inhibitor 1 (ClTI‐1) over expressed in semen of roosters with high fertility, suggesting a role in male fertility. In the present study, we showed that ClTI‐1 gene is actually SPINK2. Using normal healthy adult roosters, we showed that SPINK2 amount in seminal plasma was positively correlated with male fertility in chicken lines with highly contrasted genetic backgrounds (broiler and layer lines). Using affinity chromatography combined to mass spectrometry analysis and kinetic assays, we demonstrated for the first time that two chicken acrosin isoforms (acrosin and acrosin‐like proteins) are the physiological serine protease targets of SPINK2 inhibitor. SPINK2 transcript was overexpressed all along the male tract, and the protein was present in the lumen as expected for secreted proteins. Altogether, these data emphasize the role of seminal SPINK2 Kazal‐type inhibitor as an important actor of fertility in birds through its inhibitory action on acrosin isoforms proteins.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The seminal acrosin‐inhibitor ClTI1/SPINK2 is a fertility‐associated marker in the chicken

Thélie

Réhault-Godbert

Poirier

et al. 2019

Molecular Reproduction Devel

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“…Several studies were conducted and reported about the biochemical composition of seminal plasma in different domestic livestock species [9-11]. However, limited studies have been conducted in horses and donkeys to find out the biochemical components of seminal plasma.…”

Section: Introductionmentioning

confidence: 99%

Biochemical components of seminal plasma and their correlation to the fresh seminal characteristics in Marwari stallions and Poitou jacks

Mal

Ravi

2017

Vet World

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Aim:To investigate various biochemical components of seminal plasma in Marwari stallions and Poitou Jacks and to find out their correlation with that of the seminal characteristics.Materials and Methods:In this study, semen was collected from six Marwari stallions and six Poitou jacks aged from 4 to 6 years and with known fertility status. The semen collection from the stallions were collected during the breeding season, i.e., between the months of April and June. From the collected semen ejaculates, we estimated the values of some biochemical components, viz., total protein content, total lipid content, and enzymes such as glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), alkaline phosphatase (ALP), acid phosphatase (ACP), and lactate dehydrogenase (LDH) as well as concentrations of glucose, cholesterol, total calcium (Ca), and phosphorus (P) and correlations among different seminal parameters were statistically examined using the Pearson correlation coefficient.Results:In this study, we found positive correlations between semen volume as well as sperm concentration and GOT, GPT, ALP and ACP for both the group stallions. Significant correlation between motility and glucose, GOT and GPT could be an indication for their role metabolism and protection against free radicals to the spermatozoa.Conclusion:Based on the results, it is concluded that there is a positive correlation between some biochemical values such as glucose, Ca, ALP, and LDH and seminal parameters which play a key role in capacitation and onward movement of the spermatozoa.

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“…Mitochondrial activity was performed by staining with 3,3′‐diaminobenzidine (DAB; Antoniassi et al, 2016; Intasqui et al, 2015). This technique is based on the dye oxidation by the cytochrome C complex and the following deposition in the sperm midpiece/mitochondria (Hrudka, 1987).…”

Section: Methodsmentioning

confidence: 99%

Seminal plasma protein networks and enriched functions in varicocele: Effect of smoking

et al. 2020

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To verify a possible synergistic effect of smoking and varicocele on the seminal plasma proteome and biological functions, a cross‐sectional study was performed in 25 smokers and 24 nonsmokers. Samples were used for conventional semen analysis, functional analysis (DNA fragmentation, acrosome integrity and mitochondrial activity) and proteomics by a shotgun approach. Functional enrichment of biological pathways was performed in differentially expressed proteins. Smokers presented lower ejaculate volume (p = .027), percentage of progressively motile spermatozoa (p = .002), total sperm count (p = .039), morphology (p = .001) and higher percentage of immotile spermatozoa (p = .03), round cell (p = .045) and neutrophil count (p = .009). Smokers also presented lower mitochondrial activity and acrosome integrity and higher DNA fragmentation. We identified and quantified 421 proteins in seminal plasma, of which one was exclusive, 21 were overexpressed and 70 were underexpressed in the seminal plasma of smokers. The proteins neprilysin, beta‐defensin 106A and histone H4A were capable of predicting the smoker group. Enriched functions were related to immune function and sperm machinery in testis/epididymis. Based on our findings, we can conclude that cigarette smoking leads to the establishment of inflammatory protein pathways in the testis/epididymis in the presence of varicocele that seems to act in synergy with the toxic components of the cigarette.

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Association between the seminal plasma proteome and sperm functional traits

Cited by 56 publications

References 103 publications

The seminal acrosin‐inhibitor ClTI1/SPINK2 is a fertility‐associated marker in the chicken

The seminal acrosin‐inhibitor ClTI1/SPINK2 is a fertility‐associated marker in the chicken

Biochemical components of seminal plasma and their correlation to the fresh seminal characteristics in Marwari stallions and Poitou jacks

Seminal plasma protein networks and enriched functions in varicocele: Effect of smoking

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